Journal: Journal of Nanomaterials

Article Title: Ultrasensitive Detection of Lysozyme upon Conformational Change of DNA Duplex

doi: 10.1155/2022/1107081

Figure Lengend Snippet: Figure 1: Characterization of AuNPs. (a) The UV–Vis absorbance spectrum of AuNPs; (b) size distribution of AuNPs measured by Zetasizer Nano ZS (Malvern Instruments); (c) morphology and size of AuNPs with diameter of ~14 nm (TEM).

Article Snippet: Wavelength (nm) (a) 1 6 11 16 21 26 1 10 100 In te ns ity (p er ce nt ) Size (d. nm) (b) (c) Figure 1: Characterization of AuNPs. (a) The UV–Vis absorbance spectrum of AuNPs; (b) size distribution of AuNPs measured by Zetasizer Nano ZS (Malvern Instruments); (c) morphology and size of AuNPs with diameter of ~14 nm (TEM). nloaded from https://onlinelibrary.w iley.com /doi/10.1155/2022/1107081 by IN A SP - N E PA L , W iley O nline L ibrary on [30/12/2024].

Techniques:

Journal: Nanoscale Research Letters

Article Title: T cells enhance gold nanoparticle delivery to tumors in vivo

doi: 10.1186/1556-276X-6-283

Figure Lengend Snippet: Biodistribution comparison of AuNPs and AuNP-T cells in mice . Mice were injected with PEG-AuNPs (60-65 nm hydrodynamic diameter), AuNP-T cells, or PBS and subsequently sacrificed at various time points to determine biodistribution. PBS gold levels were negligible in comparison to AuNP and AuNP-T cell groups for all organs. Values are percentage of the injected gold dose (%ID) were calculated from ICP-MS and are normalized for dry weight differences. The AuNP-T cell group exhibited significantly higher gold delivery to the lungs, liver, and bone, while the AuNP group demonstrated higher gold levels within the small intestine. No significant differences were seen in the spleen, kidney, muscle, or brain. An asterisk indicates statistically significant ( P < 0.05) differences.

Article Snippet: We next performed a comprehensive in vivo biodistribution study using inductively coupled plasma mass spectrometry (ICP-MS) and ICP-OES to map the location of free PEGylated AuNPs (40-45 nm gold colloid coated with 5000 MW PEG) and AuNPs delivered by T cells.

Techniques: Comparison, Injection

Journal: Nanoscale Research Letters

Article Title: T cells enhance gold nanoparticle delivery to tumors in vivo

doi: 10.1186/1556-276X-6-283

Figure Lengend Snippet: AuNP-T cells enhance the delivery of gold nanoparticles to the tumor site in vivo . Tumor-bearing mice were injected i.v. with PEG-AuNPs or AuNP-T cells. Tumors were subsequently resected at various time points and measured for AuNP content using ICP-MS. Values displayed represent the percentage of injected gold normalized for tumor dry weight differences (mean ± SEM). The percentage of gold delivered by the AuNP-T cells at 48 h represents a significant, four-fold increase over the PEG-AuNP group at 24 h ( P < 0.01).

Article Snippet: We next performed a comprehensive in vivo biodistribution study using inductively coupled plasma mass spectrometry (ICP-MS) and ICP-OES to map the location of free PEGylated AuNPs (40-45 nm gold colloid coated with 5000 MW PEG) and AuNPs delivered by T cells.

Techniques: In Vivo, Injection

Journal: Journal of Nanobiotechnology

Article Title: The effect of phospho-peptide on the stability of gold nanoparticles and drug delivery

doi: 10.1186/s12951-019-0522-y

Figure Lengend Snippet: Systematic investigation of different amino acids within scaffold peptides in stabilizing AuNPs. a Typical TEM image of AuNPs in water solution (scale bar = 100 nm). b UV–Vis absorbance of AuNPs at 400–800 nm with a typical plasmon peak at ~ 520 nm. c Histogram depicting the size of 450 particles with mean diameter at 11 nm. d Digital image of AuNPs modified by various peptides, blue color indicates aggregated AuNPs and bright red color indicates stabilized AuNPs. e The ratio of absorbance at 615 nm to that at 520 nm to show the stability of peptide modified AuNPs

Article Snippet: In this work, pY stabilized AuNPs were used as a nanocarrier for drug delivery and it was highly efficient in killing cancer cells.

Techniques: Modification

Journal: Journal of Nanobiotechnology

Article Title: The effect of phospho-peptide on the stability of gold nanoparticles and drug delivery

doi: 10.1186/s12951-019-0522-y

Figure Lengend Snippet: UV–Vis spectra of pY stabilized AuNPs in different conditions. a UV–Vis spectra of AuNPs modified by phosphorylated and non-phosphorylated peptides. b UV–Vis spectra of AuNPs modified by different length peptides (peptide sequences listed in Additional file : Table S2). c UV–Vis spectra of C-pY-Ahx modified AuNPs with various pH from 3 to 12. d UV–Vis spectra of C-pY-Ahx modified AuNPs incubated in different solutions

Article Snippet: In this work, pY stabilized AuNPs were used as a nanocarrier for drug delivery and it was highly efficient in killing cancer cells.

Techniques: Modification, Incubation

Journal: Journal of Nanobiotechnology

Article Title: The effect of phospho-peptide on the stability of gold nanoparticles and drug delivery

doi: 10.1186/s12951-019-0522-y

Figure Lengend Snippet: Schematic illustration of pY-AuNPs as a targeted drug delivery system. pY stabilized AuNPs were used as a nano-carrier for drugs (i.e., SH-Hyz-Dox). AuNPs aggregation occurs following dephosphorylation, thus increasing drug tumor retention

Article Snippet: In this work, pY stabilized AuNPs were used as a nanocarrier for drug delivery and it was highly efficient in killing cancer cells.

Techniques: De-Phosphorylation Assay

Journal: International Journal of Nanomedicine

Article Title: Gold nanoparticles enhance TRAIL sensitivity through Drp1-mediated apoptotic and autophagic mitochondrial fission in NSCLC cells

doi: 10.2147/IJN.S129274

Figure Lengend Snippet: AuNPs sensitize NSCLC cells to TRAIL. Notes: ( A ) Calu-1 cells, A549 cells, and H460 cells were exposed to the indicated concentrations of TRAIL for 24 h, and cell viability was then analyzed by MTT assay. ( B ) The size effect of AuNPs on BEAS-2B cells. BEAS-2B cells were exposed to AuNPs at the doses up to 100 μg/mL for 24 h, and then cell viability was measured using the MTT assay. * P <0.05, compared to untreated control. ( C ) The size effect of AuNPs on TRAIL sensitivity. Calu-1 cells were pretreated with the indicated concentrations of various AuNPs for 6 h, followed by incubation with 20 ng/mL TRAIL for an additional 24 h, and then cell viability was determined by MTT assay. Values are mean ± SD (n=3). * P <0.05, ** P <0.01 compared to groups without TRAIL treatment. ( D ) Cellular uptake of various AuNPs was quantified using light SSC properties by flow cytometry. The graph shows the uptake amount of nanoparticles after incubation with 50 μg/mL AuNPs at different time points. ( E ) Typical flow cytometric SSC data at 6 h post-treatment were presented. Changes in cellular SSC following treatment reflected the uptake potential of the particles. Abbreviations: AuNPs, gold nanoparticles; MTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NSCLC, non-small-cell lung cancer; SD, standard deviation; SSC, side scatter; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.

Article Snippet: Samples were then trypsinized and suspended in medium, and the uptake amount of AuNPs was analyzed with FACScan flow cytometry (BD Biosciences).

Techniques: MTT Assay, Incubation, Flow Cytometry, Standard Deviation

Journal: International Journal of Nanomedicine

Article Title: Gold nanoparticles enhance TRAIL sensitivity through Drp1-mediated apoptotic and autophagic mitochondrial fission in NSCLC cells

doi: 10.2147/IJN.S129274

Figure Lengend Snippet: TRAIL combined with AuNPs triggers autophagy. Notes: ( A , B ) Calu-1 cells were exposed to TRAIL and AuNPs, alone and together, for 24 h, and then cell lysates were processed for immunoblotting analysis using antibodies against LC3 and p62. β-actin served as a loading control. Densitometric analysis was carried out to evaluate the relative levels of LC3-II and p62. ( C , D ) Formation of GFP-LC3 puncta induced by TRAIL combined with AuNPs. Cells were transiently transfected with the plasmid expressing GFP-LC3. At 48 h after transfection, cells were exposed to TRAIL and/or AuNPs in the absence or presence of 2 mM 3-MA, and GFP-LC3-labeled autophagic puncta formation was observed with a fluorescence microscope. Statistical analysis of the number of GFP-LC3 puncta per cell 24 h after treatment. Magnification ×200. ( E , F ) TRAIL combined with AuNPs induced mitophagy. Upon treatment with TRAIL and AuNPs, alone and together, the cytosolic and mitochondrial expression of PINK1 and Parkin was examined by immunoblotting analysis. Densitometric analysis was performed to estimate the relative intensity of PINK1 and Parkin. β-actin and COX IV were used as mito and cytoplasmic markers, respectively. * P <0.05, compared to TRAIL-treated groups. ( G ) Inhibition of autophagy decreased apoptosis induced by co-treatment with TRAIL and AuNPs. Cells were exposed to combination treatment with or without 3-MA. Total cell lysates were subjected to immunoblotting analysis. ( H ) Apoptosis was evaluated by flow cytometry using the sub-G1 assay. * P <0.05, compared to the TRAIL and AuNPs group. Abbreviations: 3-MA, 3-methyladenine; AuNPs, gold nanoparticles; cyto, cytoplasm; mito, mitochondria; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.

Article Snippet: Samples were then trypsinized and suspended in medium, and the uptake amount of AuNPs was analyzed with FACScan flow cytometry (BD Biosciences).

Techniques: Western Blot, Transfection, Plasmid Preparation, Expressing, Labeling, Fluorescence, Microscopy, Inhibition, Flow Cytometry