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hampsonii atcc baa2463  (ATCC)
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ATCC hampsonii atcc baa2463
18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay
Hampsonii Atcc Baa2463, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: 18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

Techniques: Multiplex Assay

Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

Techniques: Amplification, Control

Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

Techniques: Intra Assay, Inter Assay