bamhi New England Biolabs Search Results


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  • 99
    New England Biolabs bamh i
    Bamh I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bamhi
    Bamhi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9497 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bamhi hf
    Bamhi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 955 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs ecori
    Ecori, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bamhi
    Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs sali hf
    Restriction map of a FLASH plasmid harboring an extension unit Extension units are released from their plasmid vectors using a quadruple digest. In the example shown, the extension unit contains coding sequence for four TALE repeats (colored rectangles). Digestion of the plasmid with BbsI and <t>BamHI</t> releases the unit from the plasmid with appropriate overhangs for use in the FLASH assembly method. Digestion of the plasmid with the additional restriction enzymes XbaI and <t>SalI</t> prevents the released unit fragment religating back into the vector, thereby eliminating the need to gel purify the fragment.
    Sali Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs klenow fragment
    Restriction map of a FLASH plasmid harboring an extension unit Extension units are released from their plasmid vectors using a quadruple digest. In the example shown, the extension unit contains coding sequence for four TALE repeats (colored rectangles). Digestion of the plasmid with BbsI and <t>BamHI</t> releases the unit from the plasmid with appropriate overhangs for use in the FLASH assembly method. Digestion of the plasmid with the additional restriction enzymes XbaI and <t>SalI</t> prevents the released unit fragment religating back into the vector, thereby eliminating the need to gel purify the fragment.
    Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5769 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs vent polymerase
    Restriction map of a FLASH plasmid harboring an extension unit Extension units are released from their plasmid vectors using a quadruple digest. In the example shown, the extension unit contains coding sequence for four TALE repeats (colored rectangles). Digestion of the plasmid with BbsI and <t>BamHI</t> releases the unit from the plasmid with appropriate overhangs for use in the FLASH assembly method. Digestion of the plasmid with the additional restriction enzymes XbaI and <t>SalI</t> prevents the released unit fragment religating back into the vector, thereby eliminating the need to gel purify the fragment.
    Vent Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2569 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs gibson assembly kit
    Restriction map of a FLASH plasmid harboring an extension unit Extension units are released from their plasmid vectors using a quadruple digest. In the example shown, the extension unit contains coding sequence for four TALE repeats (colored rectangles). Digestion of the plasmid with BbsI and <t>BamHI</t> releases the unit from the plasmid with appropriate overhangs for use in the FLASH assembly method. Digestion of the plasmid with the additional restriction enzymes XbaI and <t>SalI</t> prevents the released unit fragment religating back into the vector, thereby eliminating the need to gel purify the fragment.
    Gibson Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1020 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Restriction map of a FLASH plasmid harboring an extension unit Extension units are released from their plasmid vectors using a quadruple digest. In the example shown, the extension unit contains coding sequence for four TALE repeats (colored rectangles). Digestion of the plasmid with BbsI and BamHI releases the unit from the plasmid with appropriate overhangs for use in the FLASH assembly method. Digestion of the plasmid with the additional restriction enzymes XbaI and SalI prevents the released unit fragment religating back into the vector, thereby eliminating the need to gel purify the fragment.

    Journal: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]

    Article Title: Engineering Customized TALE Nucleases (TALENs) and TALE Transcription Factors by Fast Ligation-based Automatable Solid-phase High-throughput (FLASH) Assembly

    doi: 10.1002/0471142727.mb1216s103

    Figure Lengend Snippet: Restriction map of a FLASH plasmid harboring an extension unit Extension units are released from their plasmid vectors using a quadruple digest. In the example shown, the extension unit contains coding sequence for four TALE repeats (colored rectangles). Digestion of the plasmid with BbsI and BamHI releases the unit from the plasmid with appropriate overhangs for use in the FLASH assembly method. Digestion of the plasmid with the additional restriction enzymes XbaI and SalI prevents the released unit fragment religating back into the vector, thereby eliminating the need to gel purify the fragment.

    Article Snippet: ) 100X Bovine Serum Albumin (BSA) (10 mg/ml) (NEB cat. no. B9001S) Restriction enzymes (NEB): BamHI-HF (cat. no. R3136L) XbaI (cat. no. R0145L) BbsI (cat. no. R0539L) SalI-HF (cat. no. R3138L).

    Techniques: Plasmid Preparation, Sequencing