basic vector Search Results


mouse  (Vector Laboratories)
96
Vector Laboratories mouse
Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
mouse - by Bioz Stars, 2026-03
96/100 stars
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promoterless pseap2 basic vector  (TaKaRa)
95
TaKaRa promoterless pseap2 basic vector
Promoterless Pseap2 Basic Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/promoterless pseap2 basic vector/product/TaKaRa
Average 95 stars, based on 1 article reviews
promoterless pseap2 basic vector - by Bioz Stars, 2026-03
95/100 stars
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pgl3 basic vector  (Addgene inc)
93
Addgene inc pgl3 basic vector
Pgl3 Basic Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgl3 basic vector/product/Addgene inc
Average 93 stars, based on 1 article reviews
pgl3 basic vector - by Bioz Stars, 2026-03
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placz  (TaKaRa)
94
TaKaRa placz
The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. <t>pLacZ</t> and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Placz, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/placz/product/TaKaRa
Average 94 stars, based on 1 article reviews
placz - by Bioz Stars, 2026-03
94/100 stars
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pgem®-t basic vector  (Promega)
90
Promega pgem®-t basic vector
The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. <t>pLacZ</t> and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Pgem® T Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgem®-t basic vector/product/Promega
Average 90 stars, based on 1 article reviews
pgem®-t basic vector - by Bioz Stars, 2026-03
90/100 stars
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pseap2 control plasmid  (Becton Dickinson)
90
Becton Dickinson pseap2 control plasmid
The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. <t>pLacZ</t> and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Pseap2 Control Plasmid, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pseap2 control plasmid/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
pseap2 control plasmid - by Bioz Stars, 2026-03
90/100 stars
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p gl3 basic vector  (Promega)
90
Promega p gl3 basic vector
The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. <t>pLacZ</t> and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
P Gl3 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p gl3 basic vector/product/Promega
Average 90 stars, based on 1 article reviews
p gl3 basic vector - by Bioz Stars, 2026-03
90/100 stars
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pgl4-based luciferase reporter plasmids containing overexpressed lamc1 promoter  (Genechem)
90
Genechem pgl4-based luciferase reporter plasmids containing overexpressed lamc1 promoter
The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. <t>pLacZ</t> and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Pgl4 Based Luciferase Reporter Plasmids Containing Overexpressed Lamc1 Promoter, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgl4-based luciferase reporter plasmids containing overexpressed lamc1 promoter/product/Genechem
Average 90 stars, based on 1 article reviews
pgl4-based luciferase reporter plasmids containing overexpressed lamc1 promoter - by Bioz Stars, 2026-03
90/100 stars
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pqtev vector  (Bio Basic Canada)
90
Bio Basic Canada pqtev vector
The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. <t>pLacZ</t> and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Pqtev Vector, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pqtev vector/product/Bio Basic Canada
Average 90 stars, based on 1 article reviews
pqtev vector - by Bioz Stars, 2026-03
90/100 stars
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pcbr- control vector  (Promega)
90
Promega pcbr- control vector
The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. <t>pLacZ</t> and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Pcbr Control Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcbr- control vector/product/Promega
Average 90 stars, based on 1 article reviews
pcbr- control vector - by Bioz Stars, 2026-03
90/100 stars
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plg3 baisc vectors  (Promega)
90
Promega plg3 baisc vectors
The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. <t>pLacZ</t> and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Plg3 Baisc Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plg3 baisc vectors/product/Promega
Average 90 stars, based on 1 article reviews
plg3 baisc vectors - by Bioz Stars, 2026-03
90/100 stars
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pucm-t vector  (Bio Basic Canada)
90
Bio Basic Canada pucm-t vector
The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. <t>pLacZ</t> and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Pucm T Vector, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pucm-t vector/product/Bio Basic Canada
Average 90 stars, based on 1 article reviews
pucm-t vector - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. pLacZ and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Biochemical and Biophysical Research Communications

Article Title: An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence

doi: 10.1016/j.bbrc.2019.09.115

Figure Lengend Snippet: The N-protein specifically recognizes viral RNA packaging signals from the coronaviridae family. (A) Schematic representation of the packaging signal (PS) sequence regions of coronaviruses (mouse hepatitis virus, human coronavirus, SARS-CoV) and the influenza A virus, as well as the deletion constructs used to probe regions necessary for N-protein binding. Numbers in parenthesis indicate nucleotide lengths of the sequences designated. (B) Effect of N-protein on mouse hepatitis virus PS-lacZ reporter gene expression, and various regions of the PS-RNA sequence (C–G). β -galactosidase activities achieved upon transformation with individual reporter constructs or control vectors, examined in the presence (red; square) and absence (green; triangle) of N-protein, respectively. pLacZ and pTAC are control plasmids that lack expression of either the packaging signal sequence or N-protein, respectively. Results of the ONPG and CPRG assays measuring the interaction of the SARS-CoV N-protein and each packaging signal RNA are shown on the right, as well as a Western blot demonstrating the expression of LacZ ( β -galactosidase) at 4 h after IPTG induction. Alkaline phosphatase (ALP) was probed as a loading control. All experiments were performed in triplicate, and data are presented as the mean ± SD (standard deviation) of three separate experiments. (H) Assay for LacZ translation on X-gal/IPTG agar plates upon co-transformation of JM109 cells with SARS-CoV N-protein and CoV packaging sequences. Minus (-) and N show JM109 transformants containing CoV packaging signal RNAs in the absence and presence of N-protein, respectively. All experiments were performed three times. . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Each packaging sequence segment was digested with Hin d III and Avr II , and subsequently inserted into pLacZ (pLacZ-Basic; Clontech, Shiga, Japan) to generate pLacZ-SARS-PS318, pLacZ-HCoV-PS100, pLacZ-MHV-PS97 and pLacZ-Flu-N(F)-PS57. pLacZ-SARS-PS230 and pLacZ-SARS-PS151 reporter plasmids were generated by inserting PCR amplified DNA products, using pLacZ-SARS-PS315 as the template.

Techniques: Sequencing, Construct, Protein Binding, Expressing, Transformation Assay, Western Blot, Standard Deviation