Journal: Clinical Cancer Research

Article Title: BRAF Inhibition Increases Tumor Infiltration by T cells and Enhances the Antitumor Activity of Adoptive Immunotherapy in Mice

doi: 10.1158/1078-0432.ccr-12-1626

Figure Lengend Snippet: Figure 2. Administration of PLX4720 in vivo increases tumor infiltration of adoptively transferred T cells and enhances antitumor responses. A, B6 nude mice (5 mice/group) bearing A375/H-2Db/gp100 tumors were treated with OFL-expressing pmel-1 T cells, along with gp100 peptide-pulsed dendritic cells, by intravenous injection on day 7 after tumor inoculation. Two days after T-cell transfer, PLX4720 or vehicle alone was administered by oral gavage daily for 3 days. Luciferase imaging showing in vivo trafficking of OFL-expressing pmel-1 T cells on day 5 after T-cell transfer. B, summary of quantitative imaging analysis of transferred T cells at the tumor site. Quantification is expressed as the average of photon flux within ROI (, P < 0.05). C, B6 nude mice (5–7 mice/ group) bearing A375/H-2Db/gp100 tumors were treated with pmel-1 T cells and gp100 peptide-pulsed dendritic cells, by intravenous injection on day 10 after tumor inoculation. Two days after T-cell transfer, mice were fed a diet containing PLX4720 or vehicle for 3 days, and tumor growth was monitored over time. D, mouse survival as monitored over time following treatment. Data shown are expressed as mean SEM and are representative of 2 to 3 independent experiments with similar results.

Article Snippet: B6 nude mice were purchased from the Taconic Farms.

Techniques: In Vivo, Expressing, Injection, Luciferase, Imaging

Journal: Clinical Cancer Research

Article Title: BRAF Inhibition Increases Tumor Infiltration by T cells and Enhances the Antitumor Activity of Adoptive Immunotherapy in Mice

doi: 10.1158/1078-0432.ccr-12-1626

Figure Lengend Snippet: Figure 5. Overexpression of hVEGF abrogates increased infiltration of T cells into PLX4720-treated tumors. A, schematic representation of lentiviral vector expressing the hVEGF and RFP genes, separated by an IRES. A375/H-2Db/gp100 melanoma cells were transduced, and VEGF-expressing cells were sorted on the basis of RFP expression. B, tumor cells with or without overexpression of hVEGF were incubated with PLX4720 for 24 hours, and VEGF concentrations in the supernatants were assessed by ELISA. C, B6 nude mice (5 mice/group) bearing A375/H-2Db/gp100 tumors with or without overexpression of hVEGF were treated as described in Fig. 2A. Pictures show representative results of imaging on day 5 after T-cell transfer. D, graphdepicting fold change in luciferace intensity, generated from quantitative imaging analysis of transferred T cells at the tumor site. Data shown are representative of 2 independent experiments with similar results.

Article Snippet: B6 nude mice were purchased from the Taconic Farms.

Techniques: Over Expression, Plasmid Preparation, Expressing, Incubation, Enzyme-linked Immunosorbent Assay, Imaging, Generated