Journal: bioRxiv

Article Title: Regulation of cyanobacterial type IV pilus-dependent functions by interaction between a c-di-GMP receptor and two transcription factors

doi: 10.64898/2026.03.27.713163

Figure Lengend Snippet: A: Volcano plot showing the differentially expressed genes (DEGs) in the ΔcdgR mutant compared to the wild type determined by RNA-seq. DEGs were defined as genes with [log 2 fold change] >1 and log 10 (FDR)<0.05. B-E: Expression of minor pilin genes in the cdgR::Km mutant strain. The cdgR::Km mutant strain was examined for the expression of minor pilin genes. After 24 h of exposure to white-light illumination (75 µmol photons m -2 s -1 ), total RNA was extracted from cells grown in BG11 medium. Three micrograms of RNA were hybridized with radioactively labeled RNA probes targeting the pilA5 mRNA ( B ) and the 5’-UTR of the pilA9 mRNA ( C ). A double-stranded DNA probe that hybridized with Synechocystis 16S rRNA was used as a loading control. Densitometric quantification determined the relative levels of pilA5 ( D ) and pilA9 mRNA ( E ), which were normalized to 16S rRNA levels. Two biological replicates, each with two technical replicates, were performed for the wild type. Four biological replicates, each with two technical replicates, were used for the cdgR::Km mutant experiment.

Article Snippet: Total RNA was extracted, and ribosomal RNA was removed using the Ribo-clean rRNA Depletion Kit (Bacteria) (RN417, Vazyme, Nanjing, China).

Techniques: Mutagenesis, RNA Sequencing, Expressing, Labeling, Control