avidin-biotin complex Search Results


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  • 99
    Vector Laboratories avidin biotin complex
    Avidin Biotin Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 10482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore avidin biotin complex
    Avidin Biotin Complex, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies avidin biotin complex
    Neoplastic cells are characterized by intense <t>pancytokeratin</t> expression. <t>ABC.</t> Bar=25 µm.
    Avidin Biotin Complex, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 855 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioTek Instruments avidin biotin complex
    Neoplastic cells are characterized by intense <t>pancytokeratin</t> expression. <t>ABC.</t> Bar=25 µm.
    Avidin Biotin Complex, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam avidin biotin complex
    Neoplastic cells are characterized by intense <t>pancytokeratin</t> expression. <t>ABC.</t> Bar=25 µm.
    Avidin Biotin Complex, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime avidin biotin complex
    Neoplastic cells are characterized by intense <t>pancytokeratin</t> expression. <t>ABC.</t> Bar=25 µm.
    Avidin Biotin Complex, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boster Bio avidin biotin complex
    Neoplastic cells are characterized by intense <t>pancytokeratin</t> expression. <t>ABC.</t> Bar=25 µm.
    Avidin Biotin Complex, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare biotinylated complex
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Biotinylated Complex, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology avidin biotin complex
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complex, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EastCoast Bio avidin biotin complex
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complex, supplied by EastCoast Bio, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher avidin biotin complex
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems avidin biotin complex
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complex, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meda AB avidin biotin complex
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complex, supplied by Meda AB, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Agilent technologies avidin biotin complexes
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complexes, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology avidin biotin complex reagent
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complex Reagent, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher avidin biotin complex reagent
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complex Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NEN Life Science avidin biotin complex technique
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complex Technique, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies avidin biotin immunoperoxidase complex
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Immunoperoxidase Complex, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Novocastra avidin biotin complex technique
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complex Technique, supplied by Novocastra, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies avidin biotin complex reagent
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complex Reagent, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore avidin biotin complexes
    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, <t>biotinylated</t> complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.
    Avidin Biotin Complexes, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Neoplastic cells are characterized by intense pancytokeratin expression. ABC. Bar=25 µm.

    Journal: Asian Pacific Journal of Tropical Biomedicine

    Article Title: Metastatic apocrine sweat gland adenocarcinoma in a terrier dog

    doi: 10.1016/S2221-1691(12)60118-X

    Figure Lengend Snippet: Neoplastic cells are characterized by intense pancytokeratin expression. ABC. Bar=25 µm.

    Article Snippet: Immunohistochemical staining of additional sections was performed with avidin–biotin complex (ABC) method using antibodies against pancytokeratin (1/500), cytokeratin 7 (1/500), cytokeratin 20 (1/500), and S100 protein (1/400; all from Dakocytomation, Denmark).

    Techniques: Expressing

    ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, biotinylated complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Direct selection of a human antibody fragment directed against the tumor T-cell epitope HLA-A1-MAGE-A1 from a nonimmunized phage-Fab library

    doi:

    Figure Lengend Snippet: ( A ) T cell activation assay with the recombinant HLA-A1–MAGE-A1 complexes. Microwells were coated with the indicated concentrations of HLA-A1–MAGE-A1 (A1MA1) or HLA-A2–MAGE-A3 (A2MA3) complexes, and washed. CTL clones 82/30 (anti-HLA-A1–MAGE-A1) or 413/13 (against a peptide presented on HLA-A2 molecules) were added at 3,000 cells per well. After 24 h, the concentration of TNF present in the culture medium was measured by testing its cytotoxicity on the TNF-sensitive WEHI-164c13 cells. ( B ) ELISA with anti-HLA mAbs. Equivalent amounts of proteins were coated directly on plastic (striped, aggregates; gray, β2m; and white, complex) or on streptavidin-coated plastic (black, biotinylated complex) and binding of several mAbs was tested. PBS, no antibody; W6/32, mAb W6/32HL binding to heavy chain–β2m dimers; HK, inactive mutant of W6/32HK; HB28, mAb binding to β2m; HC-A2, mAb binding to a nonconformational epitope of HLA molecules; and TÜ114 and TÜ155, two conformation-sensitive mAbs.

    Article Snippet: The biotinylated complex was separated from the free biotin by gel filtration on a Superdex 200 column (Amersham Pharmacia).

    Techniques: Activation Assay, Recombinant, CTL Assay, Clone Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Binding Assay, Mutagenesis

    Examples of specificity of recombinant antibodies selected for binding to HLA-A1–MAGE-A1 complexes. Microwells were coated with biotinylated BSA, washed, incubated with streptavidin, washed, and incubated with biotinylated HLA-A1–MAGE-A1 (black bars) or HLA-A1–MAGE-A3 (white bars) complexes. Suspensions of phages displaying the recombinant Fab fragments were then added. After washing, bound phages were detected with a mAb recognizing the p8 protein. Positive and negative controls were mAbs TÜ155 and W6/32HK (HK), respectively.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Direct selection of a human antibody fragment directed against the tumor T-cell epitope HLA-A1-MAGE-A1 from a nonimmunized phage-Fab library

    doi:

    Figure Lengend Snippet: Examples of specificity of recombinant antibodies selected for binding to HLA-A1–MAGE-A1 complexes. Microwells were coated with biotinylated BSA, washed, incubated with streptavidin, washed, and incubated with biotinylated HLA-A1–MAGE-A1 (black bars) or HLA-A1–MAGE-A3 (white bars) complexes. Suspensions of phages displaying the recombinant Fab fragments were then added. After washing, bound phages were detected with a mAb recognizing the p8 protein. Positive and negative controls were mAbs TÜ155 and W6/32HK (HK), respectively.

    Article Snippet: The biotinylated complex was separated from the free biotin by gel filtration on a Superdex 200 column (Amersham Pharmacia).

    Techniques: Recombinant, Binding Assay, Incubation