bba3 Search Results


intercellular adhesion molecule  (R&D Systems)
93
R&D Systems intercellular adhesion molecule
Figure 6 Zoledronate suppresses Ang II-induced activation of NF-kB, the expression of adhesion molecules and monocytes adhesions to human aortic endothelial cells. (A) Phosphorylation of p65, <t>ICAM-1,</t> and VCAM-1 protein levels were determined by western blot in cultured human aortic endothelial cells (HAEC). (C–E) Zoledronate significantly reduced Ang II-induced phosphorylation of p65. (B) Confluent HAECs plated on 6-well dishes were incu- bated with zoledronate for 48 h, and the human monocyte cell line, THP-1 cells (labelled with 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetox- ymethyl ester, BCECF-AM, Molecular Probes) were added on each monolayer at a density of 5 × 105 cells/mL dissolved in RPMI1640 containing 1% foetal bovine serum and incubated for 60 min at 378C. Non-adherent cells were removed by carefully washing with cold phosphate-buffered saline twice. The numberofadherentcells wascountedby microscope (×100). Theaveragenumberobtained fromfour fieldswasused as an average value.Thefluorescent intensities were determined by excitation and emission at 490 and 535 nm, respectively (n ¼ 3–5 per group).
Intercellular Adhesion Molecule, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bba3/pm24225494-54-67-71?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
intercellular adhesion molecule - by Bioz Stars, 2026-08
93/100 stars
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anti human icam 1 cd54 antibody mab  (R&D Systems)
95
R&D Systems anti human icam 1 cd54 antibody mab
Figure 4 Flow cytometric analyses of tonsil cells. Quadruple-color labeling of MACS-purified CD32 CD192 CD162 CD142 tonsil cells with CD3- PerCP, CD20-PerCP, CD16- PerCP, CD11c- PE, CD4-APC, and <t>CD54-FITC.</t> Gating on the CD41 cells (R1) showed the three populations of CD41
Anti Human Icam 1 Cd54 Antibody Mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bba3/10__1369_slash_jhc__5a6740__2005-59-4-12?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
anti human icam 1 cd54 antibody mab - by Bioz Stars, 2026-08
95/100 stars
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Image Search Results


Figure 6 Zoledronate suppresses Ang II-induced activation of NF-kB, the expression of adhesion molecules and monocytes adhesions to human aortic endothelial cells. (A) Phosphorylation of p65, ICAM-1, and VCAM-1 protein levels were determined by western blot in cultured human aortic endothelial cells (HAEC). (C–E) Zoledronate significantly reduced Ang II-induced phosphorylation of p65. (B) Confluent HAECs plated on 6-well dishes were incu- bated with zoledronate for 48 h, and the human monocyte cell line, THP-1 cells (labelled with 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetox- ymethyl ester, BCECF-AM, Molecular Probes) were added on each monolayer at a density of 5 × 105 cells/mL dissolved in RPMI1640 containing 1% foetal bovine serum and incubated for 60 min at 378C. Non-adherent cells were removed by carefully washing with cold phosphate-buffered saline twice. The numberofadherentcells wascountedby microscope (×100). Theaveragenumberobtained fromfour fieldswasused as an average value.Thefluorescent intensities were determined by excitation and emission at 490 and 535 nm, respectively (n ¼ 3–5 per group).

Journal: Cardiovascular research

Article Title: Zoledronate attenuates angiotensin II-induced abdominal aortic aneurysm through inactivation of Rho/ROCK-dependent JNK and NF-κB pathway.

doi: 10.1093/cvr/cvt230

Figure Lengend Snippet: Figure 6 Zoledronate suppresses Ang II-induced activation of NF-kB, the expression of adhesion molecules and monocytes adhesions to human aortic endothelial cells. (A) Phosphorylation of p65, ICAM-1, and VCAM-1 protein levels were determined by western blot in cultured human aortic endothelial cells (HAEC). (C–E) Zoledronate significantly reduced Ang II-induced phosphorylation of p65. (B) Confluent HAECs plated on 6-well dishes were incu- bated with zoledronate for 48 h, and the human monocyte cell line, THP-1 cells (labelled with 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetox- ymethyl ester, BCECF-AM, Molecular Probes) were added on each monolayer at a density of 5 × 105 cells/mL dissolved in RPMI1640 containing 1% foetal bovine serum and incubated for 60 min at 378C. Non-adherent cells were removed by carefully washing with cold phosphate-buffered saline twice. The numberofadherentcells wascountedby microscope (×100). Theaveragenumberobtained fromfour fieldswasused as an average value.Thefluorescent intensities were determined by excitation and emission at 490 and 535 nm, respectively (n ¼ 3–5 per group).

Article Snippet: Membranes were probed with monoclonal antibodies against p-p38 (Cell Signaling, #9211), p38 (Cell Signaling, #9212), p-JNK (Cell Signaling, #9251), JNK (Cell Signaling, #9252), p-ERK 1/2 (Cell Signaling, #9106), p-p65 (Cell Signaling, #3033), p65 (Cell Signaling, #3034), vascular endothelial growth factor (Santa Crus, SC7269), Rho (Cell Signaling, #2117), Ras (Cell Signaling, #3965), Rac (Cell Signaling, #2465), p-myosin phosphatase target subunit-1 (p-MYPT-1, Cell Signaling, #5163), MYPT-1 (Cell Signaling, #2634), intercellular adhesion molecule (ICAM, R&D Systems, BBA-3), and VCAM (R&D Systems, BBA-5).

Techniques: Activation Assay, Expressing, Phospho-proteomics, Western Blot, Cell Culture, Incubation, Saline, Microscopy

Figure 4 Flow cytometric analyses of tonsil cells. Quadruple-color labeling of MACS-purified CD32 CD192 CD162 CD142 tonsil cells with CD3- PerCP, CD20-PerCP, CD16- PerCP, CD11c- PE, CD4-APC, and CD54-FITC. Gating on the CD41 cells (R1) showed the three populations of CD41

Journal: Journal of Histochemistry & Cytochemistry

Article Title: Germinal Center Dendritic Cells Express More ICAM-1 Than Extrafollicular Dendritic Cells and ICAM-1/LFA-1 Interactions are Involved in the Capacity of Dendritic Cells to Induce PBMCs Proliferation

doi: 10.1369/jhc.5a6740.2005

Figure Lengend Snippet: Figure 4 Flow cytometric analyses of tonsil cells. Quadruple-color labeling of MACS-purified CD32 CD192 CD162 CD142 tonsil cells with CD3- PerCP, CD20-PerCP, CD16- PerCP, CD11c- PE, CD4-APC, and CD54-FITC. Gating on the CD41 cells (R1) showed the three populations of CD41

Article Snippet: For blocking experiment, blocking anti-human ICAM-1 (CD54) antibody MAb was purchased from R and D Systems Europe (BBIG-I1, BBA3; Abingdon, UK) and was added on DCs 30 min before culture with PBMCs.

Techniques: Labeling, Purification