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Journal:
Article Title: Mutants of Streptomyces clavuligerus with Disruptions in Different Genes for Clavulanic Acid Biosynthesis Produce Large Amounts of Holomycin: Possible Cross-Regulation of Two Unrelated Secondary Metabolic Pathways
doi: 10.1128/JB.184.23.6559-6565.2002
Figure Lengend Snippet: Strains used in this work
Article Snippet: To purify holomycin, TSB medium was inoculated to 5% (vol/vol) with a seed culture in the same medium, and the culture was incubated for 48 h under the conditions indicated above. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strains Characteristics or use Origin or reference S. clavuligerus ATCC 27064 Wild type ATCC a ccaR :: aph Cephamycin C, clavulanic acid-nonproducer (CCA − ), disruption in ccaR , kanamycin resistant (Km r ) 25 claR :: aph Clavulanic acid nonproducer (CA − ), disruption in claR 26 ORF6:: apr Disruption in ORF6, apramycin resistant (Apr r ) 7 ORF6:: apr ccaR :: aph CCA − , disruption in ccaR and ORF6, Km r Apr r 7 ceaS :: aph CA − , disruption in ceaS , Km r 27 RLF3 CA − , disruption in bls , Km r 2 car :: aph CA − , disruption in car , Km r 26 cyp :: aph CA − , disruption in cyp , Km r 17 ORF12:: aph CA − , disruption in ORF12, Km r 17 ORF15:: aph CA − , disruption in ORF15, Km r 17 ORF15:: aph (pIJ699) CA − , disruption in ORF15, Km r Thio r 16 ORF15:: aph (pIJ699-ORF15) CA − , disruption in ORF15, complemented, Km r Thio r 16 cyp :: aph (pVK99) CA − , disruption in cyp , Km r Thio r 16 cyp :: aph (pVK99- cyp ) Disruption in cyp , complemented, Km r Thio r 16 Micrococcus luteus ATCC 9341 Sensitive strain in
Techniques: