avidin-biotin peroxidase complex Search Results


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  • 99
    Vector Laboratories avidin biotin peroxidase complex
    Light microscopic finding in the livers of experimentally H5N8B-infected ducks. (A, B) Seropositive mallard, H5N8B-infected, clinically normal, 34 dpi, liver. (A) No obvious findings. (B) Lack of immunohistochemically-detectable hepatocellular influenza A virus matrixprotein antigen. (C, D) Pekin duck, contact animal, died 4 days post contact, liver. (C) Marked hypereosinophilia, hepatocellular vacuolation, membraneous rupture and nuclear pyknosis, karyorrhexis and lysis interpreted as severe, acute, coalescing to diffuse necrotizing hepatitis. (D) Immunohistochemistry reveals coalescing intrahepatocytic, intracytoplasmic and intranuclear influenza A virus matrix protein. (A, C) Hematoxylin-eosin, (B, D) Immunohistochemistry using the <t>avidin-biotin-peroxidase-complex</t> <t>method</t> with a monoclonal antibody against influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (redbrown) and hematoxylin counterstain (blue). (A, C) bars = 20 μm. (B, D) bars = 50 μm.
    Avidin Biotin Peroxidase Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 13175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore avidin biotin peroxidase complex
    Light microscopic finding in the livers of experimentally H5N8B-infected ducks. (A, B) Seropositive mallard, H5N8B-infected, clinically normal, 34 dpi, liver. (A) No obvious findings. (B) Lack of immunohistochemically-detectable hepatocellular influenza A virus matrixprotein antigen. (C, D) Pekin duck, contact animal, died 4 days post contact, liver. (C) Marked hypereosinophilia, hepatocellular vacuolation, membraneous rupture and nuclear pyknosis, karyorrhexis and lysis interpreted as severe, acute, coalescing to diffuse necrotizing hepatitis. (D) Immunohistochemistry reveals coalescing intrahepatocytic, intracytoplasmic and intranuclear influenza A virus matrix protein. (A, C) Hematoxylin-eosin, (B, D) Immunohistochemistry using the <t>avidin-biotin-peroxidase-complex</t> <t>method</t> with a monoclonal antibody against influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (redbrown) and hematoxylin counterstain (blue). (A, C) bars = 20 μm. (B, D) bars = 50 μm.
    Avidin Biotin Peroxidase Complex, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 361 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies avidin biotin peroxidase complex
    Light microscopic finding in the livers of experimentally H5N8B-infected ducks. (A, B) Seropositive mallard, H5N8B-infected, clinically normal, 34 dpi, liver. (A) No obvious findings. (B) Lack of immunohistochemically-detectable hepatocellular influenza A virus matrixprotein antigen. (C, D) Pekin duck, contact animal, died 4 days post contact, liver. (C) Marked hypereosinophilia, hepatocellular vacuolation, membraneous rupture and nuclear pyknosis, karyorrhexis and lysis interpreted as severe, acute, coalescing to diffuse necrotizing hepatitis. (D) Immunohistochemistry reveals coalescing intrahepatocytic, intracytoplasmic and intranuclear influenza A virus matrix protein. (A, C) Hematoxylin-eosin, (B, D) Immunohistochemistry using the <t>avidin-biotin-peroxidase-complex</t> <t>method</t> with a monoclonal antibody against influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (redbrown) and hematoxylin counterstain (blue). (A, C) bars = 20 μm. (B, D) bars = 50 μm.
    Avidin Biotin Peroxidase Complex, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Vector Laboratories avidin biotinylated horseradish peroxidase complex
    Binding of gD:Fc to CHO cells expressing nectin-1α (FVB/N form). CHO-K1 cells transfected with nectin-1α-expressing plasmid (pDS101) or control plasmid (pcDNA3) were replated in 96-well plates and exposed to purified HSV-1 gD:Fc that had been preincubated with anti-gD MAbs (or control anti-Myc MAb) or buffer alone. After 30 min, the cells were washed, fixed with formaldehyde-glutaraldehyde, and incubated with <t>biotinylated</t> anti-rabbit Fc antibody followed by streptavidin-conjugated horseradish peroxidase. Peroxidase activity was assayed as a measure of gD:Fc binding to the cell surface. The results shown are means of three determinations with standard deviations indicated. OD 370 , optical density at 370 nm.
    Avidin Biotinylated Horseradish Peroxidase Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 2010 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    GE Healthcare streptavidin biotinylated horseradish peroxidase complex
    Factor C as a surface protein on hemocytes. ( A ) The fixed hemocytes were stained with 1 μg/ml of a polyclonal antibody against factor C followed by FITC-labeled secondary antibody (solid line). The gray line shows cytometric analysis with the first polyclonal antibody pretreated with an excess amount of the purified factor C, and the dotted line shows the negative control without the first antibody. ( B ) Inhibition of the LPS-induced exocytosis by mAb 2C12. Hemocytes were preincubated with mAb 2C12 at 0.1 μg/ml (bar 2), 0.2 μg/ml (bar 3), and 0.5 μg/ml (bar 4) or without mAb 2C12 (bar 1) at 23°C for 1 h. Then, exocytosis was induced by LPS at 1.0 μg/ml. ( C ) <t>Biotinylated</t> surface antigens were immunoprecipitated by mAb 2C12 and subjected to SDS/PAGE under reducing conditions. The factor C-like cell surface antigen was visualized by <t>streptavidin-biotinylated</t> horseradish peroxidase. The arrowhead shows the apparent molecular mass.
    Streptavidin Biotinylated Horseradish Peroxidase Complex, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher avidin biotin peroxidase complex
    Factor C as a surface protein on hemocytes. ( A ) The fixed hemocytes were stained with 1 μg/ml of a polyclonal antibody against factor C followed by FITC-labeled secondary antibody (solid line). The gray line shows cytometric analysis with the first polyclonal antibody pretreated with an excess amount of the purified factor C, and the dotted line shows the negative control without the first antibody. ( B ) Inhibition of the LPS-induced exocytosis by mAb 2C12. Hemocytes were preincubated with mAb 2C12 at 0.1 μg/ml (bar 2), 0.2 μg/ml (bar 3), and 0.5 μg/ml (bar 4) or without mAb 2C12 (bar 1) at 23°C for 1 h. Then, exocytosis was induced by LPS at 1.0 μg/ml. ( C ) <t>Biotinylated</t> surface antigens were immunoprecipitated by mAb 2C12 and subjected to SDS/PAGE under reducing conditions. The factor C-like cell surface antigen was visualized by <t>streptavidin-biotinylated</t> horseradish peroxidase. The arrowhead shows the apparent molecular mass.
    Avidin Biotin Peroxidase Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 349 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ventana Medical avidin biotin peroxidase complex
    Factor C as a surface protein on hemocytes. ( A ) The fixed hemocytes were stained with 1 μg/ml of a polyclonal antibody against factor C followed by FITC-labeled secondary antibody (solid line). The gray line shows cytometric analysis with the first polyclonal antibody pretreated with an excess amount of the purified factor C, and the dotted line shows the negative control without the first antibody. ( B ) Inhibition of the LPS-induced exocytosis by mAb 2C12. Hemocytes were preincubated with mAb 2C12 at 0.1 μg/ml (bar 2), 0.2 μg/ml (bar 3), and 0.5 μg/ml (bar 4) or without mAb 2C12 (bar 1) at 23°C for 1 h. Then, exocytosis was induced by LPS at 1.0 μg/ml. ( C ) <t>Biotinylated</t> surface antigens were immunoprecipitated by mAb 2C12 and subjected to SDS/PAGE under reducing conditions. The factor C-like cell surface antigen was visualized by <t>streptavidin-biotinylated</t> horseradish peroxidase. The arrowhead shows the apparent molecular mass.
    Avidin Biotin Peroxidase Complex, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 94/100, based on 270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Vector Laboratories avidin biotin peroxidase complex solution
    Factor C as a surface protein on hemocytes. ( A ) The fixed hemocytes were stained with 1 μg/ml of a polyclonal antibody against factor C followed by FITC-labeled secondary antibody (solid line). The gray line shows cytometric analysis with the first polyclonal antibody pretreated with an excess amount of the purified factor C, and the dotted line shows the negative control without the first antibody. ( B ) Inhibition of the LPS-induced exocytosis by mAb 2C12. Hemocytes were preincubated with mAb 2C12 at 0.1 μg/ml (bar 2), 0.2 μg/ml (bar 3), and 0.5 μg/ml (bar 4) or without mAb 2C12 (bar 1) at 23°C for 1 h. Then, exocytosis was induced by LPS at 1.0 μg/ml. ( C ) <t>Biotinylated</t> surface antigens were immunoprecipitated by mAb 2C12 and subjected to SDS/PAGE under reducing conditions. The factor C-like cell surface antigen was visualized by <t>streptavidin-biotinylated</t> horseradish peroxidase. The arrowhead shows the apparent molecular mass.
    Avidin Biotin Peroxidase Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories avidin biotin peroxidase complex technique
    IHC detection of DCs in skin from the heel-bulb of swine. (a) Skin from control animals or Ad5-IFN-treated swine was harvested 1 dpi (0 dpc; panels 1, 3, 5, and 7) and 2 dpi (1 dpc; panels 2, 4, 6, and 8) after Ad5 treatment, and positive cells were detected by IHC using the <t>avidin-biotin-peroxidase</t> <t>complex</t> <t>technique</t> and developed with 3,3′-diaminobenzidine, which gives a brown product where primary antibody binds (dark brown); sections were counterstained with Harry's hematoxylin. Arrows indicate the positive staining for DCs in control animals; these cells appeared small and localized in the stratum basale of the epidermis. Arrowheads indicate the positive staining of DCs in treated animals; these cells appeared larger than positive cells in control animals, showed dendrites, and were localized along the stratum spinosum. (b) Double-IFA detection of CD207/Langerin (red) and CD1 (green) in the epidermis of the heel-bulb. Nuclei were stained with DAPI (blue). (c) Quantification of the number of positive cells in the tissue sections was performed as described in Materials and Methods. The number of positive cells is significantly higher in IFN-treated animals than in the control animals (*, P
    Avidin Biotin Peroxidase Complex Technique, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology avidin biotin peroxidase complex
    IHC detection of DCs in skin from the heel-bulb of swine. (a) Skin from control animals or Ad5-IFN-treated swine was harvested 1 dpi (0 dpc; panels 1, 3, 5, and 7) and 2 dpi (1 dpc; panels 2, 4, 6, and 8) after Ad5 treatment, and positive cells were detected by IHC using the <t>avidin-biotin-peroxidase</t> <t>complex</t> <t>technique</t> and developed with 3,3′-diaminobenzidine, which gives a brown product where primary antibody binds (dark brown); sections were counterstained with Harry's hematoxylin. Arrows indicate the positive staining for DCs in control animals; these cells appeared small and localized in the stratum basale of the epidermis. Arrowheads indicate the positive staining of DCs in treated animals; these cells appeared larger than positive cells in control animals, showed dendrites, and were localized along the stratum spinosum. (b) Double-IFA detection of CD207/Langerin (red) and CD1 (green) in the epidermis of the heel-bulb. Nuclei were stained with DAPI (blue). (c) Quantification of the number of positive cells in the tissue sections was performed as described in Materials and Methods. The number of positive cells is significantly higher in IFN-treated animals than in the control animals (*, P
    Avidin Biotin Peroxidase Complex, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies avidin biotin peroxidase complex technique
    IHC detection of DCs in skin from the heel-bulb of swine. (a) Skin from control animals or Ad5-IFN-treated swine was harvested 1 dpi (0 dpc; panels 1, 3, 5, and 7) and 2 dpi (1 dpc; panels 2, 4, 6, and 8) after Ad5 treatment, and positive cells were detected by IHC using the <t>avidin-biotin-peroxidase</t> <t>complex</t> <t>technique</t> and developed with 3,3′-diaminobenzidine, which gives a brown product where primary antibody binds (dark brown); sections were counterstained with Harry's hematoxylin. Arrows indicate the positive staining for DCs in control animals; these cells appeared small and localized in the stratum basale of the epidermis. Arrowheads indicate the positive staining of DCs in treated animals; these cells appeared larger than positive cells in control animals, showed dendrites, and were localized along the stratum spinosum. (b) Double-IFA detection of CD207/Langerin (red) and CD1 (green) in the epidermis of the heel-bulb. Nuclei were stained with DAPI (blue). (c) Quantification of the number of positive cells in the tissue sections was performed as described in Materials and Methods. The number of positive cells is significantly higher in IFN-treated animals than in the control animals (*, P
    Avidin Biotin Peroxidase Complex Technique, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies avidin biotinylated horseradish peroxidase complex
    IHC detection of DCs in skin from the heel-bulb of swine. (a) Skin from control animals or Ad5-IFN-treated swine was harvested 1 dpi (0 dpc; panels 1, 3, 5, and 7) and 2 dpi (1 dpc; panels 2, 4, 6, and 8) after Ad5 treatment, and positive cells were detected by IHC using the <t>avidin-biotin-peroxidase</t> <t>complex</t> <t>technique</t> and developed with 3,3′-diaminobenzidine, which gives a brown product where primary antibody binds (dark brown); sections were counterstained with Harry's hematoxylin. Arrows indicate the positive staining for DCs in control animals; these cells appeared small and localized in the stratum basale of the epidermis. Arrowheads indicate the positive staining of DCs in treated animals; these cells appeared larger than positive cells in control animals, showed dendrites, and were localized along the stratum spinosum. (b) Double-IFA detection of CD207/Langerin (red) and CD1 (green) in the epidermis of the heel-bulb. Nuclei were stained with DAPI (blue). (c) Quantification of the number of positive cells in the tissue sections was performed as described in Materials and Methods. The number of positive cells is significantly higher in IFN-treated animals than in the control animals (*, P
    Avidin Biotinylated Horseradish Peroxidase Complex, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies avidin biotin peroxidase complex method
    Gastric mucosal defect, dog. a Absence of lining epithelium, presence of bleeding ulcer, and mononuclear inflammatory cell infiltration in periglandular connective tissue in the control group. b Regenerated lining epithelium, hypervascularization of underlying tissue, and presence of mononuclear inflammatory cell infiltration in periglandular connective tissue (H E × 200). c Poorly observed bluish stained connective tissue in mucosa of control ulcer. d Fine fibers of connective tissue below regenerated epithelium in the gastric ulcer with AM graft (Masson’s trichrome × 200). e Negative staining of collagen IA1 in the exposed granulation tissue in the control ulcer and f negative staining of subepithelial connective tissue in the gastric ulcer with AM graft <t>(avidin–biotin–peroxidase</t> <t>complex</t> <t>method,</t> hematoxylin counterstain × 400)
    Avidin Biotin Peroxidase Complex Method, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies avidin biotin horseradish peroxidase complex
    Gastric mucosal defect, dog. a Absence of lining epithelium, presence of bleeding ulcer, and mononuclear inflammatory cell infiltration in periglandular connective tissue in the control group. b Regenerated lining epithelium, hypervascularization of underlying tissue, and presence of mononuclear inflammatory cell infiltration in periglandular connective tissue (H E × 200). c Poorly observed bluish stained connective tissue in mucosa of control ulcer. d Fine fibers of connective tissue below regenerated epithelium in the gastric ulcer with AM graft (Masson’s trichrome × 200). e Negative staining of collagen IA1 in the exposed granulation tissue in the control ulcer and f negative staining of subepithelial connective tissue in the gastric ulcer with AM graft <t>(avidin–biotin–peroxidase</t> <t>complex</t> <t>method,</t> hematoxylin counterstain × 400)
    Avidin Biotin Horseradish Peroxidase Complex, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Light microscopic finding in the livers of experimentally H5N8B-infected ducks. (A, B) Seropositive mallard, H5N8B-infected, clinically normal, 34 dpi, liver. (A) No obvious findings. (B) Lack of immunohistochemically-detectable hepatocellular influenza A virus matrixprotein antigen. (C, D) Pekin duck, contact animal, died 4 days post contact, liver. (C) Marked hypereosinophilia, hepatocellular vacuolation, membraneous rupture and nuclear pyknosis, karyorrhexis and lysis interpreted as severe, acute, coalescing to diffuse necrotizing hepatitis. (D) Immunohistochemistry reveals coalescing intrahepatocytic, intracytoplasmic and intranuclear influenza A virus matrix protein. (A, C) Hematoxylin-eosin, (B, D) Immunohistochemistry using the avidin-biotin-peroxidase-complex method with a monoclonal antibody against influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (redbrown) and hematoxylin counterstain (blue). (A, C) bars = 20 μm. (B, D) bars = 50 μm.

    Journal: Emerging Microbes & Infections

    Article Title: Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards

    doi: 10.1080/22221751.2020.1713706

    Figure Lengend Snippet: Light microscopic finding in the livers of experimentally H5N8B-infected ducks. (A, B) Seropositive mallard, H5N8B-infected, clinically normal, 34 dpi, liver. (A) No obvious findings. (B) Lack of immunohistochemically-detectable hepatocellular influenza A virus matrixprotein antigen. (C, D) Pekin duck, contact animal, died 4 days post contact, liver. (C) Marked hypereosinophilia, hepatocellular vacuolation, membraneous rupture and nuclear pyknosis, karyorrhexis and lysis interpreted as severe, acute, coalescing to diffuse necrotizing hepatitis. (D) Immunohistochemistry reveals coalescing intrahepatocytic, intracytoplasmic and intranuclear influenza A virus matrix protein. (A, C) Hematoxylin-eosin, (B, D) Immunohistochemistry using the avidin-biotin-peroxidase-complex method with a monoclonal antibody against influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (redbrown) and hematoxylin counterstain (blue). (A, C) bars = 20 μm. (B, D) bars = 50 μm.

    Article Snippet: Immunohistochemistry was employed to detect influenza A virus matrix protein using the avidin–biotin-peroxidase-complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a monoclonal antibody (mAb) directed against an epitope of the influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain [ ].

    Techniques: Infection, Lysis, Immunohistochemistry, Avidin-Biotin Assay

    Binding of gD:Fc to CHO cells expressing nectin-1α (FVB/N form). CHO-K1 cells transfected with nectin-1α-expressing plasmid (pDS101) or control plasmid (pcDNA3) were replated in 96-well plates and exposed to purified HSV-1 gD:Fc that had been preincubated with anti-gD MAbs (or control anti-Myc MAb) or buffer alone. After 30 min, the cells were washed, fixed with formaldehyde-glutaraldehyde, and incubated with biotinylated anti-rabbit Fc antibody followed by streptavidin-conjugated horseradish peroxidase. Peroxidase activity was assayed as a measure of gD:Fc binding to the cell surface. The results shown are means of three determinations with standard deviations indicated. OD 370 , optical density at 370 nm.

    Journal: Journal of Virology

    Article Title: Striking Similarity of Murine Nectin-1? to Human Nectin-1? (HveC) in Sequence and Activity as a Glycoprotein D Receptor for Alphaherpesvirus Entry

    doi:

    Figure Lengend Snippet: Binding of gD:Fc to CHO cells expressing nectin-1α (FVB/N form). CHO-K1 cells transfected with nectin-1α-expressing plasmid (pDS101) or control plasmid (pcDNA3) were replated in 96-well plates and exposed to purified HSV-1 gD:Fc that had been preincubated with anti-gD MAbs (or control anti-Myc MAb) or buffer alone. After 30 min, the cells were washed, fixed with formaldehyde-glutaraldehyde, and incubated with biotinylated anti-rabbit Fc antibody followed by streptavidin-conjugated horseradish peroxidase. Peroxidase activity was assayed as a measure of gD:Fc binding to the cell surface. The results shown are means of three determinations with standard deviations indicated. OD 370 , optical density at 370 nm.

    Article Snippet: After 1 h, the sections were washed in PBS and incubated with biotinylated goat anti-rabbit IgG for 30 min. After further washes with PBS, the sections were incubated with an avidin-biotinylated horseradish peroxidase complex for 1 h. The sections were washed in PBS and developed by the addition of Vector VIP substrate for peroxidase (Vector Laboratories) as specified by the manufacturer.

    Techniques: Binding Assay, Expressing, Transfection, Plasmid Preparation, Purification, Incubation, Activity Assay

    IHC analysis for leukocyte common antigen (CD45). Avidin-biotin-peroxidase complex method. Hematoxylin counterstain. (a) IPP of a control lamb (no. 23). The majority of cells in the follicles (F) are CD45 positive. Bar, 480 μm. (b) IPP of lamb no. 16 infected with E. ovinoidalis . The atrophic ileal follicles (F) contain scattered CD45-positive cells. Bar, 490 μm.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: Lymphocyte Depletion in Ileal Peyer's Patch Follicles in Lambs Infected with Eimeria ovinoidalis

    doi: 10.1128/CDLI.9.1.83-91.2002

    Figure Lengend Snippet: IHC analysis for leukocyte common antigen (CD45). Avidin-biotin-peroxidase complex method. Hematoxylin counterstain. (a) IPP of a control lamb (no. 23). The majority of cells in the follicles (F) are CD45 positive. Bar, 480 μm. (b) IPP of lamb no. 16 infected with E. ovinoidalis . The atrophic ileal follicles (F) contain scattered CD45-positive cells. Bar, 490 μm.

    Article Snippet: Subsequently, inhibition of endogenous peroxidase was carried out by treatment with 0.03% H2 O2 in 50 mM imidazole buffer (pH 7.4) for 10 min. Avidin-biotin-horseradish peroxidase complex (Vector Laboratories) was diluted 1:200 in phosphate-buffered saline and incubated for 30 min. Peroxidase reactivity was visualized by treatment for 2 min with a metal-enhanced diaminobenzidine substrate (Pierce Chemical Company, Rockford, Ill.) diluted 1:10 in peroxide substrate buffer.

    Techniques: Immunohistochemistry, Avidin-Biotin Assay, Infection

    IHC analysis for B-cell marker CD21. Avidin-biotin-peroxidase complex method. Hematoxylin counterstain. (a) IPP of a control lamb (no. 20). Numerous positive cells are present in the follicles. Bar, 175 μm. (b) IPP of an infected lamb (no. 16). B cells are scarce in the lymphoid follicles. Bar, 175 μm.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: Lymphocyte Depletion in Ileal Peyer's Patch Follicles in Lambs Infected with Eimeria ovinoidalis

    doi: 10.1128/CDLI.9.1.83-91.2002

    Figure Lengend Snippet: IHC analysis for B-cell marker CD21. Avidin-biotin-peroxidase complex method. Hematoxylin counterstain. (a) IPP of a control lamb (no. 20). Numerous positive cells are present in the follicles. Bar, 175 μm. (b) IPP of an infected lamb (no. 16). B cells are scarce in the lymphoid follicles. Bar, 175 μm.

    Article Snippet: Subsequently, inhibition of endogenous peroxidase was carried out by treatment with 0.03% H2 O2 in 50 mM imidazole buffer (pH 7.4) for 10 min. Avidin-biotin-horseradish peroxidase complex (Vector Laboratories) was diluted 1:200 in phosphate-buffered saline and incubated for 30 min. Peroxidase reactivity was visualized by treatment for 2 min with a metal-enhanced diaminobenzidine substrate (Pierce Chemical Company, Rockford, Ill.) diluted 1:10 in peroxide substrate buffer.

    Techniques: Immunohistochemistry, Marker, Avidin-Biotin Assay, Infection

    IHC analysis for proliferation marker Ki-67. Avidin-biotin-peroxidase complex method. Hematoxylin counterstain. (a) IPP of a control lamb (no. 20). Intense staining of follicle lymphocytes is present. Bar, 480 μm. (b) IPP of an infected lamb (no. 16). Few cells in the follicles (F) are positive. Prominent follicle atrophy is present. Bar, 480 μm.

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: Lymphocyte Depletion in Ileal Peyer's Patch Follicles in Lambs Infected with Eimeria ovinoidalis

    doi: 10.1128/CDLI.9.1.83-91.2002

    Figure Lengend Snippet: IHC analysis for proliferation marker Ki-67. Avidin-biotin-peroxidase complex method. Hematoxylin counterstain. (a) IPP of a control lamb (no. 20). Intense staining of follicle lymphocytes is present. Bar, 480 μm. (b) IPP of an infected lamb (no. 16). Few cells in the follicles (F) are positive. Prominent follicle atrophy is present. Bar, 480 μm.

    Article Snippet: Subsequently, inhibition of endogenous peroxidase was carried out by treatment with 0.03% H2 O2 in 50 mM imidazole buffer (pH 7.4) for 10 min. Avidin-biotin-horseradish peroxidase complex (Vector Laboratories) was diluted 1:200 in phosphate-buffered saline and incubated for 30 min. Peroxidase reactivity was visualized by treatment for 2 min with a metal-enhanced diaminobenzidine substrate (Pierce Chemical Company, Rockford, Ill.) diluted 1:10 in peroxide substrate buffer.

    Techniques: Immunohistochemistry, Marker, Avidin-Biotin Assay, Staining, Infection

    Differences in serum gp70 expression and glomerular pathology induced after injection of purified 12H5.1 IgG3 in NZW, (BALB/c × MRL/+)F 1 , and B6 mice. (a) Results of Western blotting assays showing the expression of serum gp70 in three different strains of mice. Sera were diluted 1:20 into SDS sample buffer without a reducing reagent and boiled for 5 min; 10 μl of each boiled mixture was loaded into a well of 7.5% polyacrylamide gel. Plasma from a 4 month-old female MRL/ lpr ), NZW mice expressed a high level of serum gp85 (gp70 plus p15E), gp70, and a degradation product gp45 (arrowheads), while their expression in B6 mice was low. (BALB/c × MRL/+)F 1 mice (F 1 ) expressed an intermediate level of serum gp70. Mr, biotinylated markers, with positions indicated in kilodaltons at the left. (b to d) Representative photomicrographs taken from kidney sections of NZW (b), (BALB/c × MRL/+)F 1 (c), and B6 (d) mice injected with purified anti-gp70 IgG3, 12H5.1; hematoxylin and eosin staining, ×300. Note apparent thickening of the capillary walls (arrowheads) and inflammatory cell infiltration (arrow) in panel b and marked increase in glomerular cellularity and evident neutrophilic infiltration in panel c.

    Journal: Journal of Virology

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice

    doi:

    Figure Lengend Snippet: Differences in serum gp70 expression and glomerular pathology induced after injection of purified 12H5.1 IgG3 in NZW, (BALB/c × MRL/+)F 1 , and B6 mice. (a) Results of Western blotting assays showing the expression of serum gp70 in three different strains of mice. Sera were diluted 1:20 into SDS sample buffer without a reducing reagent and boiled for 5 min; 10 μl of each boiled mixture was loaded into a well of 7.5% polyacrylamide gel. Plasma from a 4 month-old female MRL/ lpr ), NZW mice expressed a high level of serum gp85 (gp70 plus p15E), gp70, and a degradation product gp45 (arrowheads), while their expression in B6 mice was low. (BALB/c × MRL/+)F 1 mice (F 1 ) expressed an intermediate level of serum gp70. Mr, biotinylated markers, with positions indicated in kilodaltons at the left. (b to d) Representative photomicrographs taken from kidney sections of NZW (b), (BALB/c × MRL/+)F 1 (c), and B6 (d) mice injected with purified anti-gp70 IgG3, 12H5.1; hematoxylin and eosin staining, ×300. Note apparent thickening of the capillary walls (arrowheads) and inflammatory cell infiltration (arrow) in panel b and marked increase in glomerular cellularity and evident neutrophilic infiltration in panel c.

    Article Snippet: Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ).

    Techniques: Expressing, Injection, Purification, Mouse Assay, Western Blot, Staining

    Representative kidney pathology of hybridoma-transplanted mice. (a) (BALB/c × MRL/+)F 1 mouse transplanted with 8.653 fusion partner cells. PAS staining, ×70. (b) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. PAS staining, ×70. Note the extreme expansion of the glomeruli compared to those in panel a, which shows normal glomeruli at the same magnification. Sizes of tubules and of the nuclei of tubular epithelial cells are not different in panels a and b, but glomeruli are markedly enlarged in panel b. Granular deposition of fibrin in the affected glomeruli was also shown when phosphotungstenic acid-hematoxylin staining was applied (not shown). (c) Immunofluorescence staining with FITC-conjugated anti-mouse IgG of a fresh-frozen section taken from a representative (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. Use of FITC-conjugated anti-mouse C3 resulted in a similar pattern of staining. (d) Electron micrograph showing an affected glomerulus of a representative (BALB/c × MRL/+)F 1 ) was biotinylated to detect the presence of xenotropic viral env gene products. (g) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1 showing typical wire loop lesions. PAS staining, ×175. (h) Electron micrograph of the kidney from a (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1. Bar = 2 μm. Note the dense subendothelial deposits consistent with light microscopic wire loops along the basement membrane. (i) SCID mouse transplanted with hybridoma cells 37C4.1. PAS staining, ×140. (j) Dense linear deposition of mouse C3 in a representative glomerulus from a SCID mouse transplanted with hybridoma 37C4.1. Similar deposition of mouse IgG and xenotropic viral gp70 in the affected glomeruli was also demonstrated in fresh-frozen sections of the transplanted SCID mice. (k and l) (BALB/c × MRL/+)F 1 mice transplanted with one clone of hybridomas 51D1.1 and 59C4.1, respectively. PAS staining, ×140. Note PAS-positive deposition and expansion of the mesangial areas in panel k and cell proliferation (arrowheads) and occlusive changes (arrow) of capillaries in panel l. Lesions similar to those in panel l were observed in the mice transplanted with hybridoma 60A5.1 (not shown).

    Journal: Journal of Virology

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice

    doi:

    Figure Lengend Snippet: Representative kidney pathology of hybridoma-transplanted mice. (a) (BALB/c × MRL/+)F 1 mouse transplanted with 8.653 fusion partner cells. PAS staining, ×70. (b) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. PAS staining, ×70. Note the extreme expansion of the glomeruli compared to those in panel a, which shows normal glomeruli at the same magnification. Sizes of tubules and of the nuclei of tubular epithelial cells are not different in panels a and b, but glomeruli are markedly enlarged in panel b. Granular deposition of fibrin in the affected glomeruli was also shown when phosphotungstenic acid-hematoxylin staining was applied (not shown). (c) Immunofluorescence staining with FITC-conjugated anti-mouse IgG of a fresh-frozen section taken from a representative (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. Use of FITC-conjugated anti-mouse C3 resulted in a similar pattern of staining. (d) Electron micrograph showing an affected glomerulus of a representative (BALB/c × MRL/+)F 1 ) was biotinylated to detect the presence of xenotropic viral env gene products. (g) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1 showing typical wire loop lesions. PAS staining, ×175. (h) Electron micrograph of the kidney from a (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1. Bar = 2 μm. Note the dense subendothelial deposits consistent with light microscopic wire loops along the basement membrane. (i) SCID mouse transplanted with hybridoma cells 37C4.1. PAS staining, ×140. (j) Dense linear deposition of mouse C3 in a representative glomerulus from a SCID mouse transplanted with hybridoma 37C4.1. Similar deposition of mouse IgG and xenotropic viral gp70 in the affected glomeruli was also demonstrated in fresh-frozen sections of the transplanted SCID mice. (k and l) (BALB/c × MRL/+)F 1 mice transplanted with one clone of hybridomas 51D1.1 and 59C4.1, respectively. PAS staining, ×140. Note PAS-positive deposition and expansion of the mesangial areas in panel k and cell proliferation (arrowheads) and occlusive changes (arrow) of capillaries in panel l. Lesions similar to those in panel l were observed in the mice transplanted with hybridoma 60A5.1 (not shown).

    Article Snippet: Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ).

    Techniques: Mouse Assay, Staining, Immunofluorescence

    Factor C as a surface protein on hemocytes. ( A ) The fixed hemocytes were stained with 1 μg/ml of a polyclonal antibody against factor C followed by FITC-labeled secondary antibody (solid line). The gray line shows cytometric analysis with the first polyclonal antibody pretreated with an excess amount of the purified factor C, and the dotted line shows the negative control without the first antibody. ( B ) Inhibition of the LPS-induced exocytosis by mAb 2C12. Hemocytes were preincubated with mAb 2C12 at 0.1 μg/ml (bar 2), 0.2 μg/ml (bar 3), and 0.5 μg/ml (bar 4) or without mAb 2C12 (bar 1) at 23°C for 1 h. Then, exocytosis was induced by LPS at 1.0 μg/ml. ( C ) Biotinylated surface antigens were immunoprecipitated by mAb 2C12 and subjected to SDS/PAGE under reducing conditions. The factor C-like cell surface antigen was visualized by streptavidin-biotinylated horseradish peroxidase. The arrowhead shows the apparent molecular mass.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A serine protease zymogen functions as a pattern-recognition receptor for lipopolysaccharides

    doi: 10.1073/pnas.0306904101

    Figure Lengend Snippet: Factor C as a surface protein on hemocytes. ( A ) The fixed hemocytes were stained with 1 μg/ml of a polyclonal antibody against factor C followed by FITC-labeled secondary antibody (solid line). The gray line shows cytometric analysis with the first polyclonal antibody pretreated with an excess amount of the purified factor C, and the dotted line shows the negative control without the first antibody. ( B ) Inhibition of the LPS-induced exocytosis by mAb 2C12. Hemocytes were preincubated with mAb 2C12 at 0.1 μg/ml (bar 2), 0.2 μg/ml (bar 3), and 0.5 μg/ml (bar 4) or without mAb 2C12 (bar 1) at 23°C for 1 h. Then, exocytosis was induced by LPS at 1.0 μg/ml. ( C ) Biotinylated surface antigens were immunoprecipitated by mAb 2C12 and subjected to SDS/PAGE under reducing conditions. The factor C-like cell surface antigen was visualized by streptavidin-biotinylated horseradish peroxidase. The arrowhead shows the apparent molecular mass.

    Article Snippet: After blocking, the antigens were visualized by streptavidin-biotinylated horseradish-peroxidase complex (Amersham Pharmacia).

    Techniques: Staining, Labeling, Purification, Negative Control, Inhibition, Immunoprecipitation, SDS Page

    IHC detection of DCs in skin from the heel-bulb of swine. (a) Skin from control animals or Ad5-IFN-treated swine was harvested 1 dpi (0 dpc; panels 1, 3, 5, and 7) and 2 dpi (1 dpc; panels 2, 4, 6, and 8) after Ad5 treatment, and positive cells were detected by IHC using the avidin-biotin-peroxidase complex technique and developed with 3,3′-diaminobenzidine, which gives a brown product where primary antibody binds (dark brown); sections were counterstained with Harry's hematoxylin. Arrows indicate the positive staining for DCs in control animals; these cells appeared small and localized in the stratum basale of the epidermis. Arrowheads indicate the positive staining of DCs in treated animals; these cells appeared larger than positive cells in control animals, showed dendrites, and were localized along the stratum spinosum. (b) Double-IFA detection of CD207/Langerin (red) and CD1 (green) in the epidermis of the heel-bulb. Nuclei were stained with DAPI (blue). (c) Quantification of the number of positive cells in the tissue sections was performed as described in Materials and Methods. The number of positive cells is significantly higher in IFN-treated animals than in the control animals (*, P

    Journal: Journal of Virology

    Article Title: Interferon-Induced Protection against Foot-and-Mouth Disease Virus Infection Correlates with Enhanced Tissue-Specific Innate Immune Cell Infiltration and Interferon-Stimulated Gene Expression ▿Interferon-Induced Protection against Foot-and-Mouth Disease Virus Infection Correlates with Enhanced Tissue-Specific Innate Immune Cell Infiltration and Interferon-Stimulated Gene Expression ▿ †

    doi: 10.1128/JVI.01874-09

    Figure Lengend Snippet: IHC detection of DCs in skin from the heel-bulb of swine. (a) Skin from control animals or Ad5-IFN-treated swine was harvested 1 dpi (0 dpc; panels 1, 3, 5, and 7) and 2 dpi (1 dpc; panels 2, 4, 6, and 8) after Ad5 treatment, and positive cells were detected by IHC using the avidin-biotin-peroxidase complex technique and developed with 3,3′-diaminobenzidine, which gives a brown product where primary antibody binds (dark brown); sections were counterstained with Harry's hematoxylin. Arrows indicate the positive staining for DCs in control animals; these cells appeared small and localized in the stratum basale of the epidermis. Arrowheads indicate the positive staining of DCs in treated animals; these cells appeared larger than positive cells in control animals, showed dendrites, and were localized along the stratum spinosum. (b) Double-IFA detection of CD207/Langerin (red) and CD1 (green) in the epidermis of the heel-bulb. Nuclei were stained with DAPI (blue). (c) Quantification of the number of positive cells in the tissue sections was performed as described in Materials and Methods. The number of positive cells is significantly higher in IFN-treated animals than in the control animals (*, P

    Article Snippet: The bound primary antibodies were detected by the avidin-biotin-peroxidase complex technique (Vectastain ABC Kit Elite; Vector, Burlingame, CA) according to the manufacturer's instructions and developed either with 3,3′-diaminobenzidine (Dako, Glostrup, Denmark) or Fast Red TR/naphthol (Sigma, St. Louis, MO).

    Techniques: Immunohistochemistry, Avidin-Biotin Assay, Staining, Immunofluorescence

    IHC staining of iNOS- and Mx1-positive cells in the tonsils and skin of Ad5-IFN-treated animals. Tissues from the tonsils and skin of control swine (panels a to d), swine treated with Ad5-CI-pIFN-α (panels e to h), Ad5-CI-pIFN-γ (panels i to l), or Ad5-CI-pIFN-α/Ad5-CI-pIFN-γ (panels m to p) 1 day after the treatment (0 dpc) were harvested and stained to detect iNOS- or Mx1-positive cells. The bound primary antibody was detected by the avidin-biotin-peroxidase complex technique and developed with Fast Red TR/naphthol, and positivity is indicated in bright purple. Sections were counterstained with Harry's hematoxylin (blue).

    Journal: Journal of Virology

    Article Title: Interferon-Induced Protection against Foot-and-Mouth Disease Virus Infection Correlates with Enhanced Tissue-Specific Innate Immune Cell Infiltration and Interferon-Stimulated Gene Expression ▿Interferon-Induced Protection against Foot-and-Mouth Disease Virus Infection Correlates with Enhanced Tissue-Specific Innate Immune Cell Infiltration and Interferon-Stimulated Gene Expression ▿ †

    doi: 10.1128/JVI.01874-09

    Figure Lengend Snippet: IHC staining of iNOS- and Mx1-positive cells in the tonsils and skin of Ad5-IFN-treated animals. Tissues from the tonsils and skin of control swine (panels a to d), swine treated with Ad5-CI-pIFN-α (panels e to h), Ad5-CI-pIFN-γ (panels i to l), or Ad5-CI-pIFN-α/Ad5-CI-pIFN-γ (panels m to p) 1 day after the treatment (0 dpc) were harvested and stained to detect iNOS- or Mx1-positive cells. The bound primary antibody was detected by the avidin-biotin-peroxidase complex technique and developed with Fast Red TR/naphthol, and positivity is indicated in bright purple. Sections were counterstained with Harry's hematoxylin (blue).

    Article Snippet: The bound primary antibodies were detected by the avidin-biotin-peroxidase complex technique (Vectastain ABC Kit Elite; Vector, Burlingame, CA) according to the manufacturer's instructions and developed either with 3,3′-diaminobenzidine (Dako, Glostrup, Denmark) or Fast Red TR/naphthol (Sigma, St. Louis, MO).

    Techniques: Immunohistochemistry, Staining, Avidin-Biotin Assay

    Gastric mucosal defect, dog. a Absence of lining epithelium, presence of bleeding ulcer, and mononuclear inflammatory cell infiltration in periglandular connective tissue in the control group. b Regenerated lining epithelium, hypervascularization of underlying tissue, and presence of mononuclear inflammatory cell infiltration in periglandular connective tissue (H E × 200). c Poorly observed bluish stained connective tissue in mucosa of control ulcer. d Fine fibers of connective tissue below regenerated epithelium in the gastric ulcer with AM graft (Masson’s trichrome × 200). e Negative staining of collagen IA1 in the exposed granulation tissue in the control ulcer and f negative staining of subepithelial connective tissue in the gastric ulcer with AM graft (avidin–biotin–peroxidase complex method, hematoxylin counterstain × 400)

    Journal: Stem Cell Research & Therapy

    Article Title: Novel approach to gastric mucosal defect repair using fresh amniotic membrane allograft in dogs (experimental study)

    doi: 10.1186/s13287-017-0682-3

    Figure Lengend Snippet: Gastric mucosal defect, dog. a Absence of lining epithelium, presence of bleeding ulcer, and mononuclear inflammatory cell infiltration in periglandular connective tissue in the control group. b Regenerated lining epithelium, hypervascularization of underlying tissue, and presence of mononuclear inflammatory cell infiltration in periglandular connective tissue (H E × 200). c Poorly observed bluish stained connective tissue in mucosa of control ulcer. d Fine fibers of connective tissue below regenerated epithelium in the gastric ulcer with AM graft (Masson’s trichrome × 200). e Negative staining of collagen IA1 in the exposed granulation tissue in the control ulcer and f negative staining of subepithelial connective tissue in the gastric ulcer with AM graft (avidin–biotin–peroxidase complex method, hematoxylin counterstain × 400)

    Article Snippet: After deparaffinization and rehydration of tissue sections, immunohistochemical (IHC) staining was carried out using a primary antibody against collagen IA1 (Novus Biologicals, Europe) prepared in rabbits and the avidin–biotin–peroxidase complex method (Dako, LSAB + system-HRP; North America, Inc.) [ ].

    Techniques: Staining, Negative Staining, Avidin-Biotin Assay