Journal: Kidney Research and Clinical Practice

Article Title: Active maintenance of endothelial cells prevents kidney fibrosis

doi: 10.23876/j.krcp.2017.36.4.329

Figure Lengend Snippet: (A, B) Significantly reduced interstitial fibrosis following treatment with 12-(3-adamantan-1-ylureido)-dodecanoic acid (AUDA) in a unilateral ureteral obstruction (UUO) model. (C–E) Immunofluorescence analysis. AUDA significantly reduced the level of fibroblast-specific protein 1 (FSP-1) positive fibrosis, the proportion of FSP-1/CD31-positive cells, and preserved the peritubular capillary network. Arrows indicate FSP-1/CD3 double-positive cells. (F–H) AUDA also reduced the level of αSMA-positive fibrosis and the proportion of αSMA/CD31 double-positive cells. (I, J) Inhibition of sEH down-regulated expression of FSP-1 as demonstrated by real-time PCR (I) and Western blotting (J). Values are given as mean ± standard error of mean (n = 6 per group for each experiment). * P < 0.05, ** P < 0.01, *** P < 0.001; by ANOVA test.

Article Snippet: The sEH inhibitor AUDA (8 mg/kg/day) was continuously administered via an Alzet ® implantable subcutaneous mini-osmotic pump (DURECT Co., Cupertino, CA, USA) for 1 or 2 weeks.

Techniques: Immunofluorescence, Inhibition, Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: iScience

Article Title: Soluble epoxide hydrolase maintains steady-state lipid turnover linked with autocrine signaling in peritoneal macrophages

doi: 10.1016/j.isci.2023.107465

Figure Lengend Snippet: N-phos inhibition and Ca 2+ store reduction (A) Effects of sEH inhibitors on basic Ca 2+ handing features. The Fura-2 imaging traces show averaged time courses with shaded areas indicating standard errors (n = 10 TGPMs in each group). The Ca 2+ ionophore ionomycin (IM) was used to trigger Ca 2+ release and deplete intracellular stores. The inhibitors used were the N-phos-specific inhibitor AHBA, the C-EH-specific inhibitor AUDA, and the dual inhibitor ebselen. In the dot plots, resting Fura-2 ratio, ionomycin-induced response, and SOCE amplitude are statistically analyzed. The data represent means ± SEM., and the numbers of cells and mice examined are shown in parentheses. Significant differences from the vehicle-treated group are marked with asterisks (∗∗p < 0.01 in one-way ANOVA and Dunnett’s test). (B) AHBA reduces Ca 2+ release in response to P2Y receptor activation. The imaging traces show averaged time courses with shaded areas indicating standard errors (n = 10 TGPMs in each group). In the dot plot, ATP-induced responses are statistically analyzed. A significant difference is marked with an asterisk (∗p < 0.05 in t -test).

Article Snippet: AUDA (C-EH inhibitor) , Cayman Chemical , 10007927; CAS: 479413-70-2.

Techniques: Inhibition, Imaging, Activation Assay

Journal: iScience

Article Title: Soluble epoxide hydrolase maintains steady-state lipid turnover linked with autocrine signaling in peritoneal macrophages

doi: 10.1016/j.isci.2023.107465

Figure Lengend Snippet:

Article Snippet: AUDA (C-EH inhibitor) , Cayman Chemical , 10007927; CAS: 479413-70-2.

Techniques: Purification, Derivative Assay, Recombinant, Enzyme-linked Immunosorbent Assay, Chemotaxis Assay, H2O2 Assay, Microarray, Software