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Image Search Results
Journal: PLoS Genetics
Article Title: HSPB7 prevents cardiac conduction system defect through maintaining intercalated disc integrity
doi: 10.1371/journal.pgen.1006984
Figure Lengend Snippet: (A) Immunoblot analysis of the cardiac muscle showing HSPB7 constitutive expression from the embryonic stages to adulthood (E14.5 to P28) with multiple forms of HSPB7 at different molecular masses (arrows). (B) Subcellular localization of HSPB7 in the cardiac muscle of adult mice. The heart sections were stained with antibodies against HSPB7 (red) and desmoplakin (desmosome), α-actinin (Z-line), myomesin (M-line), N-cadherin (adhering junction), connexin 43 (gap junction), and cardiac-actin (I-bend). HSPB7 mainly localizes at the intercalated discs and is adjacent to the Z-line with a striated pattern. (C) Colocalization of HSPB7 with N-cadherin during development. Heart sections from the embryonic stages to adulthood (E14.5 to P56) were stained with antibodies against HSPB7 (red) and N-cadherin (green). The nucleus was visualized through Hoechst 33342 staining. Insets show the representative areas with higher magnification. Scale bar: 10 μm.
Article Snippet: The primary antibodies used included mouse monoclonal antibodies anti-α-actinin (clone EA-53, Sigma-Aldrich; 1:200.), anti-Actin, cardiac (clone AC1-20.4.2, Sigma-Aldrich; 1:200), anti-connexin43 (clone CXN-6, Sigma-Aldrich; 1:200), antidesmoplakin (clone DP2.15, Millipore Corporation; 1:200),
Techniques: Western Blot, Expressing, Staining