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Image Search Results
Journal: Cell reports
Article Title: ΔN-Tp63 Mediates Wnt/β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia
doi: 10.1016/j.celrep.2019.08.063
Figure Lengend Snippet: (A–F) Human immortalized BC (BCIs) kept in air-liquid interface (ALI) culture for up to 4 weeks. Human recombinant R-spondin2 (RSPO2) was used to activate Wnt/β-catenin signaling starting at ALI day 7 (D7). n = 3 cultures per time point and treatment. (A) Confocal imaging of specimens stained for Ac.-α-tubulin (MCCs, blue), CC10 (Club cells, green), and Actin (cell membranes, magenta) show reduced MCC and Club cell numbers in RSPO2-treated cultures. (B) RSPO2 does not reduce the number of ΔN-TP63+ (green) cells. Nuclei (DAPI, blue). (C) Quantification from (A) and (B). Mann Whitney test, not significant, ns (p > 0.05); *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. (D) Quantitative real-time PCR. Expression levels are depicted relative to stage controls. RSPO2 reduces expression of MCC ( FOXJ1 , MCIDAS ) and Club cell ( SCGB1A1 ) markers but increases expression of BC markers ( ΔN-TP63 , KRT5 ). Student’s t test, not significant, ns (p > 0.05); *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. (E and F) Optical orthogonal sections of confocal images. RSPO2-treated cultures display increased epithelial thickness and cells staining for BC markers ΔN-TP63 (green, in E) and KRT5 (green, in F). Related to .
Article Snippet: Immortalized human
Techniques: Recombinant, Imaging, Staining, MANN-WHITNEY, Real-time Polymerase Chain Reaction, Expressing