Journal: Biomedicines

Article Title: Microbubble-Enhanced Focused Ultrasound for Infiltrating Gliomas

doi: 10.3390/biomedicines12061230

Figure Lengend Snippet: Clinical FUS systems.

Article Snippet: Assessment of Safety and Feasibility of ExAblate Blood-Brain Barrier (BBB) Disruption , NCT03551249 , Glioma GBM , InSightec ExAblate system.

Techniques:

Journal: Biomedicines

Article Title: Microbubble-Enhanced Focused Ultrasound for Infiltrating Gliomas

doi: 10.3390/biomedicines12061230

Figure Lengend Snippet: Registered clinical trials using FUS for glioma are separated by glioma type. An overview of ongoing clinical trials for treating HGGs.

Article Snippet: Assessment of Safety and Feasibility of ExAblate Blood-Brain Barrier (BBB) Disruption , NCT03551249 , Glioma GBM , InSightec ExAblate system.

Techniques: Clinical Proteomics, Imaging, Permeability, Disruption, Adjuvant

Journal: BMC Complementary and Alternative Medicine

Article Title: Mucosal and blood-brain barrier transport kinetics of the plant N -alkylamide spilanthol using in vitro and in vivo models

doi: 10.1186/s12906-016-1159-0

Figure Lengend Snippet: Brain influx results of spilanthol, BSA and dermorphin (MTR in mice)

Article Snippet: The BBB-positive control dermorphin was purchased at Bachem (Bubendorf, Switzerland).

Techniques:

Journal: PLoS ONE

Article Title: Blood-brain barrier permeability analysis of plant ceramides

doi: 10.1371/journal.pone.0241640

Figure Lengend Snippet: (A) Schematic representation of an in vitro BBB model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic plant-type ceramides (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; * p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; * p <0.05 by t-test).

Article Snippet: To evaluate the in vitro BBB permeability of plant ceramides, a Rat BBB Culture Model Kit (PharmaCo-Cell Co. Ltd., Nagasaki, Japan), which consist of primary cultures of brain capillary endothelial cells, brain pericytes and astrocytes [ , ], was used.

Techniques: In Vitro, Construct, Liquid Chromatography with Mass Spectroscopy

Journal: PLOS ONE

Article Title: Evaluation of arsenic metabolism and tight junction injury after exposure to arsenite and monomethylarsonous acid using a rat in vitro blood–Brain barrier model

doi: 10.1371/journal.pone.0295154

Figure Lengend Snippet: (A) WB analysis of Nrf2 and HO-1 images after 6 h and 24 h exposure to iAs III (1, 10, and 20 μM) or MMA III (0.1, 1, and 2 μM). EP: vascular endothelial cells and pericyte group; AST: astrocytes. The expression levels of Nrf2 (B) and HO-1 (C) were quantified by densitometry, standardized to β-actin, and compared to the control value of 1. Results are presented as the mean ± SD of three independent experiments (n = 3). Comparison of control and arsenic-treated groups was performed using one-way ANOVAs with Tukey’s post hoc tests. The significance levels were: *p < 0.05; **p < 0.01; ***p < 0.001. Moreover, Student’s t-tests were used to compare expression between EP and AST, and the significance levels were # p < 0.05 and ## p < 0.01. Student’s t-tests were also used to compare 6 h and 24 h treatments, with significance levels of † p < 0.05; †† p < 0.01. Finally, the comparison of iAs III (1 μM) or MMA III (1 μM) exposure groups was performed using one-way ANOVAs with Tukey’s post hoc tests, with a significance level of ‡ p < 0.05.

Article Snippet: Arsenic stock solutions were prepared using cell culture-grade sterile water (Nacalai Tesque, Inc., Kyoto, Japan) and brain capillary endothelial cell culture medium (PharmaCo-Cell Company Ltd, Nagasaki, Japan).

Techniques: Expressing, Control, Comparison