be0075 Search Results


anti mouse gr 1  (Bio X Cell)
96
Bio X Cell anti mouse gr 1
Anti Mouse Gr 1, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/be0075/pmc07811063-258-12-15?v=Bio+X+Cell
Average 96 stars, based on 1 article reviews
anti mouse gr 1 - by Bioz Stars, 2026-07
96/100 stars
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anti mouse ly6g  (Bio X Cell)
96
Bio X Cell anti mouse ly6g
(A) C57BL/6, Gsdmd -/- and Casp11 -/- mice were infected intraperitoneally with 1 x 10 6 CFU of B . abortus . Mice were sacrificed at 72h, 1 and 2 weeks postinfection, and diluted spleen homogenates were added to BB medium agar plates for CFU determination. (B-D) Spleen cells from infected C57BL/6, Gsdmd -/- and Casp11 -/- mice were stained ex-vivo for flow cytometry analysis. Cells were assessed for CD11b + CD11c + (B), CD11b + F4/80 + (C) and CD11b + <t>Ly6G</t> + (D). Data are mean ± SD of five mice/group. (E) Splenic homogenates from mice C57BL/6, Casp11 -/- and Gsdmd -/- infected with B . abortus were submitted to a myeloperoxidase (MPO) activity assay. Data are mean ± SD of five mice/group. (F) C57BL/6, Casp11 -/- and Gsdmd -/- mice were infected with B . abortus , and 3 days post-infection were inoculated i.v. with Ly-6G PE antibody. Representative images show whole organ ex-vivo confocal images from spleens from each group. Percentage of red fluorescent pixels per organ area is also shown. Scale bar = 100 μm. (G) Analysis of CD62L MFI (median of fluorescence intensity) in C57BL/6, Casp11 -/- and Gsdmd -/- Ly6G + cell population, when stimulated with B . abortus or medium alone (NI). (H) Number of Ly6G + cells expressing IL-17 in 1x10 6 splenocytes of C57BL/6, Casp11 -/- and Gsdmd -/- mice infected two weeks with B . abortus (MOI:100). (I) Analysis of Brucella CFU in neutrophils depleted mice. Prior to and during infection, mice were treated with isotype control or with anti-Ly6G antibody. Spleens were excised at day 7 postinfection and bacterial load was measured. * p <0.05, compared to wild-type mice. The graphs are representative of two independent experiments. DCs: dendritic cells; ns: statistically not significant; NI: non-infected.
Anti Mouse Ly6g, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/be0075/pmc06326519-368-10-14?v=Bio+X+Cell
Average 96 stars, based on 1 article reviews
anti mouse ly6g - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier

Image Search Results


(A) C57BL/6, Gsdmd -/- and Casp11 -/- mice were infected intraperitoneally with 1 x 10 6 CFU of B . abortus . Mice were sacrificed at 72h, 1 and 2 weeks postinfection, and diluted spleen homogenates were added to BB medium agar plates for CFU determination. (B-D) Spleen cells from infected C57BL/6, Gsdmd -/- and Casp11 -/- mice were stained ex-vivo for flow cytometry analysis. Cells were assessed for CD11b + CD11c + (B), CD11b + F4/80 + (C) and CD11b + Ly6G + (D). Data are mean ± SD of five mice/group. (E) Splenic homogenates from mice C57BL/6, Casp11 -/- and Gsdmd -/- infected with B . abortus were submitted to a myeloperoxidase (MPO) activity assay. Data are mean ± SD of five mice/group. (F) C57BL/6, Casp11 -/- and Gsdmd -/- mice were infected with B . abortus , and 3 days post-infection were inoculated i.v. with Ly-6G PE antibody. Representative images show whole organ ex-vivo confocal images from spleens from each group. Percentage of red fluorescent pixels per organ area is also shown. Scale bar = 100 μm. (G) Analysis of CD62L MFI (median of fluorescence intensity) in C57BL/6, Casp11 -/- and Gsdmd -/- Ly6G + cell population, when stimulated with B . abortus or medium alone (NI). (H) Number of Ly6G + cells expressing IL-17 in 1x10 6 splenocytes of C57BL/6, Casp11 -/- and Gsdmd -/- mice infected two weeks with B . abortus (MOI:100). (I) Analysis of Brucella CFU in neutrophils depleted mice. Prior to and during infection, mice were treated with isotype control or with anti-Ly6G antibody. Spleens were excised at day 7 postinfection and bacterial load was measured. * p <0.05, compared to wild-type mice. The graphs are representative of two independent experiments. DCs: dendritic cells; ns: statistically not significant; NI: non-infected.

Journal: PLoS Pathogens

Article Title: Guanylate-binding protein 5 licenses caspase-11 for Gasdermin-D mediated host resistance to Brucella abortus infection

doi: 10.1371/journal.ppat.1007519

Figure Lengend Snippet: (A) C57BL/6, Gsdmd -/- and Casp11 -/- mice were infected intraperitoneally with 1 x 10 6 CFU of B . abortus . Mice were sacrificed at 72h, 1 and 2 weeks postinfection, and diluted spleen homogenates were added to BB medium agar plates for CFU determination. (B-D) Spleen cells from infected C57BL/6, Gsdmd -/- and Casp11 -/- mice were stained ex-vivo for flow cytometry analysis. Cells were assessed for CD11b + CD11c + (B), CD11b + F4/80 + (C) and CD11b + Ly6G + (D). Data are mean ± SD of five mice/group. (E) Splenic homogenates from mice C57BL/6, Casp11 -/- and Gsdmd -/- infected with B . abortus were submitted to a myeloperoxidase (MPO) activity assay. Data are mean ± SD of five mice/group. (F) C57BL/6, Casp11 -/- and Gsdmd -/- mice were infected with B . abortus , and 3 days post-infection were inoculated i.v. with Ly-6G PE antibody. Representative images show whole organ ex-vivo confocal images from spleens from each group. Percentage of red fluorescent pixels per organ area is also shown. Scale bar = 100 μm. (G) Analysis of CD62L MFI (median of fluorescence intensity) in C57BL/6, Casp11 -/- and Gsdmd -/- Ly6G + cell population, when stimulated with B . abortus or medium alone (NI). (H) Number of Ly6G + cells expressing IL-17 in 1x10 6 splenocytes of C57BL/6, Casp11 -/- and Gsdmd -/- mice infected two weeks with B . abortus (MOI:100). (I) Analysis of Brucella CFU in neutrophils depleted mice. Prior to and during infection, mice were treated with isotype control or with anti-Ly6G antibody. Spleens were excised at day 7 postinfection and bacterial load was measured. * p <0.05, compared to wild-type mice. The graphs are representative of two independent experiments. DCs: dendritic cells; ns: statistically not significant; NI: non-infected.

Article Snippet: Neutrophils were depleted by intraperitoneal injection of 100 μg of anti-mouse Ly6G (clone 1A8, BioXcell, West Lebanon, NH, USA) 24 hours before infection i.p. with 1 × 10 6 CFU B . abortus virulent strain S2308.

Techniques: Infection, Staining, Ex Vivo, Flow Cytometry, Activity Assay, Fluorescence, Expressing, Control