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Image Search Results
Journal:
Article Title: Ovarian Cancer Cell Detachment and Multicellular Aggregate Formation Are Regulated by MT1-MMP: A Potential Role in Intra-Peritoneal Metastatic Dissemination
doi: 10.1158/0008-5472.CAN-08-4151
Figure Lengend Snippet: (A) Expression of MT1-MMP RNA in DOV13 MCAs or 2D cultures. Real time RT-PCR was performed to detect RNA expression. Shown is the average of three independent experiments ± standard deviation. (*p<.05). (B) Expression of MT1-MMP protein. Western blot was performed to detect relative MT1-MMP protein levels with β-tubulin as a loading control. Arrow - 55 kDa active MT1-MMP; arrowhead - 43 kDa MT1-MMP autolysis product. (C) Representative MCA formed by OVCA 433 or DOV13 cells. (D) Expression of MT1-MMP augments MCA formation. Cells were cultured for MCA formation using the hanging drop method and measured using a Zeiss Axiovert Imaging Software. Data shown are expressed in μm, and represent the mean diameter measurement of n=10 MCAs. (+) indicates p<.05 relative to vector-transfected cells; (*) indicates p<.05 relative to cells expressing wild type MT1-MMP. Untransfected DOV13 cells, that express similar levels of endogenous MT1-MMP, are shown for comparison.
Article Snippet: Cells were cultured for MCA formation using the hanging drop method and measured using a
Techniques: Expressing, Quantitative RT-PCR, RNA Expression, Standard Deviation, Western Blot, Cell Culture, Imaging, Software, Plasmid Preparation, Transfection