basement membrane extract Search Results


matrigel  (R&D Systems)
94
R&D Systems matrigel
Matrigel, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cultrex pathclear reduced growth factor bme  (Bio-Techne corporation)
95
Bio-Techne corporation cultrex pathclear reduced growth factor bme
Cultrex Pathclear Reduced Growth Factor Bme, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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basement membrane extract bme  (Trevigen)
94
Trevigen basement membrane extract bme
Figure 9. Kinetics of glycosaminoglycan binding. Microtiter plates were coated with GAG-rich <t>basement</t> <t>membrane</t> extract <t>(BME)</t> and (A) used to examine MCV and HPV capsid affinity by varying the dose (two fold) of VP1 added to wells. (B) BME coated plates were incubated with increasing concentrations (three fold) of heparinase I/III (‘‘HSase’’) or chondroitinase ABC (‘‘CSase’’) prior to adding a single dose of capsids to each well. (C) Heparin or chondroitin-A/C were serially diluted (five fold) in buffer containing capsids prior to analysis of BME binding. The amount of capsid bound to BME was determined by PicoGreen fluorescence detection of encapsidated DNA. The average of two (A), three (B) or four (C) replicates is shown. The curves were fitted using Prism software and error bars represent the standard deviation. doi:10.1371/journal.ppat.1002161.g009
Basement Membrane Extract Bme, supplied by Trevigen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/basement membrane extract bme/product/Trevigen
Average 94 stars, based on 1 article reviews
basement membrane extract bme - by Bioz Stars, 2026-02
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cultrex reduced growth factor basement membrane matrix  (Trevigen)
97
Trevigen cultrex reduced growth factor basement membrane matrix
Figure 9. Kinetics of glycosaminoglycan binding. Microtiter plates were coated with GAG-rich <t>basement</t> <t>membrane</t> extract <t>(BME)</t> and (A) used to examine MCV and HPV capsid affinity by varying the dose (two fold) of VP1 added to wells. (B) BME coated plates were incubated with increasing concentrations (three fold) of heparinase I/III (‘‘HSase’’) or chondroitinase ABC (‘‘CSase’’) prior to adding a single dose of capsids to each well. (C) Heparin or chondroitin-A/C were serially diluted (five fold) in buffer containing capsids prior to analysis of BME binding. The amount of capsid bound to BME was determined by PicoGreen fluorescence detection of encapsidated DNA. The average of two (A), three (B) or four (C) replicates is shown. The curves were fitted using Prism software and error bars represent the standard deviation. doi:10.1371/journal.ppat.1002161.g009
Cultrex Reduced Growth Factor Basement Membrane Matrix, supplied by Trevigen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matrigel r d systems 344500101 coverslip fisher 12  (R&D Systems)
93
R&D Systems matrigel r d systems 344500101 coverslip fisher 12
Figure 9. Kinetics of glycosaminoglycan binding. Microtiter plates were coated with GAG-rich <t>basement</t> <t>membrane</t> extract <t>(BME)</t> and (A) used to examine MCV and HPV capsid affinity by varying the dose (two fold) of VP1 added to wells. (B) BME coated plates were incubated with increasing concentrations (three fold) of heparinase I/III (‘‘HSase’’) or chondroitinase ABC (‘‘CSase’’) prior to adding a single dose of capsids to each well. (C) Heparin or chondroitin-A/C were serially diluted (five fold) in buffer containing capsids prior to analysis of BME binding. The amount of capsid bound to BME was determined by PicoGreen fluorescence detection of encapsidated DNA. The average of two (A), three (B) or four (C) replicates is shown. The curves were fitted using Prism software and error bars represent the standard deviation. doi:10.1371/journal.ppat.1002161.g009
Matrigel R D Systems 344500101 Coverslip Fisher 12, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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cultrex basement membrane extract  (R&D Systems)
96
R&D Systems cultrex basement membrane extract
Figure 9. Kinetics of glycosaminoglycan binding. Microtiter plates were coated with GAG-rich <t>basement</t> <t>membrane</t> extract <t>(BME)</t> and (A) used to examine MCV and HPV capsid affinity by varying the dose (two fold) of VP1 added to wells. (B) BME coated plates were incubated with increasing concentrations (three fold) of heparinase I/III (‘‘HSase’’) or chondroitinase ABC (‘‘CSase’’) prior to adding a single dose of capsids to each well. (C) Heparin or chondroitin-A/C were serially diluted (five fold) in buffer containing capsids prior to analysis of BME binding. The amount of capsid bound to BME was determined by PicoGreen fluorescence detection of encapsidated DNA. The average of two (A), three (B) or four (C) replicates is shown. The curves were fitted using Prism software and error bars represent the standard deviation. doi:10.1371/journal.ppat.1002161.g009
Cultrex Basement Membrane Extract, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cultrex basement membrane extract/product/R&D Systems
Average 96 stars, based on 1 article reviews
cultrex basement membrane extract - by Bioz Stars, 2026-02
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cultrex reduced growth factor basement membrane extract  (R&D Systems)
96
R&D Systems cultrex reduced growth factor basement membrane extract
Figure 9. Kinetics of glycosaminoglycan binding. Microtiter plates were coated with GAG-rich <t>basement</t> <t>membrane</t> extract <t>(BME)</t> and (A) used to examine MCV and HPV capsid affinity by varying the dose (two fold) of VP1 added to wells. (B) BME coated plates were incubated with increasing concentrations (three fold) of heparinase I/III (‘‘HSase’’) or chondroitinase ABC (‘‘CSase’’) prior to adding a single dose of capsids to each well. (C) Heparin or chondroitin-A/C were serially diluted (five fold) in buffer containing capsids prior to analysis of BME binding. The amount of capsid bound to BME was determined by PicoGreen fluorescence detection of encapsidated DNA. The average of two (A), three (B) or four (C) replicates is shown. The curves were fitted using Prism software and error bars represent the standard deviation. doi:10.1371/journal.ppat.1002161.g009
Cultrex Reduced Growth Factor Basement Membrane Extract, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cultrex reduced growth factor basement membrane extract/product/R&D Systems
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cultrex reduced growth factor basement membrane extract - by Bioz Stars, 2026-02
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cultrex pathclear basement membrane extract bme gels  (R&D Systems)
94
R&D Systems cultrex pathclear basement membrane extract bme gels
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Cultrex Pathclear Basement Membrane Extract Bme Gels, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cultrex pathclear basement membrane extract bme gels/product/R&D Systems
Average 94 stars, based on 1 article reviews
cultrex pathclear basement membrane extract bme gels - by Bioz Stars, 2026-02
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cultrex reduced growth factor basement membrane matrix  (R&D Systems)
95
R&D Systems cultrex reduced growth factor basement membrane matrix
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Cultrex Reduced Growth Factor Basement Membrane Matrix, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cultrex reduced growth factor basement membrane matrix/product/R&D Systems
Average 95 stars, based on 1 article reviews
cultrex reduced growth factor basement membrane matrix - by Bioz Stars, 2026-02
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type2  (R&D Systems)
94
R&D Systems type2
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Type2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/type2/product/R&D Systems
Average 94 stars, based on 1 article reviews
type2 - by Bioz Stars, 2026-02
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cultrex coated plates  (R&D Systems)
94
R&D Systems cultrex coated plates
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Cultrex Coated Plates, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cultrex coated plates/product/R&D Systems
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cultrex coated plates - by Bioz Stars, 2026-02
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basement membrane matrix bme type 3  (Trevigen)
94
Trevigen basement membrane matrix bme type 3
Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on <t>Cultrex</t> <t>PathClear</t> basement membrane extract <t>(BME)</t> gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.
Basement Membrane Matrix Bme Type 3, supplied by Trevigen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/basement membrane matrix bme type 3/product/Trevigen
Average 94 stars, based on 1 article reviews
basement membrane matrix bme type 3 - by Bioz Stars, 2026-02
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Image Search Results


Figure 9. Kinetics of glycosaminoglycan binding. Microtiter plates were coated with GAG-rich basement membrane extract (BME) and (A) used to examine MCV and HPV capsid affinity by varying the dose (two fold) of VP1 added to wells. (B) BME coated plates were incubated with increasing concentrations (three fold) of heparinase I/III (‘‘HSase’’) or chondroitinase ABC (‘‘CSase’’) prior to adding a single dose of capsids to each well. (C) Heparin or chondroitin-A/C were serially diluted (five fold) in buffer containing capsids prior to analysis of BME binding. The amount of capsid bound to BME was determined by PicoGreen fluorescence detection of encapsidated DNA. The average of two (A), three (B) or four (C) replicates is shown. The curves were fitted using Prism software and error bars represent the standard deviation. doi:10.1371/journal.ppat.1002161.g009

Journal: PLoS pathogens

Article Title: Glycosaminoglycans and sialylated glycans sequentially facilitate Merkel cell polyomavirus infectious entry.

doi: 10.1371/journal.ppat.1002161

Figure Lengend Snippet: Figure 9. Kinetics of glycosaminoglycan binding. Microtiter plates were coated with GAG-rich basement membrane extract (BME) and (A) used to examine MCV and HPV capsid affinity by varying the dose (two fold) of VP1 added to wells. (B) BME coated plates were incubated with increasing concentrations (three fold) of heparinase I/III (‘‘HSase’’) or chondroitinase ABC (‘‘CSase’’) prior to adding a single dose of capsids to each well. (C) Heparin or chondroitin-A/C were serially diluted (five fold) in buffer containing capsids prior to analysis of BME binding. The amount of capsid bound to BME was determined by PicoGreen fluorescence detection of encapsidated DNA. The average of two (A), three (B) or four (C) replicates is shown. The curves were fitted using Prism software and error bars represent the standard deviation. doi:10.1371/journal.ppat.1002161.g009

Article Snippet: Basement membrane extract (BME) binding assay Cultrex BME PathClear (Trevigen #3432-005-02), a BME preparation derived from murine Engelbreth-Holm-Swarm tumor, was aliquoted and stored according to manufacturer’s instructions.

Techniques: Binding Assay, Membrane, Incubation, Fluorescence, Software, Standard Deviation

Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on Cultrex PathClear basement membrane extract (BME) gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.

Journal: Journal of Virology

Article Title: Development of a Primary Human Cell Model for the Study of Human Cytomegalovirus Replication and Spread within Salivary Epithelium

doi: 10.1128/JVI.01608-18

Figure Lengend Snippet: Establishment of salispheres and primary epithelial lines from human salivary gland. (A) Freshly isolated salivary gland tissues (top) were digested with collagenase-dispase, and single-cell suspensions were then cultured on Cultrex PathClear basement membrane extract (BME) gels, where multicellular salispheres developed within 72 to 96 h (middle). Salispheres were then dissociated, counted, and replated onto Cultrex PathClear BME gels as in the middle panel or plated onto plastic culture dishes to facilitate HCMV infection experiments (bottom). (B) Salispheres were used for mRNA preparation and amylase 1 and hypoxanthine-guanine phosphotransferase (HPRT) expression. (C) Salispheres were then used for more-detailed analyses of salivary gland-specific gene expression. Taken together, the results indicate that the salispheres grown in vitro exhibit gene expression profiles similar to those of salivary tissue in vivo.

Article Snippet: Following digestion and lysis of red blood cells, the salivary cells were plated on Cultrex PathClear basement membrane extract (BME) gels (R&D Systems) and cultured in BEGM growth medium (containing the BEGM bullet kit and 4% charcoal-stripped fetal bovine serum [FBS]), provided by Lonza.

Techniques: Isolation, Cell Culture, Membrane, Infection, Expressing, Gene Expression, In Vitro, In Vivo