Journal: Asian Pacific Journal of Tropical Biomedicine

Article Title: Metastatic apocrine sweat gland adenocarcinoma in a terrier dog

doi: 10.1016/S2221-1691(12)60118-X

Figure Lengend Snippet: Neoplastic cells are characterized by intense pancytokeratin expression. ABC. Bar=25 µm.

Article Snippet: Immunohistochemical staining of additional sections was performed with avidin–biotin complex (ABC) method using antibodies against pancytokeratin (1/500), cytokeratin 7 (1/500), cytokeratin 20 (1/500), and S100 protein (1/400; all from Dakocytomation, Denmark).

Techniques: Expressing

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Direct selection of a human antibody fragment directed against the tumor T-cell epitope HLA-A1-MAGE-A1 from a nonimmunized phage-Fab library

doi:

Figure Lengend Snippet: ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, biotinylated complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.

Article Snippet: The biotinylated complex was separated from the free biotin by gel filtration on a Superdex 200 column (Amersham Pharmacia).

Techniques: Activation Assay, Recombinant, CTL Assay, Clone Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Binding Assay, Mutagenesis

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Direct selection of a human antibody fragment directed against the tumor T-cell epitope HLA-A1-MAGE-A1 from a nonimmunized phage-Fab library

doi:

Figure Lengend Snippet: Examples of specificity of recombinant antibodies selected for binding to HLA-A1–MAGE-A1 complexes. Microwells were coated with biotinylated BSA, washed, incubated with streptavidin, washed, and incubated with biotinylated HLA-A1–MAGE-A1 (black bars) or HLA-A1–MAGE-A3 (white bars) complexes. Suspensions of phages displaying the recombinant Fab fragments were then added. After washing, bound phages were detected with a mAb recognizing the p8 protein. Positive and negative controls were mAbs TÜ155 and W6/32HK (HK), respectively.

Article Snippet: The biotinylated complex was separated from the free biotin by gel filtration on a Superdex 200 column (Amersham Pharmacia).

Techniques: Recombinant, Binding Assay, Incubation