Journal: PLoS ONE

Article Title: Diverse Hematological Malignancies Including Hodgkin-Like Lymphomas Develop in Chimeric MHC Class II Transgenic Mice

doi: 10.1371/journal.pone.0008539

Figure Lengend Snippet: (A–D). Histology and immunohistochemistry of LTCL (all bars equal 50 µm). (A) LTCL consists of uniform cells with scant cytoplasm showing high mitotic and apoptotic rates and the typical starry sky pattern (hematoxylin & eosin, H&E). (B) CD3+ infiltrates in the liver. (C). Tumor cells are positively stained for Tdt (terminal deoxynucleotidyl transferase) and (D) CD3. (E–F) Flow cytometry analysis of T cell receptor Vβ-repertoire on tumor-bearing spleen samples. (E) Monoclonal single positive (SP) CD4+ LTCL. Dot plots - top: gated on CD3+B220− cells: CD4 versus CD8, middle: gated on SP CD4+ T cells showing the Vβ8.2+ tumor cell population and an irrelevant Vβ-chain (Vβ6); bottom: gated on DP CD4/Vβ8.2 as shown in histogram all other Vβ-families were negative. (F) Monoclonal precursor LTCL gated on CD3+ B220− cells: top: CD4 versus CD8, middle: monoclonal DP CD4+CD8+ tumor cells expressing two Vβ-chains (Vβ7&Vβ10), bottom: gated on DP Vβ7/Vβ10 tumor cells, as shown in histogram all other Vβ-families were not expressed. (G) Lymphocytes were stained as for FACS-analyses; top: Vβ7-PE in red, middle: Vβ10-FITC in green, bottom: merged image showing co-localization of Vβ7-PE and Vβ10-FITC as dual T cell receptors on splenic tumor cells with deconvolution microscopy. All bars equal 5 µm.

Article Snippet: Automated immunohistochemical staining (Ventana Medical Systems) was performed as published using the following primary antibodies: CD45R/B220 (BD), CD3, CD79a, Tdt (Dako), CD30 (Chemicon), CD49b (eBioscience), MPO (NeoMarkers).

Techniques: Immunohistochemistry, Staining, Flow Cytometry, Expressing, Microscopy

Journal: PLoS ONE

Article Title: Diverse Hematological Malignancies Including Hodgkin-Like Lymphomas Develop in Chimeric MHC Class II Transgenic Mice

doi: 10.1371/journal.pone.0008539

Figure Lengend Snippet: (A–K) Histology (H&E) and immunohistochemistry (all bars equal 50 µm). (A) Lymphoblastic B cell lymphoma (LBCL) shows blastic nuclei with prominent nucleoli and numerous mitotic figures. (B) Staining for CD79a+. (C) Diffuse large B cell lymphoma (DLBCL) consists of immunoblastic and centroblastic cells and is (D) CD79a+. (E) The histiocyte-associated variant (DLBCL-HA) shows participation of many histiocytes and granulocytes. (F) B220+ DLBCL-HA. (G–I) The splenic marginal zone lymphoma (SMZL) is usually (H) CD79a+ but (I) B220−. (J) H&E of follicular B cell lymphoma. (K–L) Well-differentiated plasmacytomas display (L) CD79+ plasma cells. (M–O) The cellular phenotype of the B natural killer cell lymphoma (BNKL) is confirmed by (N) positively stained CD79a B cells and (O) DP B220/NK1.1 cells in FACS.

Article Snippet: Automated immunohistochemical staining (Ventana Medical Systems) was performed as published using the following primary antibodies: CD45R/B220 (BD), CD3, CD79a, Tdt (Dako), CD30 (Chemicon), CD49b (eBioscience), MPO (NeoMarkers).

Techniques: Immunohistochemistry, Staining, Variant Assay

Journal: PLoS ONE

Article Title: Diverse Hematological Malignancies Including Hodgkin-Like Lymphomas Develop in Chimeric MHC Class II Transgenic Mice

doi: 10.1371/journal.pone.0008539

Figure Lengend Snippet: (A-P+R-T) Histology and immunohistochemistry of Hodgkin-like lymphoma samples in composite compartments, surrounded by activated B and T cells as well as histiocytes. (A–H). H&E staining of (A–D) lymph node and (E–H) spleen sections containing Hodgkin/Reed-Sternberg (H/RS)-like cells (in white boxes). Magnification of characteristic (D+H) giant mononucleated Hodgkin-like and (B+F) multinucleated RS-like cells. (K+L & O+P) CD30+ Hodgkin-like and (I+J & M+N) RS-like cells in (I–L) lymph node and (M–P) spleen sections. (Q) CDR3-region of IgH-transcripts of microdissected H/RS-like cells from five different mice. (R–T) Immunohistochemistry of either B220 positive or negative (R) Hodgkin-like and (S) RS-like cells surrounded by (T) rosettes of CD3+ T cells. All bars equal 50 µm.

Article Snippet: Automated immunohistochemical staining (Ventana Medical Systems) was performed as published using the following primary antibodies: CD45R/B220 (BD), CD3, CD79a, Tdt (Dako), CD30 (Chemicon), CD49b (eBioscience), MPO (NeoMarkers).

Techniques: Immunohistochemistry, Staining

Journal: Frontiers in Pharmacology

Article Title: In vitro and in vivo characterization of oridonin analogs as anti-inflammatory agents that regulate the NF-κB and NLRP3 inflammasome axis

doi: 10.3389/fphar.2025.1512740

Figure Lengend Snippet: Compound 4c alleviated the symptoms of acute lung injury in mice. (A) , Hematoxylin–eosin (HE) staining of lung tissue sections (magnification, ×100). ALI was established by nasal infusion of LPS. BALB/c mice were intraperitoneally injected with oridonin and 4c. (B) , Immunohistochemistry (IHC) staining of lung tissue sections with LCA (CD45) antibody and hematoxylin (magnification, ×300). (C) , Quantitation of the inflammatory cells (LCA positive staining cells) in the lung tissue sections. (D‒E) , RT‒qPCR assays of lung tissues. (F) , WB analysis of lung tissues. (G‒I) , Quantification of the WB results for NLRP3, p-NF-κB and IL-6. All the results are expressed as the average value ± SD of three independent experiments. # indicates that the difference between the LPS and control groups is significant; and * indicates that the difference between the oridonin derivative and LPS groups is significant. Student’s t-test is used to calculate the significance, ####and ****, p < 0.0001; ### and ***, p < 0.001; ## and **, p < 0.01; # and *, p < 0.05.

Article Snippet: The slides were incubated with LCA (CD45) primary antibody (60287-1-lg, Proteintech, United States) overnight at 4°C.

Techniques: Staining, Injection, Immunohistochemistry, Quantitation Assay, Control