Journal: Cell

Article Title: Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated

doi: 10.1016/j.cell.2017.05.025

Figure Lengend Snippet:

Article Snippet: NucleoBond Xtra BAC , Macherey Nagel , Cat#740436.25.

Techniques: Purification, Transduction, Virus, Recombinant, Transfection, Protease Inhibitor, Modification, Western Blot, Construct, Synthesized, Software

Journal:

Article Title: The immunoglobulin-like modules C?3 and ?2 are the minimal units necessary for human IgE-Fc?RI interaction

doi:

Figure Lengend Snippet: (a) Detection of nitrocellulose-blotted recombinant α1-2 and α2. Supernatants from Sf9 cells infected with nonrecombinant baculovirus (lane 1) and from Sf9 cells infected with α1-2 (lane 2) and α2 (lane 3) recombinant baculoviruses were separated by SDS-PAGE and blotted onto nitrocellulose. Recombinant α1-2 (35 kDa) and α2 (16 kDa) are recognized by an anti-hexahistidine mAb in lanes 2 and 3, respectively. (b) Detection of nitrocellulose-blotted recombinant Cε3 with anti-human IgE antibodies. Nitrocellulose-blotted supernatants from Sf9 insect cells infected with nonrecombinant (lane 1) and Cε3III-expressing (lane 2) baculoviruses were exposed to 125I-labeled anti-human IgE antibodies.

Article Snippet: Sf9 cells ( 24 ) were cotransfected with a mixture of the pVL1392 constructs and pAk Bac baculovirus DNA (Promega Corp., Madison, Wisconsin, USA).

Techniques: Recombinant, Infection, SDS Page, Expressing, Labeling

Journal:

Article Title: The immunoglobulin-like modules C?3 and ?2 are the minimal units necessary for human IgE-Fc?RI interaction

doi:

Figure Lengend Snippet: IgE-binding capacity of nitrocellulose-dotted, baculovirus-expressed α-chain constructs. Sf9 cell supernatants containing recombinant α1-2 (a) or α2 (b) construct were dotted on nitrocellulose. Filter-bound proteins were incubated with serum from an atopic patient containing high IgE levels (lanes 1, 4, and 5), with chimeric monoclonal IgE (2), and with a human IgE–containing myeloma supernatant (3), and were detected with 125I-labeled anti-human IgE RAST antibodies (1-3), a 125I-labeled mouse monoclonal anti-human IgE antibody 12 (4), and, for control purposes, a 125I-labeled human α chain (5).

Article Snippet: Sf9 cells ( 24 ) were cotransfected with a mixture of the pVL1392 constructs and pAk Bac baculovirus DNA (Promega Corp., Madison, Wisconsin, USA).

Techniques: Binding Assay, Construct, Recombinant, Incubation, Labeling, Control