atcc 43144 Search Results


93
ATCC streptococcus pasteurianus atcc 43144
Streptococcus Pasteurianus Atcc 43144, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
ATCC s gallolyticus subsp pasteurianus
Genetic organization of erm(T) resistance element and flanking regions in S. <t>gallolyticus</t> <t>subsp.</t> <t>pasteurianus</t> NTUH-7421. (A) Southern blot hybridization of erm(T) probe to S. gallolyticus subsp. pasteurianus NTUH-7421 genomic DNA after digestion with restriction enzymes ClaI, DdeI, HaeIII, HindII, and EcoRI (lanes 1 to 5, respectively). Lane M, DNA marker (digoxigenin-labeled DNA Molecular Weight Marker II′ [Roche]). (B) Genetic organization of erm(T) resistance element and flanking regions in S. gallolyticus subsp. pasteurianus NTUH-7421. Arrows represent putative open reading frames. The restriction sites are also shown.
S Gallolyticus Subsp Pasteurianus, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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Image Search Results


Genetic organization of erm(T) resistance element and flanking regions in S. gallolyticus subsp. pasteurianus NTUH-7421. (A) Southern blot hybridization of erm(T) probe to S. gallolyticus subsp. pasteurianus NTUH-7421 genomic DNA after digestion with restriction enzymes ClaI, DdeI, HaeIII, HindII, and EcoRI (lanes 1 to 5, respectively). Lane M, DNA marker (digoxigenin-labeled DNA Molecular Weight Marker II′ [Roche]). (B) Genetic organization of erm(T) resistance element and flanking regions in S. gallolyticus subsp. pasteurianus NTUH-7421. Arrows represent putative open reading frames. The restriction sites are also shown.

Journal:

Article Title: The erm (T) Gene Is Flanked by IS 1216V in Inducible Erythromycin-Resistant Streptococcus gallolyticus subsp. pasteurianus

doi: 10.1128/AAC.49.10.4347-4350.2005

Figure Lengend Snippet: Genetic organization of erm(T) resistance element and flanking regions in S. gallolyticus subsp. pasteurianus NTUH-7421. (A) Southern blot hybridization of erm(T) probe to S. gallolyticus subsp. pasteurianus NTUH-7421 genomic DNA after digestion with restriction enzymes ClaI, DdeI, HaeIII, HindII, and EcoRI (lanes 1 to 5, respectively). Lane M, DNA marker (digoxigenin-labeled DNA Molecular Weight Marker II′ [Roche]). (B) Genetic organization of erm(T) resistance element and flanking regions in S. gallolyticus subsp. pasteurianus NTUH-7421. Arrows represent putative open reading frames. The restriction sites are also shown.

Article Snippet: One erythromycin-susceptible reference strain (ATCC 43144) of S. gallolyticus subsp. pasteurianus and two isolates of erm (T)-negative S. gallolyticus subsp. pasteurianus [one was erythromycin resistant due to the presence of an erm (B) gene, and the other was erythromycin susceptible] were used as negative controls.

Techniques: Southern Blot, Hybridization, Marker, Labeling, Molecular Weight

Southern blot hybridization of erm(T) and IS1216V probe to S. gallolyticus subsp. pasteurianus strains. (A) Hybridization with an erm(T)-specific probe. Lanes 1 to 6 show erm(T)-positive isolates. Lane 1, NTUH-7421; lane 2, NTUH-8819; lane 3, NTUH-7499; lane 4, NTUH-3004; lane 5, NTUH-1043; lane 6, NTUH-4807; lanes 7 and 8, erm(T)-negative clinical isolates NTUH-1443 and NTUH-4046, respectively; lane 9, S. gallolyticus subsp. pasteurianus ATCC 43144; lane M, DNA marker (digoxigenin-labeled DNA Molecular Weight Marker II′ [Roche]). (B) Hybridization with IS1216V-specific probe. Lanes 1 to 6 and 8 to 13, erm(T)-positive isolates, as in lanes 1 to 6 in panel A. Lanes 7 and 14, erm(T)-negative clinical isolate NTUH-1443.

Journal:

Article Title: The erm (T) Gene Is Flanked by IS 1216V in Inducible Erythromycin-Resistant Streptococcus gallolyticus subsp. pasteurianus

doi: 10.1128/AAC.49.10.4347-4350.2005

Figure Lengend Snippet: Southern blot hybridization of erm(T) and IS1216V probe to S. gallolyticus subsp. pasteurianus strains. (A) Hybridization with an erm(T)-specific probe. Lanes 1 to 6 show erm(T)-positive isolates. Lane 1, NTUH-7421; lane 2, NTUH-8819; lane 3, NTUH-7499; lane 4, NTUH-3004; lane 5, NTUH-1043; lane 6, NTUH-4807; lanes 7 and 8, erm(T)-negative clinical isolates NTUH-1443 and NTUH-4046, respectively; lane 9, S. gallolyticus subsp. pasteurianus ATCC 43144; lane M, DNA marker (digoxigenin-labeled DNA Molecular Weight Marker II′ [Roche]). (B) Hybridization with IS1216V-specific probe. Lanes 1 to 6 and 8 to 13, erm(T)-positive isolates, as in lanes 1 to 6 in panel A. Lanes 7 and 14, erm(T)-negative clinical isolate NTUH-1443.

Article Snippet: One erythromycin-susceptible reference strain (ATCC 43144) of S. gallolyticus subsp. pasteurianus and two isolates of erm (T)-negative S. gallolyticus subsp. pasteurianus [one was erythromycin resistant due to the presence of an erm (B) gene, and the other was erythromycin susceptible] were used as negative controls.

Techniques: Southern Blot, Hybridization, Marker, Labeling, Molecular Weight

Localization of erm(T) on I-CeuI-generated chromosome fragments of S. gallolyticus subsp. pasteurianus isolates. (A) I-CeuI fragment restriction patterns separated by pulsed-field gel electrophoresis. (B) Hybridization with a probe specific for the erm(T) gene. (C) Hybridization with a 16S rRNA gene-specific probe. Lane M, molecular size standard (Saccharomyces cerevisiae chromosomal DNA). Lanes 1 to 6 show erm(T)-positive isolates. Lane 1, NTUH-7421; lane 2, NTUH-8819; lane 3, NTUH-7499; lane 4, NTUH-3004; lane 5, NTUH-1043; lane 6, NTUH-4807; lanes 7 and 8, erm(T)-negative clinical isolates NTUH-1443 and NTUH-4046, respectively.

Journal:

Article Title: The erm (T) Gene Is Flanked by IS 1216V in Inducible Erythromycin-Resistant Streptococcus gallolyticus subsp. pasteurianus

doi: 10.1128/AAC.49.10.4347-4350.2005

Figure Lengend Snippet: Localization of erm(T) on I-CeuI-generated chromosome fragments of S. gallolyticus subsp. pasteurianus isolates. (A) I-CeuI fragment restriction patterns separated by pulsed-field gel electrophoresis. (B) Hybridization with a probe specific for the erm(T) gene. (C) Hybridization with a 16S rRNA gene-specific probe. Lane M, molecular size standard (Saccharomyces cerevisiae chromosomal DNA). Lanes 1 to 6 show erm(T)-positive isolates. Lane 1, NTUH-7421; lane 2, NTUH-8819; lane 3, NTUH-7499; lane 4, NTUH-3004; lane 5, NTUH-1043; lane 6, NTUH-4807; lanes 7 and 8, erm(T)-negative clinical isolates NTUH-1443 and NTUH-4046, respectively.

Article Snippet: One erythromycin-susceptible reference strain (ATCC 43144) of S. gallolyticus subsp. pasteurianus and two isolates of erm (T)-negative S. gallolyticus subsp. pasteurianus [one was erythromycin resistant due to the presence of an erm (B) gene, and the other was erythromycin susceptible] were used as negative controls.

Techniques: Generated, Pulsed-Field Gel, Electrophoresis, Hybridization

Alignment of the leader peptide-encoding sequences of erm(T) in S. gallolyticus subsp. pasteurianus NTUH-7421, erm(GT) in Lactobacillus species (GenBank accession number M64090), and staphylococcal erm(C) (GenBank accession number M17990). A dash indicates an identical nucleotide and a dot indicates a gap. TAA (stop codon) is underlined.

Journal:

Article Title: The erm (T) Gene Is Flanked by IS 1216V in Inducible Erythromycin-Resistant Streptococcus gallolyticus subsp. pasteurianus

doi: 10.1128/AAC.49.10.4347-4350.2005

Figure Lengend Snippet: Alignment of the leader peptide-encoding sequences of erm(T) in S. gallolyticus subsp. pasteurianus NTUH-7421, erm(GT) in Lactobacillus species (GenBank accession number M64090), and staphylococcal erm(C) (GenBank accession number M17990). A dash indicates an identical nucleotide and a dot indicates a gap. TAA (stop codon) is underlined.

Article Snippet: One erythromycin-susceptible reference strain (ATCC 43144) of S. gallolyticus subsp. pasteurianus and two isolates of erm (T)-negative S. gallolyticus subsp. pasteurianus [one was erythromycin resistant due to the presence of an erm (B) gene, and the other was erythromycin susceptible] were used as negative controls.

Techniques: