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Identification of cardiac fibroblasts and the effect of CHIR99021 and FGF2 on cell viability. ( A ) Immunofluorescence detection of Vimentin and <t>DDR2</t> expression in purified CFs. Scale bar: 100 μm. ( B ) Quantitative analysis of Vimentin- and DDR2- positive cells. ( C ) Effect of CHIR99021 at different concentrations on CF viability, assessed by CCK-8 assay. ( D ) Effect of CHIR99021 and FGF2 on CF viability, evaluated using Calcein AM staining. Scale bar: 100 μm. ( E ) Quantification of Calcein AM fluorescence intensity. n = 3. * p < 0.05, *** p < 0.001.
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Identification of cardiac fibroblasts and the effect of CHIR99021 and FGF2 on cell viability. ( A ) Immunofluorescence detection of Vimentin and <t>DDR2</t> expression in purified CFs. Scale bar: 100 μm. ( B ) Quantitative analysis of Vimentin- and DDR2- positive cells. ( C ) Effect of CHIR99021 at different concentrations on CF viability, assessed by CCK-8 assay. ( D ) Effect of CHIR99021 and FGF2 on CF viability, evaluated using Calcein AM staining. Scale bar: 100 μm. ( E ) Quantification of Calcein AM fluorescence intensity. n = 3. * p < 0.05, *** p < 0.001.
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Identification of cardiac fibroblasts and the effect of CHIR99021 and FGF2 on cell viability. ( A ) Immunofluorescence detection of Vimentin and <t>DDR2</t> expression in purified CFs. Scale bar: 100 μm. ( B ) Quantitative analysis of Vimentin- and DDR2- positive cells. ( C ) Effect of CHIR99021 at different concentrations on CF viability, assessed by CCK-8 assay. ( D ) Effect of CHIR99021 and FGF2 on CF viability, evaluated using Calcein AM staining. Scale bar: 100 μm. ( E ) Quantification of Calcein AM fluorescence intensity. n = 3. * p < 0.05, *** p < 0.001.
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Identification of cardiac fibroblasts and the effect of CHIR99021 and FGF2 on cell viability. ( A ) Immunofluorescence detection of Vimentin and <t>DDR2</t> expression in purified CFs. Scale bar: 100 μm. ( B ) Quantitative analysis of Vimentin- and DDR2- positive cells. ( C ) Effect of CHIR99021 at different concentrations on CF viability, assessed by CCK-8 assay. ( D ) Effect of CHIR99021 and FGF2 on CF viability, evaluated using Calcein AM staining. Scale bar: 100 μm. ( E ) Quantification of Calcein AM fluorescence intensity. n = 3. * p < 0.05, *** p < 0.001.
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ProSci Incorporated anti spike s1 antibody
Identification of cardiac fibroblasts and the effect of CHIR99021 and FGF2 on cell viability. ( A ) Immunofluorescence detection of Vimentin and <t>DDR2</t> expression in purified CFs. Scale bar: 100 μm. ( B ) Quantitative analysis of Vimentin- and DDR2- positive cells. ( C ) Effect of CHIR99021 at different concentrations on CF viability, assessed by CCK-8 assay. ( D ) Effect of CHIR99021 and FGF2 on CF viability, evaluated using Calcein AM staining. Scale bar: 100 μm. ( E ) Quantification of Calcein AM fluorescence intensity. n = 3. * p < 0.05, *** p < 0.001.
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ProSci Incorporated rbd domain
Identification of cardiac fibroblasts and the effect of CHIR99021 and FGF2 on cell viability. ( A ) Immunofluorescence detection of Vimentin and <t>DDR2</t> expression in purified CFs. Scale bar: 100 μm. ( B ) Quantitative analysis of Vimentin- and DDR2- positive cells. ( C ) Effect of CHIR99021 at different concentrations on CF viability, assessed by CCK-8 assay. ( D ) Effect of CHIR99021 and FGF2 on CF viability, evaluated using Calcein AM staining. Scale bar: 100 μm. ( E ) Quantification of Calcein AM fluorescence intensity. n = 3. * p < 0.05, *** p < 0.001.
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Boster Bio tram 34
Identification of cardiac fibroblasts and the effect of CHIR99021 and FGF2 on cell viability. ( A ) Immunofluorescence detection of Vimentin and <t>DDR2</t> expression in purified CFs. Scale bar: 100 μm. ( B ) Quantitative analysis of Vimentin- and DDR2- positive cells. ( C ) Effect of CHIR99021 at different concentrations on CF viability, assessed by CCK-8 assay. ( D ) Effect of CHIR99021 and FGF2 on CF viability, evaluated using Calcein AM staining. Scale bar: 100 μm. ( E ) Quantification of Calcein AM fluorescence intensity. n = 3. * p < 0.05, *** p < 0.001.
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Image Search Results


Identification of cardiac fibroblasts and the effect of CHIR99021 and FGF2 on cell viability. ( A ) Immunofluorescence detection of Vimentin and DDR2 expression in purified CFs. Scale bar: 100 μm. ( B ) Quantitative analysis of Vimentin- and DDR2- positive cells. ( C ) Effect of CHIR99021 at different concentrations on CF viability, assessed by CCK-8 assay. ( D ) Effect of CHIR99021 and FGF2 on CF viability, evaluated using Calcein AM staining. Scale bar: 100 μm. ( E ) Quantification of Calcein AM fluorescence intensity. n = 3. * p < 0.05, *** p < 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Serpine1 Regulates the Enhanced Inhibitory Effect of CHIR99021 Combined with Fibroblast Growth Factor 2 on Myocardial Fibrosis After Myocardial Infarction in Mice

doi: 10.3390/ijms27041627

Figure Lengend Snippet: Identification of cardiac fibroblasts and the effect of CHIR99021 and FGF2 on cell viability. ( A ) Immunofluorescence detection of Vimentin and DDR2 expression in purified CFs. Scale bar: 100 μm. ( B ) Quantitative analysis of Vimentin- and DDR2- positive cells. ( C ) Effect of CHIR99021 at different concentrations on CF viability, assessed by CCK-8 assay. ( D ) Effect of CHIR99021 and FGF2 on CF viability, evaluated using Calcein AM staining. Scale bar: 100 μm. ( E ) Quantification of Calcein AM fluorescence intensity. n = 3. * p < 0.05, *** p < 0.001.

Article Snippet: CF-specific markers Vimentin (ab92547, Abcam, 1:250, Shanghai, China) and DDR2 (A01698-1, Boster, 1:200, Wuhan, China) were detected by immunofluorescence staining.

Techniques: Immunofluorescence, Expressing, Purification, CCK-8 Assay, Staining, Fluorescence