avidin-biotin complex Search Results


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  • 99
    Vector Laboratories avidin biotin peroxidase complex
    Avidin Biotin Peroxidase Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 11339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher avidin biotin complex
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    Vector Laboratories avidin biotin complex
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    Agilent technologies avidin biotin peroxidase complex
    Avidin Biotin Peroxidase Complex, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories avidin biotin horseradish peroxidase complex
    Avidin Biotin Horseradish Peroxidase Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 2070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories avidin biotinylated peroxidase complex
    Avidin Biotinylated Peroxidase Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies avidin biotin complex
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    Vector Laboratories avidin biotin peroxidase complexes
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    GE Healthcare streptavidin biotinylated horseradish peroxidase complex
    Streptavidin Biotinylated Horseradish Peroxidase Complex, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories avidin biotin complex kit
    Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher avidin biotin peroxidase complex
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    Ventana Medical avidin biotin peroxidase complex
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    Vector Laboratories avidin biotin alkaline phosphatase complex
    Avidin Biotin Alkaline Phosphatase Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories vectastain elite avidin biotin complex kit
    Vectastain Elite Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories avidin biotin peroxidase complex system
    Avidin Biotin Peroxidase Complex System, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories vectastain avidin biotin complex kit
    Vectastain Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore streptavidin biotinylated hrp complex
    Detection of modification of HSA by CLV-tetraethylenglycol-Biotin (CLV-TEG-B). Human serum albumin (HSA) was incubated in the presence of CLV-TEG-B for 16 h at 37°C. HSA:CLV-TEG-B molar ratios are shown in figure (A) Aliquots of the haptenation reactions containing 2 µg of protein were analyzed by SDS-PAGE and CLV-TEG-B modification was detected by blot with <t>streptavidin-HRP</t> and ECL detection (B) 0.15 µg aliquots of HSA-CLV-TEG-B (left) and 0.006–0.377 pmol aliquots of BSA-B standards (right) were analyzed by SDS-PAGE followed by blot with streptavidin-HRP and ECL detection.
    Streptavidin Biotinylated Hrp Complex, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    The Human Complement Component C1r Biotinylated Antibody from R D Systems is a goat polyclonal antibody to Complement Component C1r This antibody reacts with human The Human Complement Component C1r
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    N/A
    The Human Complement Factor B Biotinylated Antibody from R D Systems is a goat polyclonal antibody to Complement Factor B This antibody reacts with human The Human Complement Factor B
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    N/A
    Biotinylated Recombinant Human HLA A 0201 NY ESO 1 SLLMWITQC complex Protein is produced by mammalian expression system The target protein is expressed with sequence Gly25 Thr305 HLA A 0201
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    The Human Complement Factor D Adipsin Biotinylated Antibody from R D Systems is a goat polyclonal antibody to Complement Factor D Adipsin This antibody reacts with human The Human Complement
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    Detection of modification of HSA by CLV-tetraethylenglycol-Biotin (CLV-TEG-B). Human serum albumin (HSA) was incubated in the presence of CLV-TEG-B for 16 h at 37°C. HSA:CLV-TEG-B molar ratios are shown in figure (A) Aliquots of the haptenation reactions containing 2 µg of protein were analyzed by SDS-PAGE and CLV-TEG-B modification was detected by blot with streptavidin-HRP and ECL detection (B) 0.15 µg aliquots of HSA-CLV-TEG-B (left) and 0.006–0.377 pmol aliquots of BSA-B standards (right) were analyzed by SDS-PAGE followed by blot with streptavidin-HRP and ECL detection.

    Journal: Frontiers in Pharmacology

    Article Title: Biotin-Labelled Clavulanic Acid to Identify Proteins Target for Haptenation in Serum: Implications in Allergy Studies

    doi: 10.3389/fphar.2020.594755

    Figure Lengend Snippet: Detection of modification of HSA by CLV-tetraethylenglycol-Biotin (CLV-TEG-B). Human serum albumin (HSA) was incubated in the presence of CLV-TEG-B for 16 h at 37°C. HSA:CLV-TEG-B molar ratios are shown in figure (A) Aliquots of the haptenation reactions containing 2 µg of protein were analyzed by SDS-PAGE and CLV-TEG-B modification was detected by blot with streptavidin-HRP and ECL detection (B) 0.15 µg aliquots of HSA-CLV-TEG-B (left) and 0.006–0.377 pmol aliquots of BSA-B standards (right) were analyzed by SDS-PAGE followed by blot with streptavidin-HRP and ECL detection.

    Article Snippet: All conjugates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by modification detection with streptavidin-horseradish peroxidase (HRP) and ECL , as described below.

    Techniques: Modification, Incubation, SDS Page

    Detection of modification of serum proteins by clavulanic acid (CLV)-tetraethylenglycol-Biotin (CLV-TEG-B) and identification of targets. Human sera were incubated in the presence of CLV-TEG-B for 16 h at 37°C. (A) 4 µg aliquots of resulting adducts were analyzed by SDS-PAGE and CLV-TEG-B modification was detected by blot with streptavidin-HRP and ECL detection. Lower panel shows the Coomassie staining for total proteins visualization. (B) 50 µg aliquots of resulting adducts were subjected to 2D-eletrophoresis on duplicate gels, after which, one of the gels was used for detection of modified proteins by transfer to membrane and biotin detection with avidin-HRP and the other one was used for protein staining with Coomassie. Matched spots were excised from the gel and used for tryptic digestion and peptide fingerprint analysis. The blot in the middle panel is deliberately overexposed in order to show the signals corresponding to modified immunoglobulin chains. The red arrow point towards a spot that could be proposed as transferrin, on the bases of its molecular weight and isoelectric point.

    Journal: Frontiers in Pharmacology

    Article Title: Biotin-Labelled Clavulanic Acid to Identify Proteins Target for Haptenation in Serum: Implications in Allergy Studies

    doi: 10.3389/fphar.2020.594755

    Figure Lengend Snippet: Detection of modification of serum proteins by clavulanic acid (CLV)-tetraethylenglycol-Biotin (CLV-TEG-B) and identification of targets. Human sera were incubated in the presence of CLV-TEG-B for 16 h at 37°C. (A) 4 µg aliquots of resulting adducts were analyzed by SDS-PAGE and CLV-TEG-B modification was detected by blot with streptavidin-HRP and ECL detection. Lower panel shows the Coomassie staining for total proteins visualization. (B) 50 µg aliquots of resulting adducts were subjected to 2D-eletrophoresis on duplicate gels, after which, one of the gels was used for detection of modified proteins by transfer to membrane and biotin detection with avidin-HRP and the other one was used for protein staining with Coomassie. Matched spots were excised from the gel and used for tryptic digestion and peptide fingerprint analysis. The blot in the middle panel is deliberately overexposed in order to show the signals corresponding to modified immunoglobulin chains. The red arrow point towards a spot that could be proposed as transferrin, on the bases of its molecular weight and isoelectric point.

    Article Snippet: All conjugates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by modification detection with streptavidin-horseradish peroxidase (HRP) and ECL , as described below.

    Techniques: Modification, Incubation, SDS Page, Staining, Avidin-Biotin Assay, Molecular Weight

    Competition assay between clavulanic acid (CLV) and CLV-tetraethylenglycol-Biotin (CLV-TEG-B). Human serum albumin (HSA) was preincubated with increasing concentrations of CLV (80, 800 and 8,000 μM) for 16 h at 37°C and then with a fixed concentration of CLV-TEG-B (80 μM) for 2 h at 37°C. Resulting adducts were analyzed by SDS-PAGE and CLV-TEG-B modification was detected by blot with streptavidin-HRP and ECL detection (top panel) . Total protein was detected by Coomassie staining (bottom panel) .

    Journal: Frontiers in Pharmacology

    Article Title: Biotin-Labelled Clavulanic Acid to Identify Proteins Target for Haptenation in Serum: Implications in Allergy Studies

    doi: 10.3389/fphar.2020.594755

    Figure Lengend Snippet: Competition assay between clavulanic acid (CLV) and CLV-tetraethylenglycol-Biotin (CLV-TEG-B). Human serum albumin (HSA) was preincubated with increasing concentrations of CLV (80, 800 and 8,000 μM) for 16 h at 37°C and then with a fixed concentration of CLV-TEG-B (80 μM) for 2 h at 37°C. Resulting adducts were analyzed by SDS-PAGE and CLV-TEG-B modification was detected by blot with streptavidin-HRP and ECL detection (top panel) . Total protein was detected by Coomassie staining (bottom panel) .

    Article Snippet: All conjugates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by modification detection with streptavidin-horseradish peroxidase (HRP) and ECL , as described below.

    Techniques: Competitive Binding Assay, Concentration Assay, SDS Page, Modification, Staining