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Thermo Fisher
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Transient overexpression of ARPP19 NM 006628 in HEK293T cells paraffin embedded 4 um sections controls for ICC IHC staining 25 slides per pack
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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Characterization of Mast2 kinase defines structural features, regulation, and substrates
doi: 10.1016/j.jbc.2025.110922
Figure Lengend Snippet: Mast2 (294–1197) robustly phosphorylates S67-ENSA. A, the site-specific information of selected significantly upregulated peptides from B ( green box ). B, nonradiolabeled substrate assay of ENSA (MCE) phosphorylated by Mast2 (294–1197). Total ENSA and FLAG immunoblot are shown with a representative pS67-ENSA blot ( right ), and phosphorylation was quantified by percent maximal AU with each aligned data point shown over a nonlinear curve determination ( left ). K m was calculated using GraphPad Prism. MAST, microtubule-associated serine/threonine; ENSA, endosulfine-α.
Article Snippet: Primary antibodies for total AKT (2920S), phospho-specific AKT (pT308, 9275S and pS473, 4060L), phospho-specific S6K (pT389, 9205S), phospho-specific
Techniques: Western Blot, Phospho-proteomics
Journal: Quantitative Biology
Article Title: Metabolic‐immune interactions in gastric cancer T cells: A single‐cell atlas for prognostic biomarker identification
doi: 10.1002/qub2.70027
Figure Lengend Snippet: RT‐qPCR showing the mRNA expression of (A) ZNF207 , (B) ZNRF1 , (C) RGS1 , (D) ARPP19 , (E) CXCR4 , and (F) CTLA4 in paracancerous (principal component, PC) tissues ( n = 10) and gastric cancer (GC) samples ( n = 10). * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Immunohistochemical staining was then carried out using primary antibodies targeting RGS1 (Sigma, HPA028453), CXCR4 (Cell Signaling Technology, #97680), CTLA4 (Cell Signaling Technology, #53560),
Techniques: Quantitative RT-PCR, Expressing
Journal: Quantitative Biology
Article Title: Metabolic‐immune interactions in gastric cancer T cells: A single‐cell atlas for prognostic biomarker identification
doi: 10.1002/qub2.70027
Figure Lengend Snippet: Immunohistochemical staining of target genes (A) ZNF207 , (B) ARPP19 , (C) ZNRF1 , (D) CXCR4 , (E) RGS1 , and (F) CTLA4 expression profiles in gastric cancer specimens and their corresponding adjacent normal gastric tissues. Brown chromogenic staining reveals positive protein expression. Blue counterstaining visualizes cell nuclei. Scale bar: 100 μm.
Article Snippet: Immunohistochemical staining was then carried out using primary antibodies targeting RGS1 (Sigma, HPA028453), CXCR4 (Cell Signaling Technology, #97680), CTLA4 (Cell Signaling Technology, #53560),
Techniques: Immunohistochemical staining, Staining, Expressing
Journal: International Journal of Molecular Sciences
Article Title: IGFL2-AS1, a Long Non-Coding RNA, Is Associated with Radioresistance in Colorectal Cancer
doi: 10.3390/ijms24020978
Figure Lengend Snippet: AKT pathway signaling is suppressed by sh-IGFL2-AS1. ( A ) Schematic depicting the IGFL2-AS1 pathway. ( B ) Relative transcription of ARPP19, measured using qRT PCR. The graph depicts the levels of ARPP19 in the indicated cell lines. Data are normalized to those of the sh-NC-HT29 cell line; error bars indicate SDs. *** p < 0.001. ( C ) Protein expression of ARPP19, measured by western blotting. The expression of β-actin was used as a loading control. ( D ) Western blot analyses of various proteins downstream of AKT. Cells incubated as indicated after irradiation (3 Gy) were lysed and analyzed by western blotting; β-actin was used as a loading control. ( E – H ) Densitometric measurements of protein expressions, corresponding to ( D ). The results are presented as the ratio of phosphor-AKT/AKT ( E ), cyclin D1/β-actin ( F ), c-Myc/β-actin ( G ), and E-cadherin/β-actin ( H ).
Article Snippet:
Techniques: Quantitative RT-PCR, Expressing, Western Blot, Control, Incubation, Irradiation