Journal: BMC Genetics

Article Title: High-throughput analysis of candidate imprinted genes and allele-specific gene expression in the human term placenta

doi: 10.1186/1471-2156-11-25

Figure Lengend Snippet: Correlation of Sequenom and Illumina allele quantification . Pearson's correlation coefficient (r) is calculated for the allele-specific quantification on the two platforms and plotted against cDNA intensity (average log 2 fluorescence for the 23 placentas) on Illumina. The correlation drops when cDNA average intensity is lower than the 11.25 cut-off (dashed red line).

Article Snippet: Our criteria for ASE required that SNPs satisfy the following conditions: (1) average intensity across all samples greater than 11.25 (Illumina arbitrary fluorescence units); (2) at least 2 heterozygotes (based on BeadStudio calls from gDNA samples) with adjusted p -values less than 0.01 and absolute log-fold-changes greater than 0.585 and (3) at least 80% of homozygotes with adjusted p -values less than 0.01 and absolute log-fold-changes greater than 0.585.

Techniques: Fluorescence