Journal: Circulation Research

Article Title: Cardiac Na + Current Regulation by Pyridine Nucleotides

doi: 10.1161/circresaha.109.197277

Figure Lengend Snippet: Figure 1. The intracellular level of NADH is increased by A280V GPD1-L and is reversed by incubation with [NAD]o. Data from 3 to 4 samples are normalized to the SCN5A cell group. ***P0.001 vs all other groups.

Article Snippet: For biotin labeling, SCN5A cells were incubated as above for 2-10 min. Biotinylation of cell surface proteins was performed with the Pinpoint Cell Surface Protein Isolation Kit (Pierce, Rockford, IL) as previously described.3 For detection of SCN5A, the primary antibody (rabbit anti- SCN5A, Alomone Labs, Jerusalem, Israel) was diluted 1:100.

Techniques: Incubation

Journal: Circulation Research

Article Title: Cardiac Na + Current Regulation by Pyridine Nucleotides

doi: 10.1161/circresaha.109.197277

Figure Lengend Snippet: Figure 5. The effects of altering NAD(H) on arrhythmic risk. Representative MAP recordings with PES during Krebs–Henseleit buffer per- fusion (A), after 20 minutes perfusion with lactate:pyruvate (10:1) buffer to increase [NADH]i (B), and after reintroduction of Krebs–H- enseleit buffer (C). D, Representative traces of MAPs from left ventricular epicardium of Langendorff-perfused SCN5A/ heart during standard pacing at a basic cycle length of 125 ms in the control condition. Vertical lines below the MAPs represent the times when electric stimulations were delivered. E, Action potentials recorded with the patch-clamp technique in single ventricular myocytes and with the MAP electrode on whole heart. F, MAPs after 20 minutes of perfusion with 100 mol/L [NAD]o. G, Histogram of APD90 in control condition and with 100 mol/L [NAD]o. H, Representative MAPs recorded during PES showing PES-induced VT in SCN5A/

Article Snippet: For biotin labeling, SCN5A cells were incubated as above for 2-10 min. Biotinylation of cell surface proteins was performed with the Pinpoint Cell Surface Protein Isolation Kit (Pierce, Rockford, IL) as previously described.3 For detection of SCN5A, the primary antibody (rabbit anti- SCN5A, Alomone Labs, Jerusalem, Israel) was diluted 1:100.

Techniques: Control, Patch Clamp

Journal: Circulation Research

Article Title: Cardiac Na + Current Regulation by Pyridine Nucleotides

doi: 10.1161/circresaha.109.197277

Figure Lengend Snippet: Figure 6. Possible signaling pathways for the effects of A280V GPD1-L, [NADH]i and NAD to regulate the Nav1.5.

Article Snippet: For biotin labeling, SCN5A cells were incubated as above for 2-10 min. Biotinylation of cell surface proteins was performed with the Pinpoint Cell Surface Protein Isolation Kit (Pierce, Rockford, IL) as previously described.3 For detection of SCN5A, the primary antibody (rabbit anti- SCN5A, Alomone Labs, Jerusalem, Israel) was diluted 1:100.

Techniques: Protein-Protein interactions