Journal: International Journal of Molecular Sciences

Article Title: Oral Administration of Microencapsulated B. Longum BAA-999 and Lycopene Modulates IGF-1/IGF-1R/IGFBP3 Protein Expressions in a Colorectal Murine Model

doi: 10.3390/ijms20174275

Figure Lengend Snippet: Population of B. longum in the stomach, small intestine, caecum, colon, and feces of CD-1 mice at week 16 of the treatment. Values are Log CFU/g of content in the luminal content, feces ( a ) or adherent to the tissue ( b ) at different gastrointestinal sections, as indicated. The asterisks (*) indicate significant differences compared to the AOM + DSS control group (Dunnett α = 0.050). Each bar represents the mean ± SEM ( n = 6–10 animals per group).

Article Snippet: The culture used was the collection of Bifidobacterium longum (BF) strain BAA-999, provided by ATCC (American type culture collection, Manassas, VA, USA), wich was freeze-dried; the cellular viability was evaluated in a Neubauer chamber (Marienfeld, Brightline 0.0025 mm 2 , depth 0.100 mm, AUS) with trypan blue as a viability contrast colorant.

Techniques: Control

Journal: Applied and Environmental Microbiology

Article Title: Generation of novel prebiotic oligosaccharide pools from fiber drives biological insight in bacterial glycan metabolism

doi: 10.1128/aem.02077-24

Figure Lengend Snippet: Optical density of Bifidobacterium cultures plotted over time. Strains were cultured in ( A ) SBA and ( B ) SBO as a sole carbon source. All strains were cultured in duplicate in mMRSC + 1% carbohydrate. Species names are abbreviated as follows: B. longum subsp. longum (BLL), B. catenulatum subsp. kashiwanohense (BCK), B. adolescentis (BAD), B. pseudocatenulatum (BPS), and B. bifidum (BBI).

Article Snippet: The A locus identified here contains mostly intracellular enzymes and is homologous to the previously characterized arabinan-degrading enzymes encoded by B. longum subsp. suis ATCC 27533 ( ).

Techniques: Cell Culture

Journal: Nutrients

Article Title: Effects of Fructans from Mexican Agave in Newborns Fed with Infant Formula: A Randomized Controlled Trial

doi: 10.3390/nu7115442

Figure Lengend Snippet: Primers and probes used in this study.

Article Snippet: We verified the specificity and the optimal primer and probe concentration for the best amplification efficiency of each TaqMan assay with the following representative DNA reference strain isolates: Bacteroides fragilis (ATCC 25285D, EN-2), Escherichia coli (ATCC 700928D-5, CFT073), Clostridium difficile (ATCC BAA-1382D-5, 630), Shigella flexneri (ATCC 700930D-5, 2457T), Bifidobacterium adolescentis (ATCC 15703D, E194a Variant a), Bifidobacterium breve (ATCC 15700D-5, S1 Variant a), Bifidobacterium infantis (ATCC 15697D-5, S12), Lactobacillus casei (ATCC 334D-5, ATCC 334TM), Lactobacillus acidophilus (ATCC 4357D-5, Pak), Lactobacillus delbrueckii subsp. bulgaricus (ATCC 11842D-5, Lb14), Veillonella parvula (ATCC 10790D-5, Te3) and Enterococcus faecalis (ATCC 700802D-5, V583).

Techniques: Amplification, Sequencing

Journal: Biosensors & bioelectronics

Article Title: Microarray method to monitor 40 intestinal bacterial species in the study of azo dye reduction

doi: 10.1016/j.bios.2004.04.011

Figure Lengend Snippet: Bacteria and the probe numbers in the microarray

Article Snippet: Anaerobic bacteria were cultured at 35 °C in either prereduced anaerobically sterilized (PRAS) Brain Heart Infusion (BIH) broth supplemented with vitamin K and hemin (Remel, Lenexa, KS, USA), inoculated under an oxygen-free cannula using 85% nitrogen, 10% hydrogen and 5% carbon dioxide, or on PRAS brucella blood agar plates supplemented with vitamin K and hemin (Remel). table ft1 table-wrap mode="anchored" t5 caption a7 Number Bacterial species and strain Probe number 1 B. thetaiotaomicron ATCC 29148 1, 2, 3 2 B. vulgatus ATCC 8482 4, 5, 6 3 B. fragilis ATCC 23745 7, 8, 9 4 B. distasonis ATCC 8503 10, 11, 12 5 C. clostridioforme ATCC 29084 13, 14, 15 6 C. leptum ATCC 29065 16, 17, 18 7 F. prausnitzii ATCC 27768 19, 20, 21 8 P. productus ATCC 27340 22, 23, 24 9 R. obeum ATCC 29174 25, 26, 27 10 R. bromii ATCC 27255 28, 29, 30 11 R. callidus ATCC 27760 31, 32, 33 12 R. albus ATCC 27210 34, 35, 36 13 B. longum ATCC 15707 37, 38, 39 14 B. adolescentis ATCC 15703 40, 41, 42 15 B. infantis ATCC 15697 43, 44, 45 16 E. biforme ATCC 27806 46, 47, 48 17 E. aerofaciens ATCC 25986 49, 50, 51 18 L. acidophilus ATCC 4356 52, 53, 54 19 E. coli ATCC 25922 55, 56, 57 20 E. faecium ATCC 19434 58, 59, 60 21 B. uniformis ATCC 8492 61, 62, 63 22 B. ovatus ATCC 8483 64, 65, 66 23 B. caccae ATCC 43185 67, 68, 69 24 C. perfringens ATCC 13124 70, 71, 72 25 C. butyricum ATCC 19398 73, 74, 75 26 C. ramosum ATCC 25582 76, 77, 78 27 C. difficile ATCC 9689 79, 80, 81 28 C. indolis ATCC 25771 82, 83, 84 29 F. russii ATCC 25533 85, 86, 87 30 F. nucleatum ATCC 25586 88, 89, 90 31 B. catenulatum ATCC 27539 91, 92, 93 32 B. angulatum ATCC 27535 94, 95, 96 33 E. rectale ATCC 33656 97, 98, 99 34 E. eligens ATCC 27750 100, 101, 102 35 E. limosum ATCC 8486 103, 104, 105 36 E. lentum ATCC 25553 106, 107, 108 37 L. fermentum ATCC 9338 109, 110, 111 38 E. faecalis ATCC 27274 112, 113, 114 39 P. magnus ATCC 14955 115, 116, 117 40 R. gnavus ATCC 291492 118, 119, 120 Open in a separate window Bacteria and the probe numbers in the microarray 2.2.

Techniques: Bacteria

Journal: Biosensors & bioelectronics

Article Title: Microarray method to monitor 40 intestinal bacterial species in the study of azo dye reduction

doi: 10.1016/j.bios.2004.04.011

Figure Lengend Snippet: Microarray test results read from

Article Snippet: Anaerobic bacteria were cultured at 35 °C in either prereduced anaerobically sterilized (PRAS) Brain Heart Infusion (BIH) broth supplemented with vitamin K and hemin (Remel, Lenexa, KS, USA), inoculated under an oxygen-free cannula using 85% nitrogen, 10% hydrogen and 5% carbon dioxide, or on PRAS brucella blood agar plates supplemented with vitamin K and hemin (Remel). table ft1 table-wrap mode="anchored" t5 caption a7 Number Bacterial species and strain Probe number 1 B. thetaiotaomicron ATCC 29148 1, 2, 3 2 B. vulgatus ATCC 8482 4, 5, 6 3 B. fragilis ATCC 23745 7, 8, 9 4 B. distasonis ATCC 8503 10, 11, 12 5 C. clostridioforme ATCC 29084 13, 14, 15 6 C. leptum ATCC 29065 16, 17, 18 7 F. prausnitzii ATCC 27768 19, 20, 21 8 P. productus ATCC 27340 22, 23, 24 9 R. obeum ATCC 29174 25, 26, 27 10 R. bromii ATCC 27255 28, 29, 30 11 R. callidus ATCC 27760 31, 32, 33 12 R. albus ATCC 27210 34, 35, 36 13 B. longum ATCC 15707 37, 38, 39 14 B. adolescentis ATCC 15703 40, 41, 42 15 B. infantis ATCC 15697 43, 44, 45 16 E. biforme ATCC 27806 46, 47, 48 17 E. aerofaciens ATCC 25986 49, 50, 51 18 L. acidophilus ATCC 4356 52, 53, 54 19 E. coli ATCC 25922 55, 56, 57 20 E. faecium ATCC 19434 58, 59, 60 21 B. uniformis ATCC 8492 61, 62, 63 22 B. ovatus ATCC 8483 64, 65, 66 23 B. caccae ATCC 43185 67, 68, 69 24 C. perfringens ATCC 13124 70, 71, 72 25 C. butyricum ATCC 19398 73, 74, 75 26 C. ramosum ATCC 25582 76, 77, 78 27 C. difficile ATCC 9689 79, 80, 81 28 C. indolis ATCC 25771 82, 83, 84 29 F. russii ATCC 25533 85, 86, 87 30 F. nucleatum ATCC 25586 88, 89, 90 31 B. catenulatum ATCC 27539 91, 92, 93 32 B. angulatum ATCC 27535 94, 95, 96 33 E. rectale ATCC 33656 97, 98, 99 34 E. eligens ATCC 27750 100, 101, 102 35 E. limosum ATCC 8486 103, 104, 105 36 E. lentum ATCC 25553 106, 107, 108 37 L. fermentum ATCC 9338 109, 110, 111 38 E. faecalis ATCC 27274 112, 113, 114 39 P. magnus ATCC 14955 115, 116, 117 40 R. gnavus ATCC 291492 118, 119, 120 Open in a separate window Bacteria and the probe numbers in the microarray 2.2.

Techniques: Microarray