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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Serpine1 Regulates the Enhanced Inhibitory Effect of CHIR99021 Combined with Fibroblast Growth Factor 2 on Myocardial Fibrosis After Myocardial Infarction in Mice
doi: 10.3390/ijms27041627
Figure Lengend Snippet: Identification of cardiac fibroblasts and the effect of CHIR99021 and FGF2 on cell viability. ( A ) Immunofluorescence detection of Vimentin and DDR2 expression in purified CFs. Scale bar: 100 μm. ( B ) Quantitative analysis of Vimentin- and DDR2- positive cells. ( C ) Effect of CHIR99021 at different concentrations on CF viability, assessed by CCK-8 assay. ( D ) Effect of CHIR99021 and FGF2 on CF viability, evaluated using Calcein AM staining. Scale bar: 100 μm. ( E ) Quantification of Calcein AM fluorescence intensity. n = 3. * p < 0.05, *** p < 0.001.
Article Snippet: CF-specific markers Vimentin (ab92547, Abcam, 1:250, Shanghai, China) and
Techniques: Immunofluorescence, Expressing, Purification, CCK-8 Assay, Staining, Fluorescence