Journal: Journal of Clinical Laboratory Analysis

Article Title: Super‐enhancer–associated long noncoding RNA RP11‐569A11.1 inhibited cell progression and metastasis by regulating IFIT2 in colorectal cancer

doi: 10.1002/jcla.23780

Figure Lengend Snippet: SE‐LncRNA microarray analysis of CRC tissues. A, The scatter plot displayed variation in SE‐LncRNA expression between group‐N (normal) and group‐T (CRC). B, The volcano plot represented the fold change values and P‐values of the microarray data. C, Hierarchical clustering revealed differentially expressed SE‐LncRNAs (fold change >2, p ‐value <0.05). Red and green colors represented upregulated and downregulated SE‐LncRNAs, respectively

Article Snippet: Given that the expression abundance of SE‐LncRNA at lower levels than that of mRNA, and RNA‐seq is not sensitive to low‐abundance SE‐LncRNAs, we conducted Arraystar human SE‐LncRNAs microarray to detect differentially expressed SE‐LncRNAs.

Techniques: Microarray, Expressing

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: LINC01278 Sponges miR‐500b‐5p to Regulate the Expression of ACTG2 to Control Phenotypic Switching in Human Vascular Smooth Muscle Cells During Aortic Dissection

doi: 10.1161/JAHA.120.018062

Figure Lengend Snippet: A , miRNA expression profile. The green plots are decreased and red plots are upregulated differentially expressed genes. B , Heat map of miRNA profile. C , Quantitative reverse transcription‐PCR validation of miRNA microarray. D , lncRNA expression profile. E , Heat map of lncRNA profile. F , Quantitative reverse transcription‐PCR validation of lncRNA microarray. AD indicates aortic dissection; and qPCR, quantitative reverse transcription–polymerase chain reaction. ** P <0.01.

Article Snippet: All the RNAs (6 AD and normal control samples) were extracted using TRIzol, quantified (for integrity and concentration) using NanoDrop ND‐1000, and analyzed using the Arraystar Human miRNA and lncRNA microarray.

Techniques: Expressing, Reverse Transcription, Biomarker Discovery, Microarray, Dissection, Polymerase Chain Reaction

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: LINC01278 Sponges miR‐500b‐5p to Regulate the Expression of ACTG2 to Control Phenotypic Switching in Human Vascular Smooth Muscle Cells During Aortic Dissection

doi: 10.1161/JAHA.120.018062

Figure Lengend Snippet: A , miRNA profile and miRNA predicts by TargetScan online tool. B , lncRNA profile and lncRNA predictions by DIANA online tool. C , The interactive network of lncRNA‐miRNA‐mRNA. There were 13 co‐expressed miRNAs and 5 mRNAs and 6 lncRNAs related in the vascular smooth muscle contraction pathway in the network. The red triangle represents mRNAs, purple diamond represents miRNAs, and blue circle represents lncRNAs. Solid lines represent relationship between 2 nodes.

Article Snippet: All the RNAs (6 AD and normal control samples) were extracted using TRIzol, quantified (for integrity and concentration) using NanoDrop ND‐1000, and analyzed using the Arraystar Human miRNA and lncRNA microarray.

Techniques:

Journal: BMC Oral Health

Article Title: Identification of key module and hub genes in pulpitis using weighted gene co-expression network analysis

doi: 10.1186/s12903-022-02638-9

Figure Lengend Snippet: Summary of two data sets of pulpitis

Article Snippet: GSE92681 including 7 pulpitis and 5 healthy pulp samples from human, using the microarray platform GPL16956 (Agilent045997 Arraystar Human LncRNA Micro-array V3).

Techniques: Quantitative Proteomics, Gene Expression

Journal: Journal of Cellular and Molecular Medicine

Article Title: A novel antisense lncRNA NT5E promotes progression by modulating the expression of SYNCRIP and predicts a poor prognosis in pancreatic cancer

doi: 10.1111/jcmm.15718

Figure Lengend Snippet: LncNT5E is up‐regulated in pancreatic cancer (PC) tissues and cell lines. A, The heat map from our previous lncRNA microarray reflected the differentially expressed lncRNAs in PC and normal tissues. T represents PC tissue, and N represents normal pancreatic tissue. ENST00000421594 indicates lncNT5E. B, Relative expression of lncNT5E in 45 paired PC and adjacent normal tissues. LncNT5E expression from all tissues was normalized to 18S expression (ΔCT) and then compared with adjacent normal tissues and converted to the fold change (2 −ΔΔCT ). C, Relative expression of lncNT5E in different cell lines. Data are shown as fold change (2 −ΔΔCT ). * P < .05, ** P < .01, *** P < .001

Article Snippet: We used microarray detection (H1602063, Arraystar Human LncRNA 8 × 60 k v3.0 1‐color) to study lncRNAs in three pairs of PC and adjacent normal tissues.

Techniques: Microarray, Expressing