Journal: Analytical and bioanalytical chemistry

Article Title: Microfluidic systems for studying dynamic function of adipocytes and adipose tissue

doi: 10.1007/s00216-017-0741-8

Figure Lengend Snippet: List of important adipokines in WAT *

Article Snippet: For example, several new hormones were identified as recently as 2016 [ 76 ], namely a new adipokine (asprosin) and two new batokines (Slit2-C and PM20D1).

Techniques: Control, Activity Assay

Journal: Analytical and bioanalytical chemistry

Article Title: Microfluidic systems for studying dynamic function of adipocytes and adipose tissue

doi: 10.1007/s00216-017-0741-8

Figure Lengend Snippet: Recent approaches for integrating adipose tissue or pre-differentiated cells onto microfluidic devices for dynamic functional studies

Article Snippet: For example, several new hormones were identified as recently as 2016 [ 76 ], namely a new adipokine (asprosin) and two new batokines (Slit2-C and PM20D1).

Techniques: Functional Assay, Fluorescence, Enzymatic Assay, Sampling, Enzyme-linked Immunosorbent Assay, Comparison, Extraction, Mass Spectrometry, Imaging

Journal: International Journal of Molecular Sciences

Article Title: Increased RBP4 and Asprosin Are Novel Contributors in Inflammation Process of Periodontitis in Obese Rats

doi: 10.3390/ijms242316739

Figure Lengend Snippet: Expression of periodontal and systemic inflammatory cytokines. ( A ) Relative inflammatory gene expression in gingiva, n = 5. ( B ) ELISA analysis of IL-6 in GCF (n = 4) and plasma (n = 6). ( C ) WB analysis of IL-6 protein expression in gingiva, n = 4. ( D ) Quantification of Western blot analysis. Protein content is expressed relative to the control and represents three independent experiments, with triplicate observations in each experiment. The volume is the sum of all pixel intensities within a band. All data are normalized to β-actin and are expressed as mean ± SEM. Different letters indicate statistical differences between groups.

Article Snippet: Meanwhile, plasma asprosin levels were measured using a rat asprosin ELISA kit (ER1944, FineTest, Wuhan, China).

Techniques: Expressing, Gene Expression, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Increased RBP4 and Asprosin Are Novel Contributors in Inflammation Process of Periodontitis in Obese Rats

doi: 10.3390/ijms242316739

Figure Lengend Snippet: Periodontium and systematic expression of adipokines in obesity and periodontitis. ( A ) Relative adipokine gene expression in gingiva, n = 5. ( B ) WB analysis (n = 4) of representative RBP4, asprosin and FBN1 protein expression in gingiva. ( C ) Quantification of Western blot analysis. Protein content is expressed relative to the control and represents three independent experiments with triplicate observations in each experiment. Volume is the sum of all pixel intensities within a band. All data are normalized to β-actin and are expressed as mean ± SEM. ( D ) ELISA analysis of asprosin in plasma and RBP4 in GCF and plasma, n = 6. ( E ) Representative immunohistochemistry images of periodontium tissue sections for asprosin and RBP4 from four groups. Positive cells are indicated by red arrowheads. ( F ) Quantification of asprosin and RBP4-positive cell of the four groups (data were collected from immunohistochemistry images of five individual rats in each group). Data are presented as mean ± SEM. Different letters indicate statistically significant differences among groups. Adipoq, adiponectin; Retn, resistin.

Article Snippet: Meanwhile, plasma asprosin levels were measured using a rat asprosin ELISA kit (ER1944, FineTest, Wuhan, China).

Techniques: Expressing, Gene Expression, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Increased RBP4 and Asprosin Are Novel Contributors in Inflammation Process of Periodontitis in Obese Rats

doi: 10.3390/ijms242316739

Figure Lengend Snippet: Correlation analysis between RBP4/asprosin and clinical and biomarker data in all participants.

Article Snippet: Meanwhile, plasma asprosin levels were measured using a rat asprosin ELISA kit (ER1944, FineTest, Wuhan, China).

Techniques: Biomarker Discovery

Journal: International Journal of Molecular Sciences

Article Title: Increased RBP4 and Asprosin Are Novel Contributors in Inflammation Process of Periodontitis in Obese Rats

doi: 10.3390/ijms242316739

Figure Lengend Snippet: Correlation analysis of RBP4 and asprosin with clinical attachment level and the bone-remodeling-related markers of the study groups. Partial regression plots of gingiva RBP4/asprosin mRNA expressions with the relative levels of clinical attachment level ( A ), RANKL ( B ) and Col-1 ( C ) mRNA expressions, respectively. All participants from the four groups (C, O, P and OP) were analyzed for correlation. (n = 20); the Pearson correlation analysis was used as the statistical test.

Article Snippet: Meanwhile, plasma asprosin levels were measured using a rat asprosin ELISA kit (ER1944, FineTest, Wuhan, China).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Increased RBP4 and Asprosin Are Novel Contributors in Inflammation Process of Periodontitis in Obese Rats

doi: 10.3390/ijms242316739

Figure Lengend Snippet: Periodontium and systematic expression of adipokines in obesity and periodontitis. ( A ) Relative adipokine gene expression in gingiva, n = 5. ( B ) WB analysis (n = 4) of representative RBP4, asprosin and FBN1 protein expression in gingiva. ( C ) Quantification of Western blot analysis. Protein content is expressed relative to the control and represents three independent experiments with triplicate observations in each experiment. Volume is the sum of all pixel intensities within a band. All data are normalized to β-actin and are expressed as mean ± SEM. ( D ) ELISA analysis of asprosin in plasma and RBP4 in GCF and plasma, n = 6. ( E ) Representative immunohistochemistry images of periodontium tissue sections for asprosin and RBP4 from four groups. Positive cells are indicated by red arrowheads. ( F ) Quantification of asprosin and RBP4-positive cell of the four groups (data were collected from immunohistochemistry images of five individual rats in each group). Data are presented as mean ± SEM. Different letters indicate statistically significant differences among groups. Adipoq, adiponectin; Retn, resistin.

Article Snippet: Later, the membranes or strips were incubated with each primary antibody: IL-1β (YT5201, Immunoway, Plano, TX, USA), RBP4 (11774-1-AP, Proteintech, Wuhan, China), asprosin (FNab09797, FineTest, Wuhan, China), fibrillin 1 (YT1684, FBN1, Immunoway, Plano, TX, USA) and β-actin (P30002F, Abmart, Shanghai, China) (all diluted 1:2000), overnight at 4 °C, and the second antibody (RS002, Immunoway, Plano, TX, USA, anti-rabbit, 1:10,000) in the ambient for 1 h after being washed with Tris-buffered saline and Tween (TBS-T).

Techniques: Expressing, Gene Expression, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Increased RBP4 and Asprosin Are Novel Contributors in Inflammation Process of Periodontitis in Obese Rats

doi: 10.3390/ijms242316739

Figure Lengend Snippet: Correlation analysis between RBP4/asprosin and clinical and biomarker data in all participants.

Article Snippet: Later, the membranes or strips were incubated with each primary antibody: IL-1β (YT5201, Immunoway, Plano, TX, USA), RBP4 (11774-1-AP, Proteintech, Wuhan, China), asprosin (FNab09797, FineTest, Wuhan, China), fibrillin 1 (YT1684, FBN1, Immunoway, Plano, TX, USA) and β-actin (P30002F, Abmart, Shanghai, China) (all diluted 1:2000), overnight at 4 °C, and the second antibody (RS002, Immunoway, Plano, TX, USA, anti-rabbit, 1:10,000) in the ambient for 1 h after being washed with Tris-buffered saline and Tween (TBS-T).

Techniques: Biomarker Discovery

Journal: International Journal of Molecular Sciences

Article Title: Increased RBP4 and Asprosin Are Novel Contributors in Inflammation Process of Periodontitis in Obese Rats

doi: 10.3390/ijms242316739

Figure Lengend Snippet: Correlation analysis of RBP4 and asprosin with clinical attachment level and the bone-remodeling-related markers of the study groups. Partial regression plots of gingiva RBP4/asprosin mRNA expressions with the relative levels of clinical attachment level ( A ), RANKL ( B ) and Col-1 ( C ) mRNA expressions, respectively. All participants from the four groups (C, O, P and OP) were analyzed for correlation. (n = 20); the Pearson correlation analysis was used as the statistical test.

Article Snippet: Later, the membranes or strips were incubated with each primary antibody: IL-1β (YT5201, Immunoway, Plano, TX, USA), RBP4 (11774-1-AP, Proteintech, Wuhan, China), asprosin (FNab09797, FineTest, Wuhan, China), fibrillin 1 (YT1684, FBN1, Immunoway, Plano, TX, USA) and β-actin (P30002F, Abmart, Shanghai, China) (all diluted 1:2000), overnight at 4 °C, and the second antibody (RS002, Immunoway, Plano, TX, USA, anti-rabbit, 1:10,000) in the ambient for 1 h after being washed with Tris-buffered saline and Tween (TBS-T).

Techniques:

Journal: Journal of Ovarian Research

Article Title: High levels of fatty acid-binding protein 5 excessively enhances fatty acid synthesis and proliferation of granulosa cells in polycystic ovary syndrome

doi: 10.1186/s13048-024-01368-6

Figure Lengend Snippet: qRT-PCR primers sequences

Article Snippet: FABP5 siRNA and negative control (NC) siRNA were synthesized by Guangzhou RiboBio Co., Ltd.

Techniques:

Journal: Journal of Ovarian Research

Article Title: High levels of fatty acid-binding protein 5 excessively enhances fatty acid synthesis and proliferation of granulosa cells in polycystic ovary syndrome

doi: 10.1186/s13048-024-01368-6

Figure Lengend Snippet: Aberrantly increased expression of FABP5 in GCs of patients and ovaries of mice with PCOS. A Bar plot showing FABP5 mRNA expression in the human primary ovarian GCs of PCOS patients and healthy individuals, as measured by qRT‒PCR ( n = 12). B Immunofluorescence staining of FABP5 in human primary ovarian GCs of PCOS patients and healthy individuals. Scale bar, 50 μm. C Bar plot showing the expression of Fabp5 mRNA in the ovaries of mice with PCOS measured by qRT‒PCR ( n = 6). D , E Immunoblot plot ( D ) and bar plot of the statistical analysis ( E ) showing the expression of FABP5 in the ovaries of mice with PCOS determined by Western blot analysis ( n = 4). F Immunohistochemical plot of FABP5 expression and localization in the ovaries of mice with PCOS. A two-tailed unpaired t test was used for all the statistical analyses in this section. ** P < 0.01

Article Snippet: FABP5 siRNA and negative control (NC) siRNA were synthesized by Guangzhou RiboBio Co., Ltd.

Techniques: Expressing, Immunofluorescence, Staining, Western Blot, Immunohistochemical staining, Two Tailed Test

Journal: Journal of Ovarian Research

Article Title: High levels of fatty acid-binding protein 5 excessively enhances fatty acid synthesis and proliferation of granulosa cells in polycystic ovary syndrome

doi: 10.1186/s13048-024-01368-6

Figure Lengend Snippet: Overexpression of FABP5 promotes fatty acid synthesis in KGN cells. A Bar plot showing the expression of FABP5 in KGN cells after transfection with the PCS2-myc- FABP5 or empty vector plasmids for 48 h, as determined via qRT‒PCR. B , C Immunoblotting plot ( B ) and bar plot of statistical analysis ( C ) showing the expression of FABP5 in KGN cells transfected with PCS2-myc- FABP5 or empty vector plasmids for 48 h. D Nile red staining of KGN cells after 48 h of FABP5 overexpression. The red signal indicates the formation of lipid droplets. All the samples were also stained with DAPI. Scale bar, 100 μm. E Statistical analysis of the number of lipid droplets. Five fields of view were randomly selected. F Bar plot showing the ACSL1 , GPAM1 , LPIN1 and DGAT2 mRNA expression in KGN cells after 48 h of FABP5 expression. A two-tailed unpaired t test was used for all the statistical analyses in this section. * P < 0.05, ** P < 0.01, *** P < 0.001. The groups transfected with the empty vector control or pCS2-myc- FABP5 plasmids were referred to as the EV-ctrl and myc- FABP5 groups, respectively

Article Snippet: FABP5 siRNA and negative control (NC) siRNA were synthesized by Guangzhou RiboBio Co., Ltd.

Techniques: Over Expression, Expressing, Transfection, Plasmid Preparation, Western Blot, Staining, Two Tailed Test, Control

Journal: Journal of Ovarian Research

Article Title: High levels of fatty acid-binding protein 5 excessively enhances fatty acid synthesis and proliferation of granulosa cells in polycystic ovary syndrome

doi: 10.1186/s13048-024-01368-6

Figure Lengend Snippet: Knockdown of FABP5 inhibits fatty acid synthesis in KGN cells. A Bar plot showing the expression of FABP5 in KGN cells after transfection with FABP5 or negative control siRNAs for 48 h. B , C Immunoblot plot ( B ) and bar plot of the statistical analysis ( C ) showing the expression of FABP5 in KGN cells transfected with FABP5 or negative control siRNAs for 48 h. D Nile red staining diagram of KGN cells after transfection with FABP5 siRNA for 48 h. The red signal indicates the formation of lipid droplets. All the samples were also stained with DAPI. Scale bar, 100 μm. E Statistical analysis of the number of lipid droplets. Five fields of view were randomly selected. F Bar plot showing ACSL1 , GPAM1 , LPIN1 and DGAT2 mRNA expression in KGN cells after transfection with FABP5 siRNA for 48 days. A two-tailed unpaired t test was used for all the statistical analyses in this section. * P < 0.05, ** P < 0.01, *** P < 0.001. The groups transfected with the negative control siRNA or with FABP5 siRNA were referred to as siNC and si FABP5 , respectively

Article Snippet: FABP5 siRNA and negative control (NC) siRNA were synthesized by Guangzhou RiboBio Co., Ltd.

Techniques: Knockdown, Expressing, Transfection, Negative Control, Western Blot, Staining, Two Tailed Test

Journal: Journal of Ovarian Research

Article Title: High levels of fatty acid-binding protein 5 excessively enhances fatty acid synthesis and proliferation of granulosa cells in polycystic ovary syndrome

doi: 10.1186/s13048-024-01368-6

Figure Lengend Snippet: FABP5 facilitates KGN cell proliferation. A Immunoblotting plot showing the expression of FABP5 and the cell proliferation marker PCNA in KGN cells after 48 h of FABP5 overexpression. B A line chart showing the proliferation of KGN cells after 48 h of FABP5 overexpression, as determined by CCK8 assays. C Immunofluorescence staining of Ki67 in KGN cells after 48 h of FABP5 overexpression. All the samples were also stained with DAPI. Scale bar, 100 μm. D Statistical analysis of the Ki67-positive cells. Five fields of view were randomly selected. E Immunoblotting plot showing the expression of FABP5 and PCNA in KGN cells after transfection with FABP5 or negative control siRNAs for 48 h. F Line chart showing the proliferation of KGN cells after transfection with FABP5 or negative control siRNAs for 48 h, as determined by CCK8 assays. G Immunofluorescence staining of Ki67 in KGN cells after transfection with FABP5 or negative control siRNAs for 48 h. All samples were also stained with DAPI. Scale bar, 100 μm. H Statistical analysis of the Ki67-positive cells. Five fields of view were randomly selected. A two-tailed unpaired t test was used for all the statistical analyses. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: FABP5 siRNA and negative control (NC) siRNA were synthesized by Guangzhou RiboBio Co., Ltd.

Techniques: Western Blot, Expressing, Marker, Over Expression, Immunofluorescence, Staining, Transfection, Negative Control, Two Tailed Test

Journal: Journal of Ovarian Research

Article Title: High levels of fatty acid-binding protein 5 excessively enhances fatty acid synthesis and proliferation of granulosa cells in polycystic ovary syndrome

doi: 10.1186/s13048-024-01368-6

Figure Lengend Snippet: FABP5 accelerates the proliferation of KGN cells by activating PI3K-AKT signaling. A Immunoblotting plot showing the expression of FABP5 and AKT and the phosphorylation of AKT in KGN cells after 48 h of FABP5 overexpression or knockdown. B , C Line chart showing the proliferation of KGN cells treated with 10 μM LY294002 or SC79 after 48 h of FABP5 overexpression or knockdown, as determined by CCK8 assays. * represents the comparison between the EV-ctrl and myc- FABP5 or siNC and siFABP5 groups; # represents the comparison between the myc- FABP5 and myc- FABP5 + LY294002 or si FABP5 and si FABP5 + SC79 groups; D , F Immunofluorescence staining of Ki67 in KGN cells treated with 10 μM LY294002 or SC79 for 48 h after FABP5 was overexpressed or knocked down. Scale bar, 100 μm. E , G Statistical analysis of Ki67-positive cells. Five fields of view were randomly selected. * P < 0.05, ** P < 0.01, *** P < 0.001. ## P < 0.01, ### P < 0.01

Article Snippet: FABP5 siRNA and negative control (NC) siRNA were synthesized by Guangzhou RiboBio Co., Ltd.

Techniques: Western Blot, Expressing, Phospho-proteomics, Over Expression, Knockdown, Comparison, Immunofluorescence, Staining

Journal: Journal of Ovarian Research

Article Title: High levels of fatty acid-binding protein 5 excessively enhances fatty acid synthesis and proliferation of granulosa cells in polycystic ovary syndrome

doi: 10.1186/s13048-024-01368-6

Figure Lengend Snippet: FABP5 accelerates fatty acid synthesis in KGN cells by activating PI3K-AKT signaling ( A , C ) Nile red staining of KGN cells treated with 10 μM LY294002 or SC79 for 48 h after 48 h of FABP5 overexpression or knockdown. The red signal indicates the formation of lipid droplets. All the samples were also stained with DAPI. Scale bar, 100 μm. B , D Statistical analysis of the number of lipid droplets. Five fields of view were randomly selected. E , G Bar plot showing ACSL1 , GPAM1 , LPIN1 and DGAT2 mRNA expression in KGN cells treated with 10 μM LY294002 or SC79 for 48 h after FABP5 overexpression or knockdown. A two-tailed unpaired t test was used for all the statistical analyses. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: FABP5 siRNA and negative control (NC) siRNA were synthesized by Guangzhou RiboBio Co., Ltd.

Techniques: Staining, Over Expression, Knockdown, Expressing, Two Tailed Test