Journal: Molecular BioSystems

Article Title: Elucidating role of salivary proteins in denture stomatitis using a proteomic approach

doi: 10.1039/c2mb25283j

Figure Lengend Snippet: Fig. 4 Differential fold expression of 4 masses identified by SELDI-TOF/ MS in four different Protein Chip arrays; (A) H50 array with 5283 kDa mass, (B) Q10 array with 5227 kDa mass, (C) IMAC30 array with 12679 kDa mass, and (D) CM10 array with 13274 kDa mass.

Article Snippet: Materials for SELDI-TOF/MS analysis included Denaturant (9 M urea, 2% CHAPS), Bio-Rad ProteinChip CM10 arrays, Bio-Rad ProteinChip H50 arrays, Bio-Rad ProteinChip IMAC30 arrays, Bio-Rad ProteinChip Q10 arrays, and Bio-Rad SPA matrix.

Techniques: Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Induction of Apoptosis and Modulation of Caspase Activity on MCF-7 Human Breast Cancer Cells by Bioactive Fractionated Cocoa Leaf Extract

doi: 10.31557/APJCP.2023.24.7.2473

Figure Lengend Snippet: CL Bioactive Fraction (II-F7) Induce Apoptosis in MCF-7 Cells. (A) flow cytometry profile of MCF-7 cells treated with three concentrations (IC 25 , IC 50 , IC 75 ) of CL bioactive fraction (II-F7) for 48 h and stained with propidium iodide (PI). (B) percentage of MCF-7 cells undergoing late and early apoptosis. (C) levels of Caspase-3, -6, and -9 in MCF-7 cells after 48 h of treatment with three concentrations (IC 25 , IC 50 , IC75) of CL bioactive fraction (II-F7). (D) Apoptotic DNA fragmentation in MCF-7 cells following 48 hours treatment with CL bioactive fraction (II-F7) at concentrations of IC25, IC50 and IC75. L1: DNA size marker, L2: untreated cells (control), L3: IC 25 , L4: IC 50 , L5: IC 75 . Values are the means of triplicate samples (n = 3). Data are presented as the mean ± SEM. Superscript letters indicate significant difference according to One-way ANOVA and Tukey honest significant difference test (P<0.05).

Article Snippet: Expression of apoptotic genes in MCF-7 cells The Human Apoptosis ExprofileTM qPCR Arrays (GeneCopoeia, USA) was used according to the manufacturer guideline to study the gene expression of apoptosis-related genes in MCF-7 cells treated with CL bioactive fraction (II-F7) at IC 50 and IC 75 .

Techniques: Flow Cytometry, Staining, Marker, Control

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Induction of Apoptosis and Modulation of Caspase Activity on MCF-7 Human Breast Cancer Cells by Bioactive Fractionated Cocoa Leaf Extract

doi: 10.31557/APJCP.2023.24.7.2473

Figure Lengend Snippet: CL Bioactive Fraction (II-F7) Inhibit MCF-7 Proliferation. (A) Cell viability percentage of MCF-7 cells-treated with serial concentrations of CL bioactive fraction (II-F7) at 24 h, 48 h, and 72 h. (B) phase contrast micrograph of light and fluorescence microscope for MCF-7 cells treated with three concentrations (IC 25 , IC 50 , IC 75 ) of CL bioactive fraction (II-F7) for 48 h. Under light microscope, the treated cells show apoptotic bodies (circle), cell shrinkage (arrow) and membrane blebbing (head arrow). The fluorescence micrographs show untreated cells with normal structure (intact green) while the treated cells show early apoptosis (bright green), late apoptosis (dark orange) and necrotic cells (bright red). VC: viable cells, EAC: early apoptotic cells, LAC: late apoptotic cells, NC: necrotic cells. Figures were obtained from three independent experiments with similar parameters. (C) Percentage of viable, apoptotic, and necrotic of MCF-7 cells following 48 hours treatment with CL sub-fraction (II/SF7) at concentrations of IC 25 , IC 50 and IC 75 . (D) Ψ∆m changes in MCF-7 cells treated with three concentrations of CL bioactive fraction (II-F7) for 48 h and stained with JC-1. Values are the means of triplicate samples (n = 3). Data are presented as the mean ± SEM. Superscript letters indicate significant difference according to One-way ANOVA and Tukey honest significant difference test (P<0.05).

Article Snippet: Expression of apoptotic genes in MCF-7 cells The Human Apoptosis ExprofileTM qPCR Arrays (GeneCopoeia, USA) was used according to the manufacturer guideline to study the gene expression of apoptosis-related genes in MCF-7 cells treated with CL bioactive fraction (II-F7) at IC 50 and IC 75 .

Techniques: Fluorescence, Microscopy, Light Microscopy, Membrane, Staining

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Induction of Apoptosis and Modulation of Caspase Activity on MCF-7 Human Breast Cancer Cells by Bioactive Fractionated Cocoa Leaf Extract

doi: 10.31557/APJCP.2023.24.7.2473

Figure Lengend Snippet: GPR Fold Change of Selected Apoptosis-Related Gene Expression in MCF-7 Cells Treated with CL Bioactive Fraction (II-F7). Bold-face and italic indicates up-regulated and down-regulate of the selected gene expression in comparison with IC 50 and IC 75 concentration. Data represent the mean of samples induced fold change in gene expression relative to control-treated cells (n=3), P < 0.05

Article Snippet: Expression of apoptotic genes in MCF-7 cells The Human Apoptosis ExprofileTM qPCR Arrays (GeneCopoeia, USA) was used according to the manufacturer guideline to study the gene expression of apoptosis-related genes in MCF-7 cells treated with CL bioactive fraction (II-F7) at IC 50 and IC 75 .

Techniques: Gene Expression, Comparison, Concentration Assay, Control

Journal: MedComm

Article Title: Dormant Metastases Exhibit a Unique Phenotype Primarily Promoted by the Ch25h Gene and Are Maintained in Dormancy by T Lymphocytes

doi: 10.1002/mco2.70437

Figure Lengend Snippet: Genes differentially expressed in the dormant metastasis group. Heat maps are presented to illustrate the differential expression data between the three metastatic groups obtained from RNA‐Seq (A), or RT‐qPCR in genes involved in the cholesterol synthesis pathway (B), in chemokine genes (C), and in surface marker genes (D). Only genes that displayed a greater than twofold difference in expression (log2>1) with a p ‐value less than 0.001 were identified and plotted in the heatmap. The expression values are presented as log2 values and are scaled by gene. In RT‐qPCR assays, the expression levels of the genes of interest were determined with respect to the levels of the β‐actin and GAPDH housekeeping genes. The data for the overt‐met group were set to 1. The values represent the means of three independent experiments performed in duplicate. The statistical analysis was performed using ANOVA, followed by Tukey's post hoc test.

Article Snippet: The miProfileMouse miRNome miRNA qPCR Array (catalog QM002; GeneCopoeia) was used to analyze 834 mouse miRNAs.

Techniques: Quantitative Proteomics, RNA Sequencing, Quantitative RT-PCR, Marker, Expressing

Journal: MedComm

Article Title: Dormant Metastases Exhibit a Unique Phenotype Primarily Promoted by the Ch25h Gene and Are Maintained in Dormancy by T Lymphocytes

doi: 10.1002/mco2.70437

Figure Lengend Snippet: MicroRNAs that are differentially expressed in the dormant metastasis group. (A) Heat map is presented to illustrate the differential expression data between the three metastatic groups. (B) miRNA enrichment analysis of miRNAs differentially expressed in dormant‐met group (miEAA). The expression of 834 miRNAs was measured using the ‘mouse miRNome qPCR arrays 18.0’ (Genecopoeia). Only miRNAs that displayed a greater than twofold difference ( p < 0.01) when comparing the dormant‐met group with the nude‐met and overt‐met groups were plotted. The expression levels of the miRNAs of interest were determined with respect to the levels of six housekeeping snRNAs (MK1‐MK6). The data for the overt‐met group were set to 1. The values are presented as the means of three independent experiments, each performed in duplicate. The statistical analysis was conducted using an ANOVA test, followed by a Tukey's post hoc test.

Article Snippet: The miProfileMouse miRNome miRNA qPCR Array (catalog QM002; GeneCopoeia) was used to analyze 834 mouse miRNAs.

Techniques: Quantitative Proteomics, Expressing