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Boster Bio
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OriGene
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Ophir Spiricon LLC
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Boster Bio
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Cosmo Bio USA
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Image Search Results
Journal: Oncology letters
Article Title: Solamargine triggers hepatoma cell death through apoptosis.
doi: 10.3892/ol.2015.3194
Figure Lengend Snippet: Figure 6. (A) Expression of apoptosis‑associated proteins in SMMC7721 and HepG2 cells treated with SM and (B) the statistical results of this analysis. The expression levels of Bcl‑2, Bax, caspase‑3 and caspase‑9 were detected by western blot analysis using β‑actin as a control. (C) Expression levels of proliferation‑associated proteins, ki67 and pcna, also investigated by western blot analysis and (D) the statistical results of this analysis. The results are representative of at least three independent experiments. SM, solamargine.
Article Snippet:
Techniques: Expressing, Western Blot, Control
Journal: Metabolites
Article Title: 2-Hydroxypropyl-β-cyclodextrin Regulates the Epithelial to Mesenchymal Transition in Breast Cancer Cells by Modulating Cholesterol Homeostasis and Endoplasmic Reticulum Stress
doi: 10.3390/metabo11080562
Figure Lengend Snippet: HP-β-CD caused the deposition of cholesterol in ER and elicited ER stress via decreasing the efflux of cholesterol in MDA-MB-231 cells, LXR-623 reversed this effect. ( A ) MDA-MB-231 cells were treated with various concentration of HP-β-CD (0, 2, 4, 6, 8, 10 mmol/L). Expression levels of E-cadherin, Vimentin, GRP78, and CHOP in MDA-MB-231 cells. ( B ) Expression levels of ABCG1, ABCA1, and LXRα in MDA-MB-231 cells. ( C ) MDA-MB-231 cells were treated with various concentration of LXR-623 (0, 1, 3, 5, 7, 9 μmol/L). Viability of MDA-MB-231 cells. ( D ) Expression levels of ABCG1, ABCA1 in MDA-MB-231 cells. ( E ) Expression levels of E-cadherin, Vimentin, GRP78, and CHOP in MDA-MB-231 cells. ( F ) MDA-MB-231 cells were treated with HP-β-CD then with or without LXR-623. Expression levels of CHOP and GRP78. ( G ) Expression levels of ABCA1 and ABCG1. ( H ) Co-staining of Filipin and immunofluorescence analyses for the content of cholesterol in ER. Magnification, ×400. The red fluorescence showed the ER. Scale bars represent 40 μm. The concentration of HP-β-CD and LXR-623 in ( G , H ) are 5 mmol/L and 3 μmol/L, respectively. Data are presented as the mean ± SD ( n = 3). * p < 0.05 versus the control group, ** p < 0.01 versus the control group, ## p < 0.01 versus the HP-β-CD + LXR-623 group.
Article Snippet: The antibodies were as follow: GRP78 (BIP/GRP78; cat. no. 66574-1-ig; 1:4,500; Monoclonal; ProteinTech Group, Inc., Wuhan, China), CHOP (DDIT3; cat. no. AP13063C; Polyclonal; 1:1000; Abgent, Ltd., Suzhou, China),
Techniques: Concentration Assay, Expressing, Staining, Immunofluorescence, Fluorescence, Control