Journal: Environment international

Article Title: Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury.

doi: 10.1016/j.envint.2024.108643

Figure Lengend Snippet: Fig. 3. Early-life BPS exposure regulated OXPHOS pathway in PFC. KEGG pathway enrichment bubble map of DEGs (A). GO (Cellular component) pathway enrichment bubble map of the DEGs (B). Volcano plot of the DEGs (C). Heat map of the selected DEGs related to OXPHOS pathway in PFC (D). Early-life exposure to BPS decreased the expression ATP6V1B1 (E), ATP5K (F), NDUFC1 (G), NDUFC2 (H), NDUFA3 (M), COX6B1 (O) in PFC on PND 28. Early-life exposure to BPS decreased the expression ATP6V1B1 (I), ATP5K (J), NDUFC1 (K), NDUFC2 (L), NDUFA3 (N), COX6B1 (P) in PFC on PND 56. Data are expressed as mean ± SEM, n = 4. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the control group.

Article Snippet: Primary antibody dilutions were as follows: mouse anti-MAP2 antibody (M4403, Sigma, USA; 1:1000), rabbit anti-NFL antibody (12998-1-AP, Proteintech, USA; 1:2000), rabbit anti-ATP6V1B1 antibody (14780-1-AP, Proteintech, X. Zhang et al.

Techniques: Expressing, Control

Journal: Environment international

Article Title: Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury.

doi: 10.1016/j.envint.2024.108643

Figure Lengend Snippet: Fig. 6. BPS exposure regulated the OXPHOS pathway in cultured neurons. Exposure to BPS decreased the expression ATP6V1B1 (A), ATP5K (B), NDUFC1 (C), NDUFC2 (D), NDUFA3 (E), COX6B1 (F) in cultured neurons. Data are expressed as mean ± SEM, n = 4. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the control group.

Article Snippet: Primary antibody dilutions were as follows: mouse anti-MAP2 antibody (M4403, Sigma, USA; 1:1000), rabbit anti-NFL antibody (12998-1-AP, Proteintech, USA; 1:2000), rabbit anti-ATP6V1B1 antibody (14780-1-AP, Proteintech, X. Zhang et al.

Techniques: Cell Culture, Expressing, Control

Journal: Environment international

Article Title: Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury.

doi: 10.1016/j.envint.2024.108643

Figure Lengend Snippet: Fig. 8. Myo19 overexpression attenuated BPS-induced inhibition of the OXPHOS pathway in cultured neurons. Representative images of cultured neurons transfected lentivirus (mCherry, red) on DIV 8 (A). Representative images of immunofluorescent staining of Myo19 (green) with DAPI counterstain (blue) in cultured neurons (B). BPS-induced down-regulation of Myo19 was resumed after transfection with LV-Myo19 in cultured neurons (C). BPS-induced down-regulation of ATP6V1B1 (D), ATP5K (E), NDUFC1 (F), NDUFC2 (G), NDUFA3 (H), and COX6B1 (I) was resumed after transfection with LV-Myo19 in cultured neurons. Data are expressed as mean ± SEM, n = 4. * P < 0.05, ** P < 0.01, *** P < 0.001 as compared with the control group (Cultured neurons in this group were not transfected with lentivirus). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibody dilutions were as follows: mouse anti-MAP2 antibody (M4403, Sigma, USA; 1:1000), rabbit anti-NFL antibody (12998-1-AP, Proteintech, USA; 1:2000), rabbit anti-ATP6V1B1 antibody (14780-1-AP, Proteintech, X. Zhang et al.

Techniques: Over Expression, Inhibition, Cell Culture, Transfection, Staining, Control

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Downregulated ATP6V1B1 expression acidifies the intracellular environment of cancer cells leading to resistance to antibody-dependent cellular cytotoxicity

doi: 10.1007/s00262-020-02732-3

Figure Lengend Snippet: ATP6V1B1-KO SKBR3 cells lacked the ATP6V1B1 protein according to the western blot (a) and immunohistochemistry (b) results. c An LDH assay was complete to compare the wild-type SKBR3 and ATP6V1B1-KO cells. The cytotoxicities of the wildtype SKBR3 and ATP6V1B1-KO cells were 55.6% and 19.8%, respectively, in the presence of 0.1 μg/ml trastuzumab (p < 0.01), and 81.5% and 39.1%, respectively, in the presence of 1 μg/ml trastuzumab (p < 0.01). N = 3 for all experiments. *p < 0.05, ****p < 0.0001. The graph shows mean ± SEM. d An LDH assay was complete to compare the wild-type JIMT-1 and ATP6V1B1-KO cells. The cytotoxicities of the wild-type JIMT-1 and ATP6V1B1-KO cells were 39.7% and 19.7%, respectively, in the presence of 1 μg/ml trastuzumab (p < 0.01), and 43.6% and 12.9%, respectively, in the presence of 10 μg/ml trastuzumab (p < 0.05). N = 3 for all experiments. **p < 0.01, *p < 0.05. The graph shows mean ± SEM

Article Snippet: The proteins were transferred to a PVDF membrane, blocked with Blocking One (Nacalai Tesque), and probed with an anti-ATP6V1B1 antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Western Blot, Immunohistochemistry, Lactate Dehydrogenase Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Downregulated ATP6V1B1 expression acidifies the intracellular environment of cancer cells leading to resistance to antibody-dependent cellular cytotoxicity

doi: 10.1007/s00262-020-02732-3

Figure Lengend Snippet: a, b The ATP6V1B1 genes of SKBR3 and NCI-N 87 cells were knocked down, after which the ADCC was evaluated; a The ADCC of SKBR3 and ATP6V1B1-knockdown; b The ADCC of ATP6V1B1-knockdown NCI-N87 cells. N = 3 for all experiments. ns not significant. *p < 0.05,**p < 0.01. c, d Changes to cytotoxicities after the bafilomycin A1 (specific inhibitor of V-ATPase) treatment. Cells were cultured and treated with 400 nM bafilomycin A1 for 2 h prior to the ADCC assay. C. Wild-type SKBR3 cells vs bafilomycin-treated cells. D. Wild- type BT474 cells vs bafilomycin-treated cells. N = 3 for all experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant. All graphs show mean ± SEM

Article Snippet: The proteins were transferred to a PVDF membrane, blocked with Blocking One (Nacalai Tesque), and probed with an anti-ATP6V1B1 antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Knockdown, Cell Culture, ADCC Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Downregulated ATP6V1B1 expression acidifies the intracellular environment of cancer cells leading to resistance to antibody-dependent cellular cytotoxicity

doi: 10.1007/s00262-020-02732-3

Figure Lengend Snippet: Intracellular pH; a wild-type SKBR3 and ATP6V1B1-KO cells; b wild-type SKBR3 and SKr cells; c wild-type JIMT-1 and ATP6V1B1-KO cells. The intracellular pH was determined after a treatment with BCECF-AM. Each bar represents the mean of three determinations. **p < 0.01, *p < 0.05, ***p < 0.001. The graph shows mean ± SEM

Article Snippet: The proteins were transferred to a PVDF membrane, blocked with Blocking One (Nacalai Tesque), and probed with an anti-ATP6V1B1 antibody (Atlas Antibodies, Bromma, Sweden).

Techniques:

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Downregulated ATP6V1B1 expression acidifies the intracellular environment of cancer cells leading to resistance to antibody-dependent cellular cytotoxicity

doi: 10.1007/s00262-020-02732-3

Figure Lengend Snippet: a PBMCs were co-stained to detect CD107a (marker of NK cell activity) and CD56 (NK cell marker), which reflected the granule content released by NK cells, at 15 min after the ADCC reaction was initiated. b Wild-type SKBR3 cells (left) and ATP6V1B1-KO cells (right) were stained to detect granzymes A (upper) and B (lower) at 15 min after the ADCC reaction was initiated by flow cytometry. Grey is unstained control. c Wild-type JIMT-1 cells (left) and ATP6V1B1-KO cells (right) were stained to detect granzymes A (upper) and B (lower) at 45 min after the ADCC reaction was initiated by flow cytometry d. Microscopy analysis of wild-type SKBR3 cells (upper panels) and ATP6V1B1-KO cells (lower panels) with Alexa Fluor-labeled granzyme B during the ADCC reaction. Granzyme B of wild-type SKBR3 cells and ATP6V1B1-KO cells was stained at 15, 30, and 45 min after the ADCC reaction was initiated

Article Snippet: The proteins were transferred to a PVDF membrane, blocked with Blocking One (Nacalai Tesque), and probed with an anti-ATP6V1B1 antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Staining, Marker, Activity Assay, Flow Cytometry, Control, Microscopy, Labeling

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Downregulated ATP6V1B1 expression acidifies the intracellular environment of cancer cells leading to resistance to antibody-dependent cellular cytotoxicity

doi: 10.1007/s00262-020-02732-3

Figure Lengend Snippet: The AICC activities of wild-type SKBR3 cells and ATP6V1B1-KO cells were evaluated with an LDH assay after culturing at an effector-to-target ratio of 10:1, 20:1, and 60:1 for 4 h. N = 3 for all experiments. **p < 0.01, ****p < 0.0001. The graph shows mean ± SEM. **p < 0.01, ****p < 0.0001. The graph shows mean ± SEM

Article Snippet: The proteins were transferred to a PVDF membrane, blocked with Blocking One (Nacalai Tesque), and probed with an anti-ATP6V1B1 antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Lactate Dehydrogenase Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Downregulated ATP6V1B1 expression acidifies the intracellular environment of cancer cells leading to resistance to antibody-dependent cellular cytotoxicity

doi: 10.1007/s00262-020-02732-3

Figure Lengend Snippet: a Staining intensity was classified based on the following scale: 0, no staining; 1, partial staining; 2, overall weak staining; and 3, overall strong staining. b Distribution and intensity of the staining to detect ATP6V1B1 in human breast cancer biopsy samples (pre-chemotherapy) and samples collected during an operation (post-chemotherapy) (n = 14)

Article Snippet: The proteins were transferred to a PVDF membrane, blocked with Blocking One (Nacalai Tesque), and probed with an anti-ATP6V1B1 antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Staining

Journal: Differentiation; research in biological diversity

Article Title: Dmxl1 is required for survival in the mouse to organogenesis stages of development

doi: 10.1016/j.diff.2025.100917

Figure Lengend Snippet: Western blot analysis with ATP6V1B1 antibody in the mandible of F1729C (A) and A2330T (B) mutants at E13.5. (C, D) Quantification of band intensity of ATP6V1B1 protein normalized to total protein levels from stain free gels. Statistical values shown are from one-way ANOVA.

Article Snippet: Blots were blocked with Everyblot blocking buffer (Bio-Rad) for 10 min. Primary antibodies were DMXL1 (Proteintech, 24413–1-AP, 1:1000 and Bethyl Laboratories, A304–685A) and ATP6V1B1 (Origene TA502519S, 1:1000).

Techniques: Western Blot, Staining

Journal: Cytogenetic and genome research

Article Title: Impairment of gastric acid secretion and increase of embryonic lethality in Foxq1-deficient mice.

doi: 10.1159/000125833

Figure Lengend Snippet: Fig. 1. Expression analyses of the Foxq1 gene. ( A ) RT-PCR analysis with total RNA and Foxq1 specific primers showed the presence of a 265-bp amplified product in all examined embryonic stages (E8.5– E12.5). ( B ) Northern blot with total RNA from whole embryos (E8.5– E14.5), and from stomach of E15.5–E18.5 and different postnatal days (P1–P60). Hybridization of blot with Foxq1 cDNA showed high expres- sion of Foxq1 in the stomach of E15.5 and the expression levels main- tain equivalent through neonatal stages and adulthood. Rehybridiza- tion of the blot with -actin probe revealed the integrity of RNA load- ing. ( C ) In situ hybridization of DIG-labelled Foxq1 antisense to wild-type stomach sections. ( D ) An equivalent section was immuno- stained with anti-H + ,K + -ATPase -subunit antibody. Scale bars: panels C and D, 100 m.

Article Snippet: Monoclonal anti-H + , K + -ATPase -subunit was diluted at 1: 1000 (Acris Antibodies, Hiddenhausen, Germany).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Northern Blot, Hybridization, In Situ Hybridization, Staining

Journal: Cytogenetic and genome research

Article Title: Impairment of gastric acid secretion and increase of embryonic lethality in Foxq1-deficient mice.

doi: 10.1159/000125833

Figure Lengend Snippet: Fig. 4. Histological comparison of the gastric mucosa in the fundic region of wild-type ( A, C ) and Foxq1-deficient mice ( B, D ). ( A, B ) He- matoxylin-eosin stained sections demonstrate that the overall mor- phology is not markedly different in Foxq1-deficient mice. Immuno- histochemical staining of parietal cells with anti-H+,K+-ATPase -sub- unit ( C, D ) demonstrated no marked changes in number of parietal cells per gastric gland in wild-type and Foxq1 –/– stomach. Scale bars: panels A and B, 100 m; panels C and D, 200 m.

Article Snippet: Monoclonal anti-H + , K + -ATPase -subunit was diluted at 1: 1000 (Acris Antibodies, Hiddenhausen, Germany).

Techniques: Comparison, Staining

Journal: Cytogenetic and genome research

Article Title: Impairment of gastric acid secretion and increase of embryonic lethality in Foxq1-deficient mice.

doi: 10.1159/000125833

Figure Lengend Snippet: Fig. 6. Expression analysis of genes regulating the gastric acid secre- tion. ( A ) Total RNA was isolated from stomachs of 8-week-old Foxq1 +/+ and Foxq1 –/– mice. Blot was hybridized sequentially with probes to so- matostatin ( Sst ), gastrin ( Gast ) and H + ,K + -ATPase -subunit ( Atp4a ) and human elongation factor-1 (EF1) as a loading control. ( B ) Semi- quantative RT-PCR assays were performed to determine the expression levels of gastrin receptor ( Cckbr) , histamine H2 receptor (Hrh2), ezrin (Vil2) , and telokin (Mylk) gene in stomach of wild-type and FOXQ1- deficient mice .

Article Snippet: Monoclonal anti-H + , K + -ATPase -subunit was diluted at 1: 1000 (Acris Antibodies, Hiddenhausen, Germany).

Techniques: Expressing, Isolation, Control, Reverse Transcription Polymerase Chain Reaction