Journal: BMC Biotechnology

Article Title: Low density DNA microarray for detection of most frequent TP53 missense point mutations

doi: 10.1186/1472-6750-5-8

Figure Lengend Snippet: Detection of TP53 silent and missense base substitutions using DNA sequencing versus DNA microarray hybridization.

Article Snippet: In the present work we designed a novel small format DNA chip (genosensor array) to search for nine point mutations in codons 248, 249 and 273 of the TP53 gene.

Techniques: DNA Sequencing, Microarray, Hybridization, Sequencing, Mutagenesis

Journal: BMC Biotechnology

Article Title: Low density DNA microarray for detection of most frequent TP53 missense point mutations

doi: 10.1186/1472-6750-5-8

Figure Lengend Snippet: Layout of the TP53 low density DNA microarray . (A): Names and alignment of stacking oligonucleotides and probes to their respective synthetic wild type target sequence. Bold letters correspond to the nucleotide change in DNA sequence due to the point mutations. (B): Layout of the probe array on the glass slide. The probes were applied to the slide in triplicate as depicted at the top. The numbers correspond to the probes in Table 1.

Article Snippet: In the present work we designed a novel small format DNA chip (genosensor array) to search for nine point mutations in codons 248, 249 and 273 of the TP53 gene.

Techniques: Microarray, Sequencing