Journal: Frontiers in Plant Science

Article Title: SnRK2.10 kinase differentially modulates expression of hub WRKY transcription factors genes under salinity and oxidative stress in Arabidopsis thaliana

doi: 10.3389/fpls.2023.1135240

Figure Lengend Snippet: Localization of H 2 O 2 accumulation in response to salinity. Staining of Arabidopsis leaves with an H 2 O 2 -specific BES- H 2 O 2 -Ac probe for intracellular hydrogen peroxide detection was carried out as described in Materials and Methods. Detection of BES- H 2 O 2 -Ac fluorescence is shown in false green and auto-fluorescence of chlorophyll is depicted in false magenta. The panel consists of images of leaves from control conditions (A–D) ; 30 min of 250 mM NaCl applied to roots (E–H) ; 3 days of 150 mM NaCl applied to roots (I–L) ; 30 min of 150 mM NaCl applied directly to leaves (M–P) ; 30 min of 250 mM NaCl applied directly to leaves (Q–T) . Scale bars: 100 μm in the first two columns and 10 μm in the last three columns. Images were made as a single scan in the first two columns, in the remaining columns merged projections from 14-25 individual optical slices are presented. Six to eight leaves from different plants and from independent biological treatments were observed for each treatment. Images from multiple optical sections were collected from each leaf. The panel shows representative images.

Article Snippet: The undetached leaves were stained with 50 µM BES-H 2 O 2 -Ac probe (Wako Chemicals) in 10 µM PIPES buffer, pH 6.8 for 30 min, cut from the rosette, washed 5 times for 1 min in the above buffer and observed immediately.

Techniques: Staining, Fluorescence

Journal: Frontiers in Plant Science

Article Title: SnRK2.10 kinase differentially modulates expression of hub WRKY transcription factors genes under salinity and oxidative stress in Arabidopsis thaliana

doi: 10.3389/fpls.2023.1135240

Figure Lengend Snippet: Localization of H 2 O 2 accumulation in response to salinity. Staining of Arabidopsis leaves with an H 2 O 2 -specific BES- H 2 O 2 -Ac probe for intracellular hydrogen peroxide detection was carried out as described in Materials and Methods. Detection of BES- H 2 O 2 -Ac fluorescence is shown in false green. All figure captions correspond to the description of .

Article Snippet: The undetached leaves were stained with 50 µM BES-H 2 O 2 -Ac probe (Wako Chemicals) in 10 µM PIPES buffer, pH 6.8 for 30 min, cut from the rosette, washed 5 times for 1 min in the above buffer and observed immediately.

Techniques: Staining, Fluorescence