bicuculline methobromide Search Results


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Tocris bicuculline methobromide bmi tocris bioscience
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MedChemExpress and saclofen
GABA promotes homeostasis and function of AMs during respiratory viral infection. (A-B, D-G) Murine alveolar cell lines, MH-S cells, were pretreated with 0.01% DMSO or GABA (100 μM) for 30 min, followed by infection with IAV (MOI, 0.01) for 8 hrs. Alternatively, specific antagonists for <t>GABAAR,</t> <t>bicuculline</t> (Bic; 40 μM) or GABABR, <t>saclofen</t> (Sac; 15 μM) were applied. (A) Proliferative percentage by Ki-67 staining; (B) Apoptotic rate by Annexin V/PI staining; (C) Spheroid formation assay of self-renewing potential in sorted AMs treated with 0.01% DMSO or GABA (100 μM), Scale bar, 20 μm; (D) qPCR assay of the indicated cytokines; (E) Mitotracker red and Mitotracker green staining of dysfunctional mitochondria; (F) ATP production; (G) mROS level by mitoSOX staining. (H) Schematic illustration of AMs adoptive transferring for I-M. AMs with or without GABA treatment were adoptively transferred into stress-primed mice, followed by IAV infection (500 pfu/mice, i. t.). (I) Percentage of AMs, (J) Concentrations of total protein, and (K) Viral loads in BALF; (L) qRT-PCR assay of the indicated cytokines in the lung. (M) H&E staining of the lung tissues.Scale bar, 200 μm. Shown are representative images. Data representing two or three independent experiments are shown as means ± SD. * P < 0.05, ** P < 0.01, with Student's t test (two groups) or one-way ANOVA (three or more groups) followed by Bonferroni post hoc test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
And Saclofen, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Tocris bicuculline methobromide
GABA promotes homeostasis and function of AMs during respiratory viral infection. (A-B, D-G) Murine alveolar cell lines, MH-S cells, were pretreated with 0.01% DMSO or GABA (100 μM) for 30 min, followed by infection with IAV (MOI, 0.01) for 8 hrs. Alternatively, specific antagonists for <t>GABAAR,</t> <t>bicuculline</t> (Bic; 40 μM) or GABABR, <t>saclofen</t> (Sac; 15 μM) were applied. (A) Proliferative percentage by Ki-67 staining; (B) Apoptotic rate by Annexin V/PI staining; (C) Spheroid formation assay of self-renewing potential in sorted AMs treated with 0.01% DMSO or GABA (100 μM), Scale bar, 20 μm; (D) qPCR assay of the indicated cytokines; (E) Mitotracker red and Mitotracker green staining of dysfunctional mitochondria; (F) ATP production; (G) mROS level by mitoSOX staining. (H) Schematic illustration of AMs adoptive transferring for I-M. AMs with or without GABA treatment were adoptively transferred into stress-primed mice, followed by IAV infection (500 pfu/mice, i. t.). (I) Percentage of AMs, (J) Concentrations of total protein, and (K) Viral loads in BALF; (L) qRT-PCR assay of the indicated cytokines in the lung. (M) H&E staining of the lung tissues.Scale bar, 200 μm. Shown are representative images. Data representing two or three independent experiments are shown as means ± SD. * P < 0.05, ** P < 0.01, with Student's t test (two groups) or one-way ANOVA (three or more groups) followed by Bonferroni post hoc test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Bicuculline Methobromide, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology bicuculline methobromide
GABA promotes homeostasis and function of AMs during respiratory viral infection. (A-B, D-G) Murine alveolar cell lines, MH-S cells, were pretreated with 0.01% DMSO or GABA (100 μM) for 30 min, followed by infection with IAV (MOI, 0.01) for 8 hrs. Alternatively, specific antagonists for <t>GABAAR,</t> <t>bicuculline</t> (Bic; 40 μM) or GABABR, <t>saclofen</t> (Sac; 15 μM) were applied. (A) Proliferative percentage by Ki-67 staining; (B) Apoptotic rate by Annexin V/PI staining; (C) Spheroid formation assay of self-renewing potential in sorted AMs treated with 0.01% DMSO or GABA (100 μM), Scale bar, 20 μm; (D) qPCR assay of the indicated cytokines; (E) Mitotracker red and Mitotracker green staining of dysfunctional mitochondria; (F) ATP production; (G) mROS level by mitoSOX staining. (H) Schematic illustration of AMs adoptive transferring for I-M. AMs with or without GABA treatment were adoptively transferred into stress-primed mice, followed by IAV infection (500 pfu/mice, i. t.). (I) Percentage of AMs, (J) Concentrations of total protein, and (K) Viral loads in BALF; (L) qRT-PCR assay of the indicated cytokines in the lung. (M) H&E staining of the lung tissues.Scale bar, 200 μm. Shown are representative images. Data representing two or three independent experiments are shown as means ± SD. * P < 0.05, ** P < 0.01, with Student's t test (two groups) or one-way ANOVA (three or more groups) followed by Bonferroni post hoc test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Bicuculline Methobromide, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GABA promotes homeostasis and function of AMs during respiratory viral infection. (A-B, D-G) Murine alveolar cell lines, MH-S cells, were pretreated with 0.01% DMSO or GABA (100 μM) for 30 min, followed by infection with IAV (MOI, 0.01) for 8 hrs. Alternatively, specific antagonists for GABAAR, bicuculline (Bic; 40 μM) or GABABR, saclofen (Sac; 15 μM) were applied. (A) Proliferative percentage by Ki-67 staining; (B) Apoptotic rate by Annexin V/PI staining; (C) Spheroid formation assay of self-renewing potential in sorted AMs treated with 0.01% DMSO or GABA (100 μM), Scale bar, 20 μm; (D) qPCR assay of the indicated cytokines; (E) Mitotracker red and Mitotracker green staining of dysfunctional mitochondria; (F) ATP production; (G) mROS level by mitoSOX staining. (H) Schematic illustration of AMs adoptive transferring for I-M. AMs with or without GABA treatment were adoptively transferred into stress-primed mice, followed by IAV infection (500 pfu/mice, i. t.). (I) Percentage of AMs, (J) Concentrations of total protein, and (K) Viral loads in BALF; (L) qRT-PCR assay of the indicated cytokines in the lung. (M) H&E staining of the lung tissues.Scale bar, 200 μm. Shown are representative images. Data representing two or three independent experiments are shown as means ± SD. * P < 0.05, ** P < 0.01, with Student's t test (two groups) or one-way ANOVA (three or more groups) followed by Bonferroni post hoc test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Journal of Advanced Research

Article Title: Gut microbial GABAergic signaling improves stress-associated innate immunity to respiratory viral infection

doi: 10.1016/j.jare.2023.06.008

Figure Lengend Snippet: GABA promotes homeostasis and function of AMs during respiratory viral infection. (A-B, D-G) Murine alveolar cell lines, MH-S cells, were pretreated with 0.01% DMSO or GABA (100 μM) for 30 min, followed by infection with IAV (MOI, 0.01) for 8 hrs. Alternatively, specific antagonists for GABAAR, bicuculline (Bic; 40 μM) or GABABR, saclofen (Sac; 15 μM) were applied. (A) Proliferative percentage by Ki-67 staining; (B) Apoptotic rate by Annexin V/PI staining; (C) Spheroid formation assay of self-renewing potential in sorted AMs treated with 0.01% DMSO or GABA (100 μM), Scale bar, 20 μm; (D) qPCR assay of the indicated cytokines; (E) Mitotracker red and Mitotracker green staining of dysfunctional mitochondria; (F) ATP production; (G) mROS level by mitoSOX staining. (H) Schematic illustration of AMs adoptive transferring for I-M. AMs with or without GABA treatment were adoptively transferred into stress-primed mice, followed by IAV infection (500 pfu/mice, i. t.). (I) Percentage of AMs, (J) Concentrations of total protein, and (K) Viral loads in BALF; (L) qRT-PCR assay of the indicated cytokines in the lung. (M) H&E staining of the lung tissues.Scale bar, 200 μm. Shown are representative images. Data representing two or three independent experiments are shown as means ± SD. * P < 0.05, ** P < 0.01, with Student's t test (two groups) or one-way ANOVA (three or more groups) followed by Bonferroni post hoc test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Bicuculline (HY-N0219) and saclofen (HY-100813) were purchased from MedChemExpress (MCE) (Monmouth Junction, NJ).

Techniques: Infection, Staining, Tube Formation Assay, Transferring, Quantitative RT-PCR

Highlights

Journal: Neuron

Article Title: Input-specific metaplasticity in the visual cortex requires Homer1a mediated mGluR5 signaling

doi: 10.1016/j.neuron.2019.08.017

Figure Lengend Snippet: Highlights

Article Snippet: Bicuculline methiodide , Enzo , Cat# BML-EA149-0050.

Techniques: Virus, Recombinant, Avidin-Biotin Assay, RNA Extraction, Reverse Transcription, Bicinchoninic Acid Protein Assay, Knock-Out, Knock-In, Software, Real-time Polymerase Chain Reaction, Microscopy, Imaging, Patch Clamp, Isolation, Pore Size, Membrane