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  • 92
    Thermo Fisher avidin
    Avidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 912 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin/product/Thermo Fisher
    Average 92 stars, based on 912 article reviews
    Price from $9.99 to $1999.99
    avidin - by Bioz Stars, 2020-08
    92/100 stars
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    94
    Vector Laboratories avidin
    ADS delivers antigen to the EBV B–cell surface at high concentration. ( a ) The b-PWM has preferential affinity for disulfide-bonded Ig-like molecules such as surface Ig on the EBV B cell. The b-PWM is then bound to avidin, which can bind up to 3 molecules of biotinylated antigen. ( b ) The PWM-based ADS is completely internalized in 6 hours. Priess EBV B cells were incubated sequentially on ice with each component of the ADS (b-PWM, <t>avidin-D,</t> biotinylated antigen [TT]) and then incubated in culture medium at 37°C for 0, 1, 3, or 6 hours and removed for analysis of surface TT by flow cytometry, using rabbit anti-TT antiserum and FITC goat anti-rabbit Ig. Compared with background (solid line), surface expression was high at 0 hours (long dashes) but had diminished by 1 (dashes broken by dots) and 3 hours (short dashes). ADS-delivered antigen was completely internalized by 6 hours (dotted line).
    Avidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin/product/Vector Laboratories
    Average 94 stars, based on 935 article reviews
    Price from $9.99 to $1999.99
    avidin - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    94
    BioLegend hrp avidin
    ADS delivers antigen to the EBV B–cell surface at high concentration. ( a ) The b-PWM has preferential affinity for disulfide-bonded Ig-like molecules such as surface Ig on the EBV B cell. The b-PWM is then bound to avidin, which can bind up to 3 molecules of biotinylated antigen. ( b ) The PWM-based ADS is completely internalized in 6 hours. Priess EBV B cells were incubated sequentially on ice with each component of the ADS (b-PWM, <t>avidin-D,</t> biotinylated antigen [TT]) and then incubated in culture medium at 37°C for 0, 1, 3, or 6 hours and removed for analysis of surface TT by flow cytometry, using rabbit anti-TT antiserum and FITC goat anti-rabbit Ig. Compared with background (solid line), surface expression was high at 0 hours (long dashes) but had diminished by 1 (dashes broken by dots) and 3 hours (short dashes). ADS-delivered antigen was completely internalized by 6 hours (dotted line).
    Hrp Avidin, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp avidin/product/BioLegend
    Average 94 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    hrp avidin - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    94
    Bio-Rad avidin hrp
    AADA hydrolyzes soluble and insoluble carboxylesters. Twenty micrograms of membrane protein prepared from McA cells stably transfected with an empty pCIneo vector, FLAG-tagged mouse AADA, or human TGH cDNAs were incubated with pNP-acetate (A) or 4-MUH (B). The release of pNP was monitored at 405 nm and that of 4-umbelliferyl at 460 nm. The results are presented as an average from triplicate measurements. C: Hydrolysis of microsomal lipids. Cells were incubated with radiolabeled oleic acid, and microsomes were isolated and incubated for 4 h as described in Materials and Methods. Lipids were extracted and resolved on TLC plates, and radioactivity was determined by scintillation counting. Data (mean ± SD) from three separate experiments performed in triplicate are presented with radioactivity in DG in pNeo microsomes at time 0 set as 100%. Equal amounts of microsomal protein were used for the assay. The actual starting dpm in microsomal lipid fractions at time 0 were 22,000–25,000 for PC, 12,000–18,000 for TG, and 1,500–2,300 for DG. D: Thirty micrograms of total cellular membrane proteins from McA cells stably transfected with pCI-neo, A13, A23, and human TGH were either not treated (pNeo-NT) or treated with FP-biotin, resolved in SDS-PAGE, and transferred to nitrocellulose membrane, and <t>biotinylated</t> proteins were visualized with <t>streptavidin-HRP.</t> Asterisks indicate other potential endogenous serine hydrolases present in McA cells.
    Avidin Hrp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin hrp/product/Bio-Rad
    Average 94 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    avidin hrp - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    ADS delivers antigen to the EBV B–cell surface at high concentration. ( a ) The b-PWM has preferential affinity for disulfide-bonded Ig-like molecules such as surface Ig on the EBV B cell. The b-PWM is then bound to avidin, which can bind up to 3 molecules of biotinylated antigen. ( b ) The PWM-based ADS is completely internalized in 6 hours. Priess EBV B cells were incubated sequentially on ice with each component of the ADS (b-PWM, avidin-D, biotinylated antigen [TT]) and then incubated in culture medium at 37°C for 0, 1, 3, or 6 hours and removed for analysis of surface TT by flow cytometry, using rabbit anti-TT antiserum and FITC goat anti-rabbit Ig. Compared with background (solid line), surface expression was high at 0 hours (long dashes) but had diminished by 1 (dashes broken by dots) and 3 hours (short dashes). ADS-delivered antigen was completely internalized by 6 hours (dotted line).

    Journal: Journal of Clinical Investigation

    Article Title: Naturally processed and presented epitopes of the islet cell autoantigen IA-2 eluted from HLA-DR4

    doi:

    Figure Lengend Snippet: ADS delivers antigen to the EBV B–cell surface at high concentration. ( a ) The b-PWM has preferential affinity for disulfide-bonded Ig-like molecules such as surface Ig on the EBV B cell. The b-PWM is then bound to avidin, which can bind up to 3 molecules of biotinylated antigen. ( b ) The PWM-based ADS is completely internalized in 6 hours. Priess EBV B cells were incubated sequentially on ice with each component of the ADS (b-PWM, avidin-D, biotinylated antigen [TT]) and then incubated in culture medium at 37°C for 0, 1, 3, or 6 hours and removed for analysis of surface TT by flow cytometry, using rabbit anti-TT antiserum and FITC goat anti-rabbit Ig. Compared with background (solid line), surface expression was high at 0 hours (long dashes) but had diminished by 1 (dashes broken by dots) and 3 hours (short dashes). ADS-delivered antigen was completely internalized by 6 hours (dotted line).

    Article Snippet: Cells were then pulsed sequentially on ice for 30 minutes with each component of the ADS at optimal concentrations, comprising b-PWM (1 μg/mL; Sigma Chemical Co., Poole, United Kingdom), avidin-D (2 mg/mL; Vector Laboratories), and recombinant b-IA-2ic (20 μg/mL), with 2 washing steps with excess cold HBSS between each pulse.

    Techniques: Concentration Assay, Avidin-Biotin Assay, Incubation, Flow Cytometry, Cytometry, Expressing

    AADA hydrolyzes soluble and insoluble carboxylesters. Twenty micrograms of membrane protein prepared from McA cells stably transfected with an empty pCIneo vector, FLAG-tagged mouse AADA, or human TGH cDNAs were incubated with pNP-acetate (A) or 4-MUH (B). The release of pNP was monitored at 405 nm and that of 4-umbelliferyl at 460 nm. The results are presented as an average from triplicate measurements. C: Hydrolysis of microsomal lipids. Cells were incubated with radiolabeled oleic acid, and microsomes were isolated and incubated for 4 h as described in Materials and Methods. Lipids were extracted and resolved on TLC plates, and radioactivity was determined by scintillation counting. Data (mean ± SD) from three separate experiments performed in triplicate are presented with radioactivity in DG in pNeo microsomes at time 0 set as 100%. Equal amounts of microsomal protein were used for the assay. The actual starting dpm in microsomal lipid fractions at time 0 were 22,000–25,000 for PC, 12,000–18,000 for TG, and 1,500–2,300 for DG. D: Thirty micrograms of total cellular membrane proteins from McA cells stably transfected with pCI-neo, A13, A23, and human TGH were either not treated (pNeo-NT) or treated with FP-biotin, resolved in SDS-PAGE, and transferred to nitrocellulose membrane, and biotinylated proteins were visualized with streptavidin-HRP. Asterisks indicate other potential endogenous serine hydrolases present in McA cells.

    Journal: Journal of Lipid Research

    Article Title: Arylacetamide deacetylase attenuates fatty-acid-induced triacylglycerol accumulation in rat hepatoma cells

    doi: 10.1194/jlr.M000596

    Figure Lengend Snippet: AADA hydrolyzes soluble and insoluble carboxylesters. Twenty micrograms of membrane protein prepared from McA cells stably transfected with an empty pCIneo vector, FLAG-tagged mouse AADA, or human TGH cDNAs were incubated with pNP-acetate (A) or 4-MUH (B). The release of pNP was monitored at 405 nm and that of 4-umbelliferyl at 460 nm. The results are presented as an average from triplicate measurements. C: Hydrolysis of microsomal lipids. Cells were incubated with radiolabeled oleic acid, and microsomes were isolated and incubated for 4 h as described in Materials and Methods. Lipids were extracted and resolved on TLC plates, and radioactivity was determined by scintillation counting. Data (mean ± SD) from three separate experiments performed in triplicate are presented with radioactivity in DG in pNeo microsomes at time 0 set as 100%. Equal amounts of microsomal protein were used for the assay. The actual starting dpm in microsomal lipid fractions at time 0 were 22,000–25,000 for PC, 12,000–18,000 for TG, and 1,500–2,300 for DG. D: Thirty micrograms of total cellular membrane proteins from McA cells stably transfected with pCI-neo, A13, A23, and human TGH were either not treated (pNeo-NT) or treated with FP-biotin, resolved in SDS-PAGE, and transferred to nitrocellulose membrane, and biotinylated proteins were visualized with streptavidin-HRP. Asterisks indicate other potential endogenous serine hydrolases present in McA cells.

    Article Snippet: Following transfer to nitrocellulose, biotinylated proteins were visualized by probing with avidin-HRP and enhanced chemiluminescence (ECL).

    Techniques: Stable Transfection, Transfection, Plasmid Preparation, Incubation, Isolation, Thin Layer Chromatography, Radioactivity, SDS Page