Journal: Cell reports

Article Title: Chronic stress physically spares but functionally impairs innate-like invariant T cells

doi: 10.1016/j.celrep.2021.108979

Figure Lengend Snippet:

Article Snippet: Mouse: B16-F10-Red-FLuc (B16-FLuc) melanoma cells , PerkinElmer , Cat # BW124734.

Techniques: Control, Recombinant, Western Blot, Enzyme-linked Immunosorbent Assay, Staining, In Situ, Selection, cDNA Synthesis, Software

Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Surface Engineering Tumor Cells with Adjuvant-loaded Particles for Use as Cancer Vaccines

doi: 10.1016/j.jconrel.2016.12.036

Figure Lengend Snippet: (A1) B16.F10 cells and (A2) RM11 cells treated or not treated (control) with biotinylated anti-CD29 antibodies (anti-CD29-biotin) followed by strepavidin-PE. (B–F) Validation of cell-particle hybrid assembly from B16.F10 or RM11 cells subsequent to surface biotinylation using anti-CD29-biotin and mixed with streptavidin-coated particles loaded with rhodamine B and washed to remove unbound particles (hybrid). Control involved the same conditions except cells were not treated with anti-CD29-biotin. Validation was performed using: (B1–2 and E1–2): flow cytometry where (B1 and E1) representive (n = 1) and (B2 and E2) mean (n = 3) results from measuring relative mean rhodamine fluorescence intensity (RMFI(Rh)) of (B1 and B2) B16.F10 and (E1 and E2) RM11 cells; (C1–2 and F1–2): laser scanning confocal microscopy showing (C1 and F1) hybrid and (C2 and F2) control cell-particle mixtures for (C1 and C2) B16.F10 and (F1 and F2) RM11 cells (blue = DAPI stained cell nuclei, red = rhodamine-labeled PLGA particles); (D): scanning electron microscopy showing (D1) B16.F10 hybrid and (D2) control cell-particle mixtures (arrows in C1, D1, and F1 indicate particles bound to cell surface). Scale bar= 20 micron for C1–2 and F1–2, 10 microns for D1–2. When applicable, error bars = SD. ** p < 0.01, *** p < 0.001, n = 3.

Article Snippet: For vaccinations, a B16.F10 GM-CSF clone expressing 220 ng GM-CSF/10 6 cells/day was derived by transducing B16.F10 cells with a lentiviral vector encoding murine GM-CSF (AMSBIO, Cambridge, MA).

Techniques: Flow Cytometry, Fluorescence, Confocal Microscopy, Staining, Labeling, Electron Microscopy

Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Surface Engineering Tumor Cells with Adjuvant-loaded Particles for Use as Cancer Vaccines

doi: 10.1016/j.jconrel.2016.12.036

Figure Lengend Snippet: Laser scanning confocal microscopy imaging of cell-particle hybrid uptake by BMDC. Cell-particle hybrids were incubated with BMDC Yellow arrows indicating colocalization of B16.F10 cells (green) and particles (red) inside BMDC (magenta). Magenta: Alexa flour®700 (CD11c) stained BMDC, green: CFSE labeled B16.F10 melanoma cells, red: rhodamine B-labeled PLGA particles, gray: DAPI stained cell nuclei. Scale bar: 20 micron.

Article Snippet: For vaccinations, a B16.F10 GM-CSF clone expressing 220 ng GM-CSF/10 6 cells/day was derived by transducing B16.F10 cells with a lentiviral vector encoding murine GM-CSF (AMSBIO, Cambridge, MA).

Techniques: Confocal Microscopy, Imaging, Incubation, Staining, Labeling

Journal: Drug Design, Development and Therapy

Article Title: Improving anticancer efficacy of (–)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells

doi: 10.2147/DDDT.S58414

Figure Lengend Snippet: Characterization of EGCG-pNG versus cell viabilities after 24 hours of treatment

Article Snippet: B16F10 murine melanoma cells and African green monkey kidney cells (Vero cells, as control cells) were both obtained from the Bioresource Collection and Research Center (BCRC) of the Food Industrial Research and Development Institute, Hsinchu, Taiwan.

Techniques: Zeta Potential Analyzer

Journal: Drug Design, Development and Therapy

Article Title: Improving anticancer efficacy of (–)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells

doi: 10.2147/DDDT.S58414

Figure Lengend Snippet: The effects of EGCG and pNG on Vero and B16F10 cell proliferation. Notes: ( A ) Cells were treated with or without increasing concentrations of EGCG for 24 hours; ( B ) cells were treated with or without increasing concentrations of pNG for 24 hours. Data shown are means ± standard deviations for three samples. Data containing asterisks are significantly different from the control values at ** P <0.01, *** P <0.001. Abbreviations: EGCG, (–)-epigallocatechin-3-gallate; pNG, physical nanogold.

Article Snippet: B16F10 murine melanoma cells and African green monkey kidney cells (Vero cells, as control cells) were both obtained from the Bioresource Collection and Research Center (BCRC) of the Food Industrial Research and Development Institute, Hsinchu, Taiwan.

Techniques: Control

Journal: Drug Design, Development and Therapy

Article Title: Improving anticancer efficacy of (–)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells

doi: 10.2147/DDDT.S58414

Figure Lengend Snippet: Improving anti-proliferative activity of EGCG and pNG in vitro. Notes: ( A ) Morphologies of B16F10 cells treated with or without 25–50 μM EGCG and/or 1.25–2.5 ppm pNG for 24 hours (magnification ×40). Scale bar, 100 μm; ( B ) All the mixtures of EGCG and pNG underwent ultrasound pretreatment and showed significant 24-hour cytotoxicity compared with pNG or EGCG, respectively, in B16F10 but not Vero cells; ( C ) improved cytotoxicity of the EGCG and pNG mixtures was only achieved with ultrasound pretreatment; this was not observed in the mixture without ultrasound pretreatment. The cell viabilities were determined via WST-8 assay. Data shown are mean ± standard deviation for three samples. Data containing asterisks are significantly different from the control values at ** P <0.01; *** P <0.001. Abbreviations: EGCG, (–)-epigallocatechin-3-gallate; pNG, physical nanogold; E25, EGCG 25 μM; E50, EGCG 50 μM; P1.25, pNG 1.25 ppm; P2.5, pNG 2.5 ppm; E25–P1.25, EGCG-pNG 25 μM:1.25 ppm; E50–P2.5, EGCG-pNG 50 μM:2.5 ppm.

Article Snippet: B16F10 murine melanoma cells and African green monkey kidney cells (Vero cells, as control cells) were both obtained from the Bioresource Collection and Research Center (BCRC) of the Food Industrial Research and Development Institute, Hsinchu, Taiwan.

Techniques: Activity Assay, In Vitro, Standard Deviation, Control

Journal: Drug Design, Development and Therapy

Article Title: Improving anticancer efficacy of (–)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells

doi: 10.2147/DDDT.S58414

Figure Lengend Snippet: Enhanced apoptosis induction of EGCG by pNG in B16F10 cells. Notes: ( A ) The fractions of annexin V-positive B16F10 cells were 3.59%±0.30%, 12.63%±0.61%, 20.93%±0.55%, and 25.7%±0.54%, after treatment with pNG 2.5 ppm, EGCG 50 μM, EGCG-pNG 50 μM:2.5 ppm, and Taxol ® (Sigma-Aldrich; St Louis, MO, USA) 0.5 μM, respectively, at 24 hours; ( B ) the fractions of annexin V-positive and PI-negative B16F10 cells were 1.0% ± 0.17%, 3.2% ± 0.23%, 6.5% ± 0.23%, and 11.7% ± 0.41%, after treatment with pNG 2.5 ppm, EGCG 50 μM, EGCG-pNG 50 μM:2.5 ppm, and Taxol ® 0.5 μM, respectively, at 24 hours; ( C ) cells treated with EGCG-pNG for 24 hours expressed more green fluorescence than those treated with EGCG (magnification ×200). Depolarized mitochondria are indicated by green fluorescence (JC-10 monomer), and polarized mitochondria are indicated by orange fluorescence (aggregated JC-10). Cell nuclei are indicated by blue fluorescence coupled with Hoechst 33342 staining. Scale bar, 10 μm; ( D ) cells treated with EGCG-pNG for 24 hours showed a significant increase of caspase-3, -8, and -9 activity compared with those treated with EGCG; ( E ) initiation of time-dependent apoptotic activation by EGCG-pNG treatment via the caspase pathway in B16F10 melanoma cells. Data shown are mean ± standard deviation for three samples. Data containing asterisks are significantly different from the control values at * P <0.05; ** P <0.01; *** P <0.001. Abbreviations: PI, propidium iodide; EGCG, (–)-epigallocatechin-3-gallate; pNG, physical nanogold; P2.5, pNG 2.5 ppm; E50, EGCG 50 μM; E50-P2.5, EGCG-pNG 50 μM:2.5 ppm; ZVAD, Z-VAD-FMK, N-Benzyloxycarbonyl-Val-Ala-Asp (O-Me) fluoromethyl ketone.

Article Snippet: B16F10 murine melanoma cells and African green monkey kidney cells (Vero cells, as control cells) were both obtained from the Bioresource Collection and Research Center (BCRC) of the Food Industrial Research and Development Institute, Hsinchu, Taiwan.

Techniques: Fluorescence, Staining, Activity Assay, Activation Assay, Standard Deviation, Control