artificial interstitial fluid Search Results


hcrt-1 orexin-a  (Bachem)
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Bachem hcrt-1 orexin-a
The number of tyrosine hydroxylase (TH)-ir neurons that were double-labeled with Fos-ir in the VTA in <t>artificial</t> <t>extracellular</t> <t>fluid</t> <t>(AECF)-treated</t> and hypocretin (HCRT)-treated animals. Presented are the number (mean + SEM) of TH-ir + Fos-ir neurons counted throughout the entire rostrocaudal extent of VTA (A), and within the four VTA subdivisions (B): medial-rostral (M-ROSTRAL); lateral-rostral (L-ROSTRAL); medial-caudal (M-CAUDAL); lateral-caudal (L-CAUDAL). (C) The number of large vs. small TH-ir neurons contained within the caudomedial VTA that were double-labeled for Fos-ir. HCRT produced a robust increase in the number of TH-ir neurons expressing Fos-ir relative to AECF-treated animals (A). The HCRT-induced increase in Fos-ir + TH-ir double-labeling was largely restricted to the caudomedial VTA (M-CAUDAL, B), particularly among small DA neurons (C). *P < 0.05.
Hcrt 1 Orexin A, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The number of tyrosine hydroxylase (TH)-ir neurons that were double-labeled with Fos-ir in the VTA in artificial extracellular fluid (AECF)-treated and hypocretin (HCRT)-treated animals. Presented are the number (mean + SEM) of TH-ir + Fos-ir neurons counted throughout the entire rostrocaudal extent of VTA (A), and within the four VTA subdivisions (B): medial-rostral (M-ROSTRAL); lateral-rostral (L-ROSTRAL); medial-caudal (M-CAUDAL); lateral-caudal (L-CAUDAL). (C) The number of large vs. small TH-ir neurons contained within the caudomedial VTA that were double-labeled for Fos-ir. HCRT produced a robust increase in the number of TH-ir neurons expressing Fos-ir relative to AECF-treated animals (A). The HCRT-induced increase in Fos-ir + TH-ir double-labeling was largely restricted to the caudomedial VTA (M-CAUDAL, B), particularly among small DA neurons (C). *P < 0.05.

Journal: The European journal of neuroscience

Article Title: Hypocretin/orexin preferentially activates caudomedial ventral tegmental area dopamine neurons

doi: 10.1111/j.1460-9568.2008.06453.x

Figure Lengend Snippet: The number of tyrosine hydroxylase (TH)-ir neurons that were double-labeled with Fos-ir in the VTA in artificial extracellular fluid (AECF)-treated and hypocretin (HCRT)-treated animals. Presented are the number (mean + SEM) of TH-ir + Fos-ir neurons counted throughout the entire rostrocaudal extent of VTA (A), and within the four VTA subdivisions (B): medial-rostral (M-ROSTRAL); lateral-rostral (L-ROSTRAL); medial-caudal (M-CAUDAL); lateral-caudal (L-CAUDAL). (C) The number of large vs. small TH-ir neurons contained within the caudomedial VTA that were double-labeled for Fos-ir. HCRT produced a robust increase in the number of TH-ir neurons expressing Fos-ir relative to AECF-treated animals (A). The HCRT-induced increase in Fos-ir + TH-ir double-labeling was largely restricted to the caudomedial VTA (M-CAUDAL, B), particularly among small DA neurons (C). *P < 0.05.

Article Snippet: Animals received a 2-μL infusion of artificial extracellular fluid (AECF) or 0.5 nmol HCRT-1 (Orexin-A; Bachem, King of Prussia, PA, USA; dissolved in AECF).

Techniques: Labeling, Produced, Expressing

TH-ir + Fos-ir double-labeling: cell counts and percentage TH-ir

Journal: The European journal of neuroscience

Article Title: Hypocretin/orexin preferentially activates caudomedial ventral tegmental area dopamine neurons

doi: 10.1111/j.1460-9568.2008.06453.x

Figure Lengend Snippet: TH-ir + Fos-ir double-labeling: cell counts and percentage TH-ir

Article Snippet: Animals received a 2-μL infusion of artificial extracellular fluid (AECF) or 0.5 nmol HCRT-1 (Orexin-A; Bachem, King of Prussia, PA, USA; dissolved in AECF).

Techniques: