bacterial expression vector pet15b Millipore Search Results


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  • 92
    Millipore expression vector pet15b
    Construction of plasmids and purification of M2 proteins. (A) The synthetic M2eC or 3M2eC genes without hydrophobic region (amino acids 26–55) from PR8 virus were cloned into <t>pET15b</t> vector (B). The recombinant proteins expressed in E. coli were purified by His-tag affinity chromatography and detected by Western blot using M2e-specific monoclonal Ab, 14C2.
    Expression Vector Pet15b, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1050 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector pet15b/product/Millipore
    Average 92 stars, based on 1050 article reviews
    Price from $9.99 to $1999.99
    expression vector pet15b - by Bioz Stars, 2020-04
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    87
    Millipore pet15b bacterial expression vector
    Construction of plasmids and purification of M2 proteins. (A) The synthetic M2eC or 3M2eC genes without hydrophobic region (amino acids 26–55) from PR8 virus were cloned into <t>pET15b</t> vector (B). The recombinant proteins expressed in E. coli were purified by His-tag affinity chromatography and detected by Western blot using M2e-specific monoclonal Ab, 14C2.
    Pet15b Bacterial Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet15b bacterial expression vector/product/Millipore
    Average 87 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    pet15b bacterial expression vector - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    88
    Millipore protein expression vector pet15b
    Construction of plasmids and purification of M2 proteins. (A) The synthetic M2eC or 3M2eC genes without hydrophobic region (amino acids 26–55) from PR8 virus were cloned into <t>pET15b</t> vector (B). The recombinant proteins expressed in E. coli were purified by His-tag affinity chromatography and detected by Western blot using M2e-specific monoclonal Ab, 14C2.
    Protein Expression Vector Pet15b, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein expression vector pet15b/product/Millipore
    Average 88 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    protein expression vector pet15b - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    88
    Millipore vector pet15b
    Construction of plasmids and purification of M2 proteins. (A) The synthetic M2eC or 3M2eC genes without hydrophobic region (amino acids 26–55) from PR8 virus were cloned into <t>pET15b</t> vector (B). The recombinant proteins expressed in E. coli were purified by His-tag affinity chromatography and detected by Western blot using M2e-specific monoclonal Ab, 14C2.
    Vector Pet15b, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 338 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pet15b/product/Millipore
    Average 88 stars, based on 338 article reviews
    Price from $9.99 to $1999.99
    vector pet15b - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    85
    Millipore pet15b novagen bacterial expression vectors
    Construction of plasmids and purification of M2 proteins. (A) The synthetic M2eC or 3M2eC genes without hydrophobic region (amino acids 26–55) from PR8 virus were cloned into <t>pET15b</t> vector (B). The recombinant proteins expressed in E. coli were purified by His-tag affinity chromatography and detected by Western blot using M2e-specific monoclonal Ab, 14C2.
    Pet15b Novagen Bacterial Expression Vectors, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet15b novagen bacterial expression vectors/product/Millipore
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pet15b novagen bacterial expression vectors - by Bioz Stars, 2020-04
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    99
    Millipore pet 15b
    Construction of plasmids and purification of M2 proteins. (A) The synthetic M2eC or 3M2eC genes without hydrophobic region (amino acids 26–55) from PR8 virus were cloned into <t>pET15b</t> vector (B). The recombinant proteins expressed in E. coli were purified by His-tag affinity chromatography and detected by Western blot using M2e-specific monoclonal Ab, 14C2.
    Pet 15b, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2016 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet 15b/product/Millipore
    Average 99 stars, based on 2016 article reviews
    Price from $9.99 to $1999.99
    pet 15b - by Bioz Stars, 2020-04
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    94
    Millipore bacterial expression vector pet 15b
    Construction of plasmids and purification of M2 proteins. (A) The synthetic M2eC or 3M2eC genes without hydrophobic region (amino acids 26–55) from PR8 virus were cloned into <t>pET15b</t> vector (B). The recombinant proteins expressed in E. coli were purified by His-tag affinity chromatography and detected by Western blot using M2e-specific monoclonal Ab, 14C2.
    Bacterial Expression Vector Pet 15b, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial expression vector pet 15b/product/Millipore
    Average 94 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    bacterial expression vector pet 15b - by Bioz Stars, 2020-04
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    98
    Millipore expression vector pet 15b
    (a) E. coli -expressed ScFv in renaturating buffer applied directly to microplates was indicated by γ chain- and Fab-specific antibodies. (b) Analysis of the inhibition of MAb ZX10 binding to rNS3 by NS3-ScFv-pET or <t>pET-15b</t> vector without insert, both expressed and purified from E. coli and diluted in renaturating buffer. Residual binding of ZX10 was indicated by goat anti-mouse immunoglobulin G. OD, optical density.
    Expression Vector Pet 15b, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 571 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector pet 15b/product/Millipore
    Average 98 stars, based on 571 article reviews
    Price from $9.99 to $1999.99
    expression vector pet 15b - by Bioz Stars, 2020-04
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    Image Search Results


    Construction of plasmids and purification of M2 proteins. (A) The synthetic M2eC or 3M2eC genes without hydrophobic region (amino acids 26–55) from PR8 virus were cloned into pET15b vector (B). The recombinant proteins expressed in E. coli were purified by His-tag affinity chromatography and detected by Western blot using M2e-specific monoclonal Ab, 14C2.

    Journal: PLoS ONE

    Article Title: Sublingual Immunization with M2-Based Vaccine Induces Broad Protective Immunity against Influenza

    doi: 10.1371/journal.pone.0027953

    Figure Lengend Snippet: Construction of plasmids and purification of M2 proteins. (A) The synthetic M2eC or 3M2eC genes without hydrophobic region (amino acids 26–55) from PR8 virus were cloned into pET15b vector (B). The recombinant proteins expressed in E. coli were purified by His-tag affinity chromatography and detected by Western blot using M2e-specific monoclonal Ab, 14C2.

    Article Snippet: For the plasmid expressing 3M2eC, the gene was digested with Xho I and BamH I and inserted into the bacterial expression vector pET15b (Novagen, Madison, WI) to express as a fusion of his-tag at the N terminus, resulting in the plasmid pET15b-3M2eC.

    Techniques: Purification, Clone Assay, Plasmid Preparation, Recombinant, Affinity Chromatography, Western Blot

    (a) E. coli -expressed ScFv in renaturating buffer applied directly to microplates was indicated by γ chain- and Fab-specific antibodies. (b) Analysis of the inhibition of MAb ZX10 binding to rNS3 by NS3-ScFv-pET or pET-15b vector without insert, both expressed and purified from E. coli and diluted in renaturating buffer. Residual binding of ZX10 was indicated by goat anti-mouse immunoglobulin G. OD, optical density.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: Characterization of a Monoclonal Antibody and Its Single-Chain Antibody Fragment Recognizing the Nucleoside Triphosphatase/Helicase Domain of the Hepatitis C Virus Nonstructural 3 Protein

    doi:

    Figure Lengend Snippet: (a) E. coli -expressed ScFv in renaturating buffer applied directly to microplates was indicated by γ chain- and Fab-specific antibodies. (b) Analysis of the inhibition of MAb ZX10 binding to rNS3 by NS3-ScFv-pET or pET-15b vector without insert, both expressed and purified from E. coli and diluted in renaturating buffer. Residual binding of ZX10 was indicated by goat anti-mouse immunoglobulin G. OD, optical density.

    Article Snippet: The digested fragment was inserted into the bacterial expression vector pET-15b (Novagen, Madison, Wis.) to give NS3-ScFv-pET.

    Techniques: Inhibition, Binding Assay, Positron Emission Tomography, Plasmid Preparation, Purification

    Analysis of NS3-ScFv-pET expressed in E. coli by use of a silver-stained SDS minigel (right) and by Western blotting (left). Total E. coli lysate of the pET-15b vector without insert (lanes 1 and 7) and lysate of the E. coli pellet containing NS3-ScFv-pET (lanes 2 and 8) or containing the NS3-ScFv-pET eluted from the Ni-NTA column by Tris buffer with 8 M urea at pH 5.0 (lanes 3 and 9) or pH 4.0 (lanes 5 and 11) or with 0.1 M EDTA (lanes 6 and 12) are shown. Lanes 4 and 10, molecular size markers. The Western blot was developed with an Ni-NTA conjugate and the insoluble substrate BCIP-NBT.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: Characterization of a Monoclonal Antibody and Its Single-Chain Antibody Fragment Recognizing the Nucleoside Triphosphatase/Helicase Domain of the Hepatitis C Virus Nonstructural 3 Protein

    doi:

    Figure Lengend Snippet: Analysis of NS3-ScFv-pET expressed in E. coli by use of a silver-stained SDS minigel (right) and by Western blotting (left). Total E. coli lysate of the pET-15b vector without insert (lanes 1 and 7) and lysate of the E. coli pellet containing NS3-ScFv-pET (lanes 2 and 8) or containing the NS3-ScFv-pET eluted from the Ni-NTA column by Tris buffer with 8 M urea at pH 5.0 (lanes 3 and 9) or pH 4.0 (lanes 5 and 11) or with 0.1 M EDTA (lanes 6 and 12) are shown. Lanes 4 and 10, molecular size markers. The Western blot was developed with an Ni-NTA conjugate and the insoluble substrate BCIP-NBT.

    Article Snippet: The digested fragment was inserted into the bacterial expression vector pET-15b (Novagen, Madison, Wis.) to give NS3-ScFv-pET.

    Techniques: Positron Emission Tomography, Staining, Western Blot, Plasmid Preparation