att20 Search Results


att 20 cells  (ATCC)
95
ATCC att 20 cells
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Att 20 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pmc12982089-44-0-11?v=ATCC
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att 20 cells - by Bioz Stars, 2026-08
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mouse corticotrope pituitary derived cell line att  (ATCC)
95
ATCC mouse corticotrope pituitary derived cell line att
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Mouse Corticotrope Pituitary Derived Cell Line Att, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pm42116249-67-30-37?v=ATCC
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mouse corticotrope pituitary derived cell line att - by Bioz Stars, 2026-08
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pituitary tumor cell line  (ATCC)
94
ATCC pituitary tumor cell line
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Pituitary Tumor Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pmc10632222-24-3-8?v=ATCC
Average 94 stars, based on 1 article reviews
pituitary tumor cell line - by Bioz Stars, 2026-08
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att-20 cell lines  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures att-20 cell lines
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Att 20 Cell Lines, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pmc08275863-37-0-14?v=European+Collection+of+Authenticated+Cell+Cultures
Average 90 stars, based on 1 article reviews
att-20 cell lines - by Bioz Stars, 2026-08
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att-20 cells  (HORIBA Ltd)
90
HORIBA Ltd att-20 cells
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Att 20 Cells, supplied by HORIBA Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pm11739016-36-24-30?v=HORIBA+Ltd
Average 90 stars, based on 1 article reviews
att-20 cells - by Bioz Stars, 2026-08
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att-20 cells  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare att-20 cells
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Att 20 Cells, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pm10409712-37-1-13?v=Johns+Hopkins+HealthCare
Average 90 stars, based on 1 article reviews
att-20 cells - by Bioz Stars, 2026-08
90/100 stars
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mouse endocrine cell line att20/d16v-wt#6 (att20)  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc mouse endocrine cell line att20/d16v-wt#6 (att20)
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Mouse Endocrine Cell Line Att20/D16v Wt#6 (Att20), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pm08626447-53-7-19?v=STEMCELL+Technologies+Inc
Average 90 stars, based on 1 article reviews
mouse endocrine cell line att20/d16v-wt#6 (att20) - by Bioz Stars, 2026-08
90/100 stars
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att-20 cells  (Makoto USA Inc)
90
Makoto USA Inc att-20 cells
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Att 20 Cells, supplied by Makoto USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pm33152456-9-17-29?v=Makoto+USA+Inc
Average 90 stars, based on 1 article reviews
att-20 cells - by Bioz Stars, 2026-08
90/100 stars
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mouse att20 corticotrope bk channels  (STREX Inc)
90
STREX Inc mouse att20 corticotrope bk channels
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Mouse Att20 Corticotrope Bk Channels, supplied by STREX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pm10517674-84-6-26?v=STREX+Inc
Average 90 stars, based on 1 article reviews
mouse att20 corticotrope bk channels - by Bioz Stars, 2026-08
90/100 stars
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att20 cells  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies att20 cells
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Att20 Cells, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pm11852092-3-3-45?v=Federation+of+European+Neuroscience+Societies
Average 90 stars, based on 1 article reviews
att20 cells - by Bioz Stars, 2026-08
90/100 stars
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mouse pituitary cell lines att20  (National Centre for Cell Science)
90
National Centre for Cell Science mouse pituitary cell lines att20
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Mouse Pituitary Cell Lines Att20, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/ppr0307933-102-3-13?v=National+Centre+for+Cell+Science
Average 90 stars, based on 1 article reviews
mouse pituitary cell lines att20 - by Bioz Stars, 2026-08
90/100 stars
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att-20 pituitary cells  (Muegge GmbH)
90
Muegge GmbH att-20 pituitary cells
Effect of the test and control SRLs on ACTH secretion <t>in</t> <t>AtT-20</t> cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.
Att 20 Pituitary Cells, supplied by Muegge GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/att20/pm11259365-248-24-4?v=Muegge+GmbH
Average 90 stars, based on 1 article reviews
att-20 pituitary cells - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.

Journal: Frontiers in Oncology

Article Title: Anti-secretory and anti-proliferative actions of next-generation dual subtype 2 and 5 somatostatin receptor ligands in neuroendocrine tumor models

doi: 10.3389/fonc.2026.1766563

Figure Lengend Snippet: Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. Effect of the test and control SRLs on ACTH secretion in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations of (A) the control SRLs octreotide, pasireotide or (B) the test peptides, i.e. 5nM, 10nM, and 50nM. After 72h, supernatants were collected, and ACTH levels were determined by means of the mouse/Rat ACTH ELISA Kit (Abcam). The experiment was repeated 3 times with similar results. Data shown are the average of two technical replicates ± standard deviation (SD). (C, D) The decrease in ACTH secretion upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.

Article Snippet: AtT-20 cells, a murine pituitary corticotroph cell line were purchased from ATCC (CCL-89).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Standard Deviation

Effect of the test and control SRLs on the proliferation of AtT-20 cells. Effect of the test and control SRLs on the proliferation of AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations (1nM, 2, 5nM, 5nM, 10nM, 50nM) of (A) the control SRLs octreotide or pasireotide, or with (B) the test peptides, for 72h. Cell viability was then carried out with WST-1 colorimetric assay. The experiment was repeated 3 times with similar results. Data shown are the average of 3 technical replicates ± SD. NT, untreated. (C, D) The decrease in cell proliferation upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.

Journal: Frontiers in Oncology

Article Title: Anti-secretory and anti-proliferative actions of next-generation dual subtype 2 and 5 somatostatin receptor ligands in neuroendocrine tumor models

doi: 10.3389/fonc.2026.1766563

Figure Lengend Snippet: Effect of the test and control SRLs on the proliferation of AtT-20 cells. Effect of the test and control SRLs on the proliferation of AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then were treated with the indicated concentrations (1nM, 2, 5nM, 5nM, 10nM, 50nM) of (A) the control SRLs octreotide or pasireotide, or with (B) the test peptides, for 72h. Cell viability was then carried out with WST-1 colorimetric assay. The experiment was repeated 3 times with similar results. Data shown are the average of 3 technical replicates ± SD. NT, untreated. (C, D) The decrease in cell proliferation upon treatment with the test peptides is here compared with that obtained with (C) octreotide or (D) pasireotide. NT, untreated. In red are the comparisons where the test peptides performed better than the reference drugs, in black are the comparisons where the reference drugs performed better than the test peptides. (A-D) Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.

Article Snippet: AtT-20 cells, a murine pituitary corticotroph cell line were purchased from ATCC (CCL-89).

Techniques: Control, Colorimetric Assay

Effect of SMTR-002 and control SRLs on forskolin-induced cAMP production in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then treated with the indicated concentrations (10nM, 50nM, 100nM) of (A) the control SRLs octreotide or pasireotide, or with (B) SMTR-002 for 72 hours. Cells were induced with 10 µM forskolin 3 hours prior to performing the assay. The levels of cAMP were then quantified with the cAMP-Glo luminescent assay. The experiment was repeated 3 times with similar results. Data shown are the average of 3 technical replicates ± SD. Data shown are the average of 3 technical replicates ± SD. Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: ***p ≤ 0.001; ****p ≤ 0.0001.

Journal: Frontiers in Oncology

Article Title: Anti-secretory and anti-proliferative actions of next-generation dual subtype 2 and 5 somatostatin receptor ligands in neuroendocrine tumor models

doi: 10.3389/fonc.2026.1766563

Figure Lengend Snippet: Effect of SMTR-002 and control SRLs on forskolin-induced cAMP production in AtT-20 cells. (A, B) AtT-20 cells were seeded at a density of 3500 cells/well and then treated with the indicated concentrations (10nM, 50nM, 100nM) of (A) the control SRLs octreotide or pasireotide, or with (B) SMTR-002 for 72 hours. Cells were induced with 10 µM forskolin 3 hours prior to performing the assay. The levels of cAMP were then quantified with the cAMP-Glo luminescent assay. The experiment was repeated 3 times with similar results. Data shown are the average of 3 technical replicates ± SD. Data shown are the average of 3 technical replicates ± SD. Statistical analyses were carried out in GraphPad Prism using one-way ANOVA: ***p ≤ 0.001; ****p ≤ 0.0001.

Article Snippet: AtT-20 cells, a murine pituitary corticotroph cell line were purchased from ATCC (CCL-89).

Techniques: Control, Luminescence Assay