Journal: Cell reports

Article Title: Crystal structures and functional analysis of the ZnF5-WWE1-WWE2 region of PARP13/ZAP define a distinctive mode of engaging poly(ADP-ribose)

doi: 10.1016/j.celrep.2022.111529

Figure Lengend Snippet:

Article Snippet: ATP-FAM labeled on gamma-phosphate , Jena-Bioscience , CatNU-833-5FM.

Techniques: Virus, Recombinant, Labeling, Software

Journal: Advanced Science

Article Title: Phosphorylation of POU3F3 Mediated Nuclear Translocation Promotes Proliferation in Non‐Small Cell Lung Cancer through Accelerating ATP5PF Transcription and ATP Production

doi: 10.1002/advs.202411503

Figure Lengend Snippet: Knockout of POU3F3 downregulated intracellular ATP production by reducing ATP synthase activity. A) Relative ATP synthase activity in WT and POU3F3 knockout H1299 and A549 cells. B) Relative ATP production in WT and POU3F3 knockout H1299 and A549 cells treated with or without ATP synthase inhibitor oligomycin. C) Schematic of the distribution of ATP synthase complex, which are highlighted with different colors on ATP5PF and the subunit associated therewith ATP5 PB and ATP5PD. D) Flag pulldown and western blot to detect the interaction between ATP5PF and ATP5 PB and ATP5PD. E) Western blot detected the interaction between ATP5 PB and ATP5PD in WT and POU3F3 knockout cells.

Article Snippet: For complex V, used ATP5 PB (Abmart, PS18106S, 1:1000 dilution).

Techniques: Knock-Out, Activity Assay, Western Blot