Journal: bioRxiv

Article Title: Efficacy and mechanism of actions of cipargamin as an antibabesial drug candidate

doi: 10.1101/2024.07.15.603500

Figure Lengend Snippet: ( A ) and ( B) Dose-dependent growth curve of CIP on B. bovis and B. gibsoni in vitro . Each value represents the mean ± standard deviation (SD) of three independent experiments carried out in triplicate. ( C) Inhibitory effects of CIP and atovaquone (ATO) plus azithromycin (AZI) on the proliferation of B. microti in BALB/c mice. ( D) Hematocrit (HCT) values in mice treated with CIP or ATO plus AZI compared with vehicle-treated mice. ( E) Inhibitory effects of CIP and atovaquone (ATO) plus azithromycin (AZI) on the proliferation of B. rodhaini in BALB/c mice. ( F) Survival rates of CIP-treated, ATO plus AZI-treated, and vehicle-treated mice. The treatment time is shown by arrows, and significant differences ( P < 0.01) between the drug-treated groups and the vehicle-treated control group are indicated by asterisks. The data from one of six individual experiments are expressed as means ± SD. *, P ˂ 0.05; **, P ˂ 0.01.

Article Snippet: For the in vivo studies, B. microti Peabody mjr strain-(ATCC PRA-99) and B. rodhaini Australia strain-infected RBCs (iRBCs), which were collected and diluted with phosphate-buffered saline (PBS) when the parasitemia levels in the donor mice reached ∼20% and 50%, respectively, and were intraperitoneally injected into BALB/c mice.

Techniques: In Vitro, Standard Deviation, Control

Journal: Cell host & microbe

Article Title: Bifidobacteria or fiber protect against diet-induced microbiota-mediated colonic mucus deterioration

doi: 10.1016/j.chom.2017.11.004

Figure Lengend Snippet: Probiotic and prebiotic supplement prevent distinct aspects of diet-mediated mucus defects. (A) After mucus properties were analyzed in a control subset of mice (C, n = 4) the remaining animals were divided into 4 groups (n = 7 per group) and switched to a Western style diet (WSD) at the age of 9 – 11 weeks. Drinking water was supplemented with vehicle control (WSD), 1% (w/v) oligofructose-enriched inulin (I) Bifidobacterium longum NCC2705 (B) or a combination of both (B+I). (B) Metabolic parameters were analyzed 3 weeks after the dietary switch and mucus measurements performed after 4 weeks on supplemented WSD. (C) Confocal z-stacks (top) calculated from the position of fluorescent 1 μm beads (bottom) were used to determine penetrability of the inner colonic mucus layer (D). The median z-stack is shown for each mouse. Turquoise: colonic tissue; red: bacteria-sized beads. (E) Growth rate and thickness (F) of the inner colonic mucus layer. Data are presented as mean ± SEM. Statistical significance against WSD fed mice (vehicle control) was determined by Kruskal-Wallis test and corrected by Dunn’s multiple comparisons test with (*) = p<0.05 and (**) = p<0.01.

Article Snippet: Sampling time during the day was matched between dietary/genetic groups and all experiments were performed using protocols approved by the University of Gothenburg Animal Studies Committee. table ft1 table-wrap mode="anchored" t5 caption a7 Figure Breeding Facility acclimatisation Littermates Male Female Western diet Mucus measurements WSD , , in-house X X 8 weeks Mucus measurements Ob/Ob S1 in-house X X X -- Microbiota analyses WSD Charles River X X 8 weeks Mucus phenotype time course , Charles River X X 1–28 days Mucus phenotype prevention , , S2 Charles River X X 6 weeks Bifidobacterium/inulin treatment Charles River X X 4 weeks Open in a separate window Source, housing conditions and experimental setup of mice Microbial strains Bifidobacterium (B.) longum NCC2705 was kindly provided by Nestec Ltd., Nestlé Research Center (Lausanne, Switzerland).

Techniques: Control, Western Blot, Bacteria

Journal: Cell host & microbe

Article Title: Bifidobacteria or fiber protect against diet-induced microbiota-mediated colonic mucus deterioration

doi: 10.1016/j.chom.2017.11.004

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Sampling time during the day was matched between dietary/genetic groups and all experiments were performed using protocols approved by the University of Gothenburg Animal Studies Committee. table ft1 table-wrap mode="anchored" t5 caption a7 Figure Breeding Facility acclimatisation Littermates Male Female Western diet Mucus measurements WSD , , in-house X X 8 weeks Mucus measurements Ob/Ob S1 in-house X X X -- Microbiota analyses WSD Charles River X X 8 weeks Mucus phenotype time course , Charles River X X 1–28 days Mucus phenotype prevention , , S2 Charles River X X 6 weeks Bifidobacterium/inulin treatment Charles River X X 4 weeks Open in a separate window Source, housing conditions and experimental setup of mice Microbial strains Bifidobacterium (B.) longum NCC2705 was kindly provided by Nestec Ltd., Nestlé Research Center (Lausanne, Switzerland).

Techniques: Virus, Recombinant, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Plasmid Preparation, SYBR Green Assay, Picogreen Assay, Software, Sequencing, Mass Spectrometry, Spectrophotometry, Real-time Polymerase Chain Reaction, Imaging