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Image Search Results
Journal: International Journal of Cancer
Article Title: Fast enrichment and detection of circulating tumor cells from large volumes of whole blood of breast cancer patients utilizing a functionalized bioaffinity CTC filtration membrane
doi: 10.1002/ijc.70216
Figure Lengend Snippet: Recovery rates of spiking experiments using (A) MCF‐7, (B) MDA‐MB‐231, and (C) SHP‐77 cells. Of each cell line, either 1, 10, or 100 cells were spiked into 7.5 mL of HD blood. Experiments were performed in triplicate. Twenty‐five Hs 578T (D), MDA‐MB‐231 (E), and MDA‐MB‐468 (F) cells were spiked into 7.5 mL of HD blood to analyze the impact of membrane functionalization with an anti‐EGFR antibody on cell lines with intermediate to high EGFR and low to high EpCAM expression. All error bars represent SD.
Article Snippet: After an initial washing step with 20 mL of 0.1% Tween/PBS, the filter, which had been functionalized with streptavidin, was incubated with 1 μg/mL biotinylated anti‐EpCAM‐antibody (R&D Systems) or 1 μg/mL biotinylated
Techniques: Membrane, Expressing