Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 1 Abnormally high expression of ARPC5 in glioma. (A) Differential expression of ARPC5 in various tumor types was analyzed based on the TIMER online website. (B) The distinct upregulation of ARPC5 in glioma was demonstrated in GEPIA online website. (C) Box plot based on the expression of ARPC5 in the GSE2223 (Glioma = 50, Normal = 4). (D) Box plot based on the expression of ARPC5 in the GSE29796 (Glioma = 52, Normal = 20). (E) Box plot based on the expression of ARPC5 in the GSE116520 (Glioma = 34, Normal = 8). (F) Box plot based on the protein expression of ARPC5 in the CPTAC samples (Glioma = 99, Normal = 10). (G) ARPC5 protein expression was detected in cerebral cortex (Patient id: 1582), low grade glioma (Patient id: 3365), and high-grade glioma (Patient id: 3251) tissues from HPA dataset. (H) Representative images of ARPC5 expression in glioma and its surrounding tissues. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing, Quantitative Proteomics

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 2 Correlation between different status of ARPC5 expression and prognosis of glioma patients. (A) Survival analysis of ARPC5 in CGGA database. (B) ROC curve analysis of ARPC5 at 1, 3, and 5 years in CGGA database. (C) Survival analysis of ARPC5 in TCGA database. (D) ROC curve analysis of ARPC5 at 1, 3, and 5 years in TCGA database. Survival analysis of the signature in patients stratified by grade (E, F), gender (G, H), age (I, J), IDH mutation status (K, L), 1p/19q codeletion status (M, N), and MGMTp methylation status (O, P) in CGGA database

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing, Mutagenesis, Methylation

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 3 Prognostic significance of ARPC5 and its association with drug sensitivity in glioma. (A) Univariate regression analysis of prognosis in CGGA data base. (B) Multivariate analysis of prognosis in CGGA database. (C) The nomogram to predict the association between ARPC5 expression and OS was de veloped using the CGGA dataset. (D) The calibration curve for the nomogram-predicted OS. ARPC5 affects the sensitivity to 5-fluorouracil (E), bleomycin (F), etoposide (G), crizotinib (H), sorafenib (I), and erlotinib (J)

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 4 Enrichment analysis identifies ARPC5-associated signaling pathways. (A) Heatmap for DEGs generated by compare the difference of ARPC5 ex pression in glioma from the CGGA dataset. (B) Volcano plots illustrated all DEGs. Barplot showing the GO (C) and KEGG (D) analysis for ARPC5 in glioma. (E) GO functional annotation of ARPC5 in glioma from GSEA. (F) KEGG pathway analysis of ARPC5 in glioma from GSEA.

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Protein-Protein interactions, Generated, Functional Assay

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 5 Investigations the expression of ARPC5 in glioma through single-cell analysis. (A) Through dimension reduction analysis of CGGA single-cell data, TSNE plot depicted that 6,148 cells were classified into 16 clusters. (B) The violin plot showed the expression of ARPC5 in each type of cluster. (C) Sixteen clusters were divided into 5 types of cells: astrocyte, macrophage, monocyte, epithelial and T cell. (D) The scatter plot shows the expression of ARPC5 in each cell

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing, Single-cell Analysis

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 6 Correlations of ARPC5 expression with immunity-related indexes. (A) Violin plot showed the correlation between immune classification and tumor purity. The immune infiltration models of low- and high-ARPC5 were detected through ssGSEA methods in glioma from the TCGA database. (C) Violin plot showed the correlation between ARPC5 with stromal scores, immune scores, and ESTIMATE scores. (D) Lollipop showed the correlation between ARPC5 with infiltrating immune cells. Scatter plot showed the correlation between ARPC5 with TMB (E) and MSI (F). (G-J) Violin plot showed the correla tion between ARPC5 with immunotherapy

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 7 Effect of ARPC5 on the expression of CD3 and prognosis. (A) Representative images revealed the relationships between the ARPC5 expression and T cell marker CD3 in gliomas. (B) Survival analysis of ARPC5 in glioma chip ZL-BraG180sur01.

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Expressing, Marker

Journal: BMC cancer

Article Title: ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas.

doi: 10.1186/s12885-023-11433-w

Figure Lengend Snippet: Fig. 8 Effect of ARPC5 on the proliferation and migration of glioma cells. (A) The expression level of ARPC5 protein was detected in LN229 and U251 cells after shRNA interference, GAPDH was used to confirm equal protein loading. (B) RT-qPCR verified the expression efficiency of ARPC5 in LN229 and U251 cells after shRNA interference. Growth curve assessing the effect of ARPC5 on the proliferation of LN229 (C) and U251 (D) cells. (E) The representa tive image showed the clone formation ability of LN229 and U251 cells after transfection. (F) Transwell analysis was further used to examine the effect of ARPC5 on migration of LN229 and U251 cells, and the number of migrating cells was quantitatively analyzed. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Glioma tissue samples and glioma chip ZLBraG180sur01 were incubated with ARPC5 antibody (16717-1-AP, proteintech, Wuhan, China) and CD3 antibody (17617-1-AP, proteintech, Wuhan, China).

Techniques: Migration, Expressing, shRNA, Quantitative RT-PCR, Transfection

Journal: Heliyon

Article Title: LncBIRC3-OT promotes the malignant progression of glioma by interacting with RELA to upregulate stanniocalcin-1 expression

doi: 10.1016/j.heliyon.2023.e21777

Figure Lengend Snippet: LncBIRC3-OT is required for malignant behaviours of glioma cells. (A) LncBIRC3-OT was silenced in U87 and U251 cells by shRNAs. (B) Cell viability was detected in lncBIRC3-OT-depleted cells by CCK-8 assay. (C) LncBIRC3-OT depletion causes a diminished colony-forming capacity in U87 and U251 cells. (D) Cell migration was detected in lncBIRC3-OT-depleted cells by wound-healing assays. (E) Cell invasion was analyzed in lncBIRC3-OT-silenced U87 and U251 cells by Transwell invasion assay. (F) N-cadherin, Vimentin, and E-cadherin were analyzed in lncBIRC3-OT-depleted cells by Western blot analysis. Uncropped images of blots are shown in Supplementary material. Data were shown as mean ± SD from three independent experiments. **P < 0.01, ***P < 0.001 vs. the NC groups.

Article Snippet: The primary antibodies used in this study are listed as follows: E-cadherin (1:5000, #60335-1-lg), N-cadherin (1:2000, #66219-1-lg), Vimentin (1:2000, #60330-1-lg), RELA (1:1000, #66535-1-lg), STC1 (1:1000, #20621-1-AP) and GAPDH (1:10000, #60004-1-lg) were obtained from Proteintech (Wuhan, China).

Techniques: CCK-8 Assay, Migration, Transwell Invasion Assay, Western Blot

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Knockout of α-calcitonin gene-related peptide attenuates cholestatic liver injury by differentially regulating cellular senescence of hepatic stellate cells and cholangiocytes

doi: 10.1038/s41374-018-0178-5

Figure Lengend Snippet: [A] Cellular senescence was enhanced in BDL WT mice compared to WT mice but reduced in BDL α-CGRP −/− mouse liver compared to BDL WT mice, which was verified by SA-β-gal staining in liver sections (n=4, Orig., magnification, 40×; scale bar=100μm). [B-C] The mRNA expression of [B] p16, p21 and CCL2 and PAI-1 as well as [C] p16 protein levels were increased in total liver from BDL WT mice compared to WT mice but decreased in total liver from BDL α-CGRP −/− mice compared to BDL WT mice (n=3). * p <0.05 vs. WT mice; # p <0.05 BDL WT mice.

Article Snippet: The mouse antibody against p16 (sc-166760) was purchased from Santa Cruz Biotechnology.

Techniques: Staining, Expressing

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Knockout of α-calcitonin gene-related peptide attenuates cholestatic liver injury by differentially regulating cellular senescence of hepatic stellate cells and cholangiocytes

doi: 10.1038/s41374-018-0178-5

Figure Lengend Snippet: [A] The mRNA expression of α-SMA, Col1α1 and Fn1 was decreased in cholangiocytes from BDL α-CGRP −/− mice compared to BDL WT mice (n=4). [B] The mRNA expression of p16, p21 and PAI-1 was decreased in cholangiocytes from BDL α-CGRP −/− mice compared to BDL WT mice (n=4). [C] The mRNA expression of SASP markers such as IL-6, IL-8, IL1-β and MMP2 was decreased in cholangiocytes from BDL α-CGRP −/− mice compared to BDL WT mice (n=4). [D] Immunofluorescence demonstrated that p16 protein expression was decreased in cholangiocytes from BDL α-CGRP −/− mice compared to BDL WT mice (n=3, Orig. magnification, 20×, scale bar= 50 μm). * p 0.05 vs. WT mice; # p <0.05 vs. BDL WT mice.

Article Snippet: The mouse antibody against p16 (sc-166760) was purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Immunofluorescence

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Knockout of α-calcitonin gene-related peptide attenuates cholestatic liver injury by differentially regulating cellular senescence of hepatic stellate cells and cholangiocytes

doi: 10.1038/s41374-018-0178-5

Figure Lengend Snippet: [A] The mRNA expression of Col1α1 was decreased in HSCs isolated from BDL α-CGRP −/− mice compared to BDL WT mice (n=3). [B] The p16 and p21 mRNA expression was increased in BDL α-CGRP −/− mice compared to BDL WT mice (n=3). [C] Immunofluorescent staining showed that p16 protein expression was decreased in HSCs from BDL WT mice compared to BDL α-CGRP −/− mice (n=3, Orig. magnification, 40×, scale bar= 25 μm). # p <0.05 vs. BDL WT mice.

Article Snippet: The mouse antibody against p16 (sc-166760) was purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Isolation, Staining

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Knockout of α-calcitonin gene-related peptide attenuates cholestatic liver injury by differentially regulating cellular senescence of hepatic stellate cells and cholangiocytes

doi: 10.1038/s41374-018-0178-5

Figure Lengend Snippet: [A] Representative immunofluorescence picture of receptor activity-modifying protein 1 (RAMP1) in HHSCs and IMCLs were shown (n=4, Orig., magnification. 40×; scale bar=50μm). [B-C] α-CGRP stimulated the expression of α-SMA and Col1 α 1 in both [B] IMCLs and [C] HHSCs, which was prevented by CGRP 8–37 (n=4). [D] The expression of p16 and p18 was decreased in HHSCs while increased in ICMLs simulated by α-CGRP; these effects were partly reversed by incubation with CGRP 8–37 (n=4). * p <0.05 vs. Basal; # p <0.05 vs. α-CGRP-treated group.

Article Snippet: The mouse antibody against p16 (sc-166760) was purchased from Santa Cruz Biotechnology.

Techniques: Immunofluorescence, Activity Assay, Expressing, Incubation

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Knockout of α-calcitonin gene-related peptide attenuates cholestatic liver injury by differentially regulating cellular senescence of hepatic stellate cells and cholangiocytes

doi: 10.1038/s41374-018-0178-5

Figure Lengend Snippet: [A-C] The mRNA expression of [A] TGF-β1, [B] TIMP1 and [C ] Fn1 increased in HHSCs stimulated with cholangiocyte supernatant from BDL WT mice compared to HHSCs stimulated with cholangiocyte supernatant from WT mice but decreased in HHSCs treated with cholangiocyte from BDL α-CGRP −/− mice compared to HHSCs stimulated with cholangiocyte supernatant from BDL WT mice (n=4). [D-F] The mRNA expression of [D] p16, [E] p18 and [F] p21 was increased in HHSCs treated with cholangiocyte supernatant from BDL α-CGRP −/− mice compared to HHSCs stimulated with BDL cholangiocyte supernatant (n=4). * p <0.05 vs. HHSCs treated with cholangiocyte supernatant from WT mice; # p <0.05 vs. HHSCs treated with cholangiocyte supernatant from BDL WT mice.

Article Snippet: The mouse antibody against p16 (sc-166760) was purchased from Santa Cruz Biotechnology.

Techniques: Expressing