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Image Search Results
Journal: Journal of Advanced Research
Article Title: Gut microbial GABAergic signaling improves stress-associated innate immunity to respiratory viral infection
doi: 10.1016/j.jare.2023.06.008
Figure Lengend Snippet: GABA promotes homeostasis and function of AMs during respiratory viral infection. (A-B, D-G) Murine alveolar cell lines, MH-S cells, were pretreated with 0.01% DMSO or GABA (100 μM) for 30 min, followed by infection with IAV (MOI, 0.01) for 8 hrs. Alternatively, specific antagonists for GABAAR, bicuculline (Bic; 40 μM) or GABABR, saclofen (Sac; 15 μM) were applied. (A) Proliferative percentage by Ki-67 staining; (B) Apoptotic rate by Annexin V/PI staining; (C) Spheroid formation assay of self-renewing potential in sorted AMs treated with 0.01% DMSO or GABA (100 μM), Scale bar, 20 μm; (D) qPCR assay of the indicated cytokines; (E) Mitotracker red and Mitotracker green staining of dysfunctional mitochondria; (F) ATP production; (G) mROS level by mitoSOX staining. (H) Schematic illustration of AMs adoptive transferring for I-M. AMs with or without GABA treatment were adoptively transferred into stress-primed mice, followed by IAV infection (500 pfu/mice, i. t.). (I) Percentage of AMs, (J) Concentrations of total protein, and (K) Viral loads in BALF; (L) qRT-PCR assay of the indicated cytokines in the lung. (M) H&E staining of the lung tissues.Scale bar, 200 μm. Shown are representative images. Data representing two or three independent experiments are shown as means ± SD. * P < 0.05, ** P < 0.01, with Student's t test (two groups) or one-way ANOVA (three or more groups) followed by Bonferroni post hoc test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: Bicuculline (HY-N0219)
Techniques: Infection, Staining, Tube Formation Assay, Transferring, Quantitative RT-PCR
Journal: Neuron
Article Title: Input-specific metaplasticity in the visual cortex requires Homer1a mediated mGluR5 signaling
doi: 10.1016/j.neuron.2019.08.017
Figure Lengend Snippet: Highlights
Article Snippet:
Techniques: Virus, Recombinant, Avidin-Biotin Assay, RNA Extraction, Reverse Transcription, Bicinchoninic Acid Protein Assay, Knock-Out, Knock-In, Software, Real-time Polymerase Chain Reaction, Microscopy, Imaging, Patch Clamp, Isolation, Pore Size, Membrane