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  • 99
    Thermo Fisher surveyor autosampler plus autosampler
    Summary of robotic workflow. (a) Starting position: DBS is mounted on the microtitre plate. One well contains extraction solvent and a second well contains trypsin solution; (b) 7 μL of 50 mMol NH 4 HCO 3 is aspirated from solvent well; (c) 6 μL is dispensed onto DBS surface. Liquid microjunction is maintained between the pipette tip and the DBS surface (for 4 s) allowing intact proteins to dissolve into solvent; (d) solution of intact proteins (5 μL) is re-aspirated and dispensed into clean sample well; (e) 4.5 μL of 0.1 μg/μL trypsin solution is aspirated from trypsin well; (f) trypsin solution is added to sample well; (g) sample is incubated at 40 °C for 1 h. Enzyme digests intact proteins into peptides; (h) and (i) as solvent begins to evaporate from sample well, additional solvent (7.5 μL) is aspirated from solvent well and added to sample well [ (h) and (i) are performed at 30 min and 1 h]. (j) Proteins are digested into peptides after 1 h. (k) Plate is transferred to HPLC <t>autosampler</t> and peptides are analyzed by LC MS/MS
    Surveyor Autosampler Plus Autosampler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/surveyor autosampler plus autosampler/product/Thermo Fisher
    Average 99 stars, based on 365 article reviews
    Price from $9.99 to $1999.99
    surveyor autosampler plus autosampler - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Bruker Corporation autosampler
    Summary of robotic workflow. (a) Starting position: DBS is mounted on the microtitre plate. One well contains extraction solvent and a second well contains trypsin solution; (b) 7 μL of 50 mMol NH 4 HCO 3 is aspirated from solvent well; (c) 6 μL is dispensed onto DBS surface. Liquid microjunction is maintained between the pipette tip and the DBS surface (for 4 s) allowing intact proteins to dissolve into solvent; (d) solution of intact proteins (5 μL) is re-aspirated and dispensed into clean sample well; (e) 4.5 μL of 0.1 μg/μL trypsin solution is aspirated from trypsin well; (f) trypsin solution is added to sample well; (g) sample is incubated at 40 °C for 1 h. Enzyme digests intact proteins into peptides; (h) and (i) as solvent begins to evaporate from sample well, additional solvent (7.5 μL) is aspirated from solvent well and added to sample well [ (h) and (i) are performed at 30 min and 1 h]. (j) Proteins are digested into peptides after 1 h. (k) Plate is transferred to HPLC <t>autosampler</t> and peptides are analyzed by LC MS/MS
    Autosampler, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/autosampler/product/Bruker Corporation
    Average 99 stars, based on 240 article reviews
    Price from $9.99 to $1999.99
    autosampler - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Summary of robotic workflow. (a) Starting position: DBS is mounted on the microtitre plate. One well contains extraction solvent and a second well contains trypsin solution; (b) 7 μL of 50 mMol NH 4 HCO 3 is aspirated from solvent well; (c) 6 μL is dispensed onto DBS surface. Liquid microjunction is maintained between the pipette tip and the DBS surface (for 4 s) allowing intact proteins to dissolve into solvent; (d) solution of intact proteins (5 μL) is re-aspirated and dispensed into clean sample well; (e) 4.5 μL of 0.1 μg/μL trypsin solution is aspirated from trypsin well; (f) trypsin solution is added to sample well; (g) sample is incubated at 40 °C for 1 h. Enzyme digests intact proteins into peptides; (h) and (i) as solvent begins to evaporate from sample well, additional solvent (7.5 μL) is aspirated from solvent well and added to sample well [ (h) and (i) are performed at 30 min and 1 h]. (j) Proteins are digested into peptides after 1 h. (k) Plate is transferred to HPLC autosampler and peptides are analyzed by LC MS/MS

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Dried Blood Spot Proteomics: Surface Extraction of Endogenous Proteins Coupled with Automated Sample Preparation and Mass Spectrometry Analysis

    doi: 10.1007/s13361-013-0658-1

    Figure Lengend Snippet: Summary of robotic workflow. (a) Starting position: DBS is mounted on the microtitre plate. One well contains extraction solvent and a second well contains trypsin solution; (b) 7 μL of 50 mMol NH 4 HCO 3 is aspirated from solvent well; (c) 6 μL is dispensed onto DBS surface. Liquid microjunction is maintained between the pipette tip and the DBS surface (for 4 s) allowing intact proteins to dissolve into solvent; (d) solution of intact proteins (5 μL) is re-aspirated and dispensed into clean sample well; (e) 4.5 μL of 0.1 μg/μL trypsin solution is aspirated from trypsin well; (f) trypsin solution is added to sample well; (g) sample is incubated at 40 °C for 1 h. Enzyme digests intact proteins into peptides; (h) and (i) as solvent begins to evaporate from sample well, additional solvent (7.5 μL) is aspirated from solvent well and added to sample well [ (h) and (i) are performed at 30 min and 1 h]. (j) Proteins are digested into peptides after 1 h. (k) Plate is transferred to HPLC autosampler and peptides are analyzed by LC MS/MS

    Article Snippet: The microtitre plate was removed from the Triversa Nanomate and placed in the autosampler of the HPLC system (Ultimate 3000; Dionex, Thermo Fisher Scientific, Loughborough, UK), which is coupled to a Thermo Fisher Orbitrap Velos ETD mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) via the Triversa Nanomate.

    Techniques: Transferring, Incubation, High Performance Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Schematic for two-column labeling setup. Valve 1 is housed on the autosampler, Valve 2 is housed in the LC column oven, and Valve 3 is an external valve housed on the MS stage. Valve 2 is a 10-port valve but is depicted with 6-ports for clarity. White

    Journal: Analytical chemistry

    Article Title: On-column Dimethylation with Capillary Liquid Chromatography-Tandem Mass Spectrometry for Online Determination of Neuropeptides in Rat Brain Microdialysate

    doi: 10.1021/acs.analchem.7b04965

    Figure Lengend Snippet: Schematic for two-column labeling setup. Valve 1 is housed on the autosampler, Valve 2 is housed in the LC column oven, and Valve 3 is an external valve housed on the MS stage. Valve 2 is a 10-port valve but is depicted with 6-ports for clarity. White

    Article Snippet: A volume of 350 μL of the TEAA buffer was placed into an autosampler vial (Thermo Scientific, Rockwood, TN) followed by 3.50 μL of 0.6 M sodium cyanoborohydride (Sigma) and 3.50 μL of 4% formaldehyde (J.T.

    Techniques: Labeling, Mass Spectrometry

    Schematic design workflow for the isobaric tandem mass tag (TMT 6 ) quantification. HEK293 protein lysate was labeled in a sixplex TMT format: 126 and 127 for online MudPit, 128 and 129 for the offline MudPit, and 130 and 131 for the offline with autosampler MudPit (offline-AS). Directly after labeling, an equal volume of the TMT tags was mixed and analyzed to ensure successful labeling (labeling efficiency averaged 97.2%). Samples were then processed with different MudPit platforms, massed up to same volume with 100 mM TEAB, and equally combined into one sample before drying and mass analysis.

    Journal: Journal of Proteome Research

    Article Title: Off-Line Multidimensional Liquid Chromatography and Auto Sampling Result in Sample Loss in LC/LC–MS/MS

    doi: 10.1021/pr500530e

    Figure Lengend Snippet: Schematic design workflow for the isobaric tandem mass tag (TMT 6 ) quantification. HEK293 protein lysate was labeled in a sixplex TMT format: 126 and 127 for online MudPit, 128 and 129 for the offline MudPit, and 130 and 131 for the offline with autosampler MudPit (offline-AS). Directly after labeling, an equal volume of the TMT tags was mixed and analyzed to ensure successful labeling (labeling efficiency averaged 97.2%). Samples were then processed with different MudPit platforms, massed up to same volume with 100 mM TEAB, and equally combined into one sample before drying and mass analysis.

    Article Snippet: Fractions were then loaded into a 2.5 RP resin column (offline MudPIT) or purified by stage tip and placed in autosampler vials (offline MudPIT with autosampler [EASY-nLC II, Thermo]).

    Techniques: Labeling

    Tandem mass tag (TMT) isobaric quantification of different MudPit panels. Reporter ion intensities for highly abundant (A) and low abundant (B) peptides were plotted with trend line pattern (dashed red line). (C) Perpendicular 3D plot revealed that most of the identified reporter ions were relatively higher in online MudPit with respect to the other offline formats. (D) Frequency histogram of MudPit panels showing distribution of log 2 peptide ratio observed between compared groups. (E) Log–Log correlation plot of protein expression ratio of the online panel over offline panels. Black and red dots represents proteins with higher intensities in online module with 1.4 to 2 fold, respectively. (F) Example spectrum for peptide labeled with TMT isobaric mass tag labeling reagent. The MS/MS fragmentations were used to sequence the peptide. On the basis of the amino acid ladder, the peptide was identified as VNPTVFFDIAVDGEPLGR with the N-terminus modified by TMT isobaric mass tag labeling reagent. This peptide belongs to peptidylprolyl isomerase (PPIA). Mass tags (126–131) observed in the lower m / z region (inserted figure) indicate the relative abundance of this peptide in each group. The samples were labeled in the following order: online MudPIT (126, 127), offline MudPIT (128, 129), and offline MudPIT with autosampler (130, 131).

    Journal: Journal of Proteome Research

    Article Title: Off-Line Multidimensional Liquid Chromatography and Auto Sampling Result in Sample Loss in LC/LC–MS/MS

    doi: 10.1021/pr500530e

    Figure Lengend Snippet: Tandem mass tag (TMT) isobaric quantification of different MudPit panels. Reporter ion intensities for highly abundant (A) and low abundant (B) peptides were plotted with trend line pattern (dashed red line). (C) Perpendicular 3D plot revealed that most of the identified reporter ions were relatively higher in online MudPit with respect to the other offline formats. (D) Frequency histogram of MudPit panels showing distribution of log 2 peptide ratio observed between compared groups. (E) Log–Log correlation plot of protein expression ratio of the online panel over offline panels. Black and red dots represents proteins with higher intensities in online module with 1.4 to 2 fold, respectively. (F) Example spectrum for peptide labeled with TMT isobaric mass tag labeling reagent. The MS/MS fragmentations were used to sequence the peptide. On the basis of the amino acid ladder, the peptide was identified as VNPTVFFDIAVDGEPLGR with the N-terminus modified by TMT isobaric mass tag labeling reagent. This peptide belongs to peptidylprolyl isomerase (PPIA). Mass tags (126–131) observed in the lower m / z region (inserted figure) indicate the relative abundance of this peptide in each group. The samples were labeled in the following order: online MudPIT (126, 127), offline MudPIT (128, 129), and offline MudPIT with autosampler (130, 131).

    Article Snippet: Fractions were then loaded into a 2.5 RP resin column (offline MudPIT) or purified by stage tip and placed in autosampler vials (offline MudPIT with autosampler [EASY-nLC II, Thermo]).

    Techniques: Expressing, Labeling, Mass Spectrometry, Sequencing, Modification

    Schematic design workflow for the label-free MudPIT platforms quantification. (A) HEK293 protein lysate was digested and processed in three replicate runs with different MudPIT panels: online (automated panel), offline (manual collection of fractions), and offline-AS (offline with autosampler where fractions were collected manually then cleaned up with C18 stage tip columns before being placed into autosampler). (B) Offline fractions were collected for 5 min after the volatile salt pulse phase using 10–100% ammonium acetate.

    Journal: Journal of Proteome Research

    Article Title: Off-Line Multidimensional Liquid Chromatography and Auto Sampling Result in Sample Loss in LC/LC–MS/MS

    doi: 10.1021/pr500530e

    Figure Lengend Snippet: Schematic design workflow for the label-free MudPIT platforms quantification. (A) HEK293 protein lysate was digested and processed in three replicate runs with different MudPIT panels: online (automated panel), offline (manual collection of fractions), and offline-AS (offline with autosampler where fractions were collected manually then cleaned up with C18 stage tip columns before being placed into autosampler). (B) Offline fractions were collected for 5 min after the volatile salt pulse phase using 10–100% ammonium acetate.

    Article Snippet: Fractions were then loaded into a 2.5 RP resin column (offline MudPIT) or purified by stage tip and placed in autosampler vials (offline MudPIT with autosampler [EASY-nLC II, Thermo]).

    Techniques: