arthrobacter sp strain atcc 33790 Search Results


arthrobacter sp strain atcc 33790  (ATCC)
94
ATCC arthrobacter sp strain atcc 33790
Arthrobacter Sp Strain Atcc 33790, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
arthrobacter sp strain atcc 33790 - by Bioz Stars, 2026-04
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pentachlorophenol degradation  (ATCC)
94
ATCC pentachlorophenol degradation
Pentachlorophenol Degradation, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
pentachlorophenol degradation - by Bioz Stars, 2026-04
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cd8a (kt15)-fitc  (Proimmune)
90
Proimmune cd8a (kt15)-fitc
PBMCs derived from an HLA-A*02:01 patient with high serum level of anti-CMV Ab were pulsed with the peptides in . The level of IFN-γ secreted by activated <t>CD8+</t> T cells was detected by ELISpot assay. The dotted line indicates the noise level coming from unspecific activation of CTL in the negative control CMV resembling tumor (T1–5) and viral (V1–5) peptides presented in (A) . PBMCs derived from an HLA-A*02:01 positive patient with high serum level of anti-CMV Ab and from a healthy donor (HD) found positive for CMV response were tested for anti-CMV response by ELISpot assay using CMV-specific HLA-A*02:01 restricted peptide NLVPMVATV. P value was calculated using t-test with Mann-Whitney correction (B) . Experimental design for cross-reactivity assay. On day 0 primary human PBMCs were in vitro stimulated with matching viral and tumor peptides, on day 2 IL2 was added to the culture in order to sustain the primary cell culture until day 8 when GolgiStop was added. After inhibition by GolgiStop on day 9 the IFN-γ positive and negative fractions of CD8 T cells were sorted using FACS for further TCRβ sequencing (C) . Venn diagrams indicating the number of shared clones between viral or tumor antigen expanded active CD8+ T-cells and the baseline CD8+ T-cells. Clones that expanded with IL2 stimulation only were excluded from further analysis (D&E) . The top three clones with highest combined productive frequency among IFN-γ producing CD8+ T cell pool that overlapped between viral and tumor antigen expansion, but were not expanded with IL2 stimulation only. Bars represent the productive frequency of clones in IFN-γ producing (pink) and IFN-γ negative (grey) CD8+ T-cells after viral (striped fill) and tumor (solid fill) antigen expansion. Black dotted line represents the baseline expression (black bar) (F&G) .
Cd8a (Kt15) Fitc, supplied by Proimmune, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd8a (kt15)-fitc/product/Proimmune
Average 90 stars, based on 1 article reviews
cd8a (kt15)-fitc - by Bioz Stars, 2026-04
90/100 stars
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PBMCs derived from an HLA-A*02:01 patient with high serum level of anti-CMV Ab were pulsed with the peptides in . The level of IFN-γ secreted by activated CD8+ T cells was detected by ELISpot assay. The dotted line indicates the noise level coming from unspecific activation of CTL in the negative control CMV resembling tumor (T1–5) and viral (V1–5) peptides presented in (A) . PBMCs derived from an HLA-A*02:01 positive patient with high serum level of anti-CMV Ab and from a healthy donor (HD) found positive for CMV response were tested for anti-CMV response by ELISpot assay using CMV-specific HLA-A*02:01 restricted peptide NLVPMVATV. P value was calculated using t-test with Mann-Whitney correction (B) . Experimental design for cross-reactivity assay. On day 0 primary human PBMCs were in vitro stimulated with matching viral and tumor peptides, on day 2 IL2 was added to the culture in order to sustain the primary cell culture until day 8 when GolgiStop was added. After inhibition by GolgiStop on day 9 the IFN-γ positive and negative fractions of CD8 T cells were sorted using FACS for further TCRβ sequencing (C) . Venn diagrams indicating the number of shared clones between viral or tumor antigen expanded active CD8+ T-cells and the baseline CD8+ T-cells. Clones that expanded with IL2 stimulation only were excluded from further analysis (D&E) . The top three clones with highest combined productive frequency among IFN-γ producing CD8+ T cell pool that overlapped between viral and tumor antigen expansion, but were not expanded with IL2 stimulation only. Bars represent the productive frequency of clones in IFN-γ producing (pink) and IFN-γ negative (grey) CD8+ T-cells after viral (striped fill) and tumor (solid fill) antigen expansion. Black dotted line represents the baseline expression (black bar) (F&G) .

Journal: medRxiv

Article Title: Viral Molecular Mimicry Influences the Antitumor Immune Response in Murine and Human Melanoma

doi: 10.1101/2020.09.09.20191171

Figure Lengend Snippet: PBMCs derived from an HLA-A*02:01 patient with high serum level of anti-CMV Ab were pulsed with the peptides in . The level of IFN-γ secreted by activated CD8+ T cells was detected by ELISpot assay. The dotted line indicates the noise level coming from unspecific activation of CTL in the negative control CMV resembling tumor (T1–5) and viral (V1–5) peptides presented in (A) . PBMCs derived from an HLA-A*02:01 positive patient with high serum level of anti-CMV Ab and from a healthy donor (HD) found positive for CMV response were tested for anti-CMV response by ELISpot assay using CMV-specific HLA-A*02:01 restricted peptide NLVPMVATV. P value was calculated using t-test with Mann-Whitney correction (B) . Experimental design for cross-reactivity assay. On day 0 primary human PBMCs were in vitro stimulated with matching viral and tumor peptides, on day 2 IL2 was added to the culture in order to sustain the primary cell culture until day 8 when GolgiStop was added. After inhibition by GolgiStop on day 9 the IFN-γ positive and negative fractions of CD8 T cells were sorted using FACS for further TCRβ sequencing (C) . Venn diagrams indicating the number of shared clones between viral or tumor antigen expanded active CD8+ T-cells and the baseline CD8+ T-cells. Clones that expanded with IL2 stimulation only were excluded from further analysis (D&E) . The top three clones with highest combined productive frequency among IFN-γ producing CD8+ T cell pool that overlapped between viral and tumor antigen expansion, but were not expanded with IL2 stimulation only. Bars represent the productive frequency of clones in IFN-γ producing (pink) and IFN-γ negative (grey) CD8+ T-cells after viral (striped fill) and tumor (solid fill) antigen expansion. Black dotted line represents the baseline expression (black bar) (F&G) .

Article Snippet: Antibodies used in this study were the following: TruStain fcX (anti-mouse CD16/32) (Biolegend - 101320), CD3-BV711 (BD - 563123), CD4-PECF594 (BD - 562285), CXCR3-APC (BD - 562266), CD44-V450 (BD - 560451), Ki67-PECy7 (BD - 561283), CD8a (KT15)-FITC (Proimmune 1705F/33790).

Techniques: Derivative Assay, Enzyme-linked Immunospot, Activation Assay, Negative Control, MANN-WHITNEY, In Vitro, Cell Culture, Inhibition, Sequencing, Clone Assay, Expressing