Journal: Immunity

Article Title: Sepsis expands a CD39 + plasmablast population that promotes immunosuppression via adenosine-mediated inhibition of macrophage antimicrobial activity.

doi: 10.1016/j.immuni.2021.08.005

Figure Lengend Snippet: Figure 2. CD39 is required for sepsis-induced immunosuppression (A and B) Splenic cells from CLP-surviving mice were harvested 15 days after CLP. (A) Histogram and mean fluorescence intensity (MFI) of CD39 expression in total splenic cells. n = 6.

Article Snippet: For the detection of the extracellular ATP, we performed CD19+ B cell culture stimulated with IL-4 (10 ng.mL-1, R&D Systems) for 6 h in the presence of a CD39 inhibitor (ARL 67156 trisodium salt, 200 mM, Tocris), and culture supernatants were collected and measured for ATP levels 6 h later.

Techniques: Expressing

Journal: Immunity

Article Title: Sepsis expands a CD39 + plasmablast population that promotes immunosuppression via adenosine-mediated inhibition of macrophage antimicrobial activity.

doi: 10.1016/j.immuni.2021.08.005

Figure Lengend Snippet: Figure 4. CD39+ B cells promote immunosuppression in sepsis-surviving mice (A–C) Splenic CD19+ B cells were isolated from CLP-surviving mice 15 days after CLP. (A) mRNA expression of Entpd1. n = 5. (B) Quantification of ecto-ATPase activity with or without CD39i. n = 8–9. (C) Concentration of adenosine in the cell culture supernatant. n = 6. (D) Schematic of B cell transfer into Rag1/ mice following by sepsis-induced immunosuppression model (E–G) (see also STAR Methods). (E) Flow cytometry analysis of CD19+ splenic cells from WT, Rag1/, and Rag1/ B cell (B cell transferred) mice 15 days after CLP. n = 5–7. (F) Plasma adenosine concentrations from sepsis survivors 15 days after CLP. n = 5–7.

Article Snippet: For the detection of the extracellular ATP, we performed CD19+ B cell culture stimulated with IL-4 (10 ng.mL-1, R&D Systems) for 6 h in the presence of a CD39 inhibitor (ARL 67156 trisodium salt, 200 mM, Tocris), and culture supernatants were collected and measured for ATP levels 6 h later.

Techniques: Isolation, Expressing, Activity Assay, Concentration Assay, Cell Culture, Flow Cytometry, Clinical Proteomics