aqp2 Search Results


aqp2  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology aqp2
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Aqp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/pmc12626674-21-1-3?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
aqp2 - by Bioz Stars, 2026-08
96/100 stars
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aquaporin 2 antibody  (Alomone Labs)
93
Alomone Labs aquaporin 2 antibody
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Aquaporin 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/pmc07566738-174-35-41?v=Alomone+Labs
Average 93 stars, based on 1 article reviews
aquaporin 2 antibody - by Bioz Stars, 2026-08
93/100 stars
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pcr  (OriGene)
94
OriGene pcr
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Pcr, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/pm42168243-264-12-15?v=OriGene
Average 94 stars, based on 1 article reviews
pcr - by Bioz Stars, 2026-08
94/100 stars
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human aqp2  (OriGene)
90
OriGene human aqp2
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Human Aqp2, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/pmc05042672-256-18-21?v=OriGene
Average 90 stars, based on 1 article reviews
human aqp2 - by Bioz Stars, 2026-08
90/100 stars
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anti pig aqp2  (Atlas Antibodies)
93
Atlas Antibodies anti pig aqp2
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Anti Pig Aqp2, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/pmc09232580__41467_2022_31388_MOESM18_ESM-39-0-5?v=Atlas+Antibodies
Average 93 stars, based on 1 article reviews
anti pig aqp2 - by Bioz Stars, 2026-08
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anti phospho aqp2 s256  (St Johns Laboratory)
91
St Johns Laboratory anti phospho aqp2 s256
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Anti Phospho Aqp2 S256, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/10__26420_slash_jdismarkers__2023__1057-112-38-40?v=St+Johns+Laboratory
Average 91 stars, based on 1 article reviews
anti phospho aqp2 s256 - by Bioz Stars, 2026-08
91/100 stars
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rabbit anti aquaporin 2  (OriGene)
90
OriGene rabbit anti aquaporin 2
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Rabbit Anti Aquaporin 2, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/pm35264234-219-39-43?v=OriGene
Average 90 stars, based on 1 article reviews
rabbit anti aquaporin 2 - by Bioz Stars, 2026-08
90/100 stars
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mouse plasmids for aqp2  (OriGene)
93
OriGene mouse plasmids for aqp2
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Mouse Plasmids For Aqp2, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/pmc12767696-64-42-47?v=OriGene
Average 93 stars, based on 1 article reviews
mouse plasmids for aqp2 - by Bioz Stars, 2026-08
93/100 stars
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rabbit polyclonal antibody  (OriGene)
91
OriGene rabbit polyclonal antibody
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Rabbit Polyclonal Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/pmc10680582-104-5-14?v=OriGene
Average 91 stars, based on 1 article reviews
rabbit polyclonal antibody - by Bioz Stars, 2026-08
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anti aqp2  (St Johns Laboratory)
91
St Johns Laboratory anti aqp2
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Anti Aqp2, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/10__26420_slash_jdismarkers__2023__1057-112-31-32?v=St+Johns+Laboratory
Average 91 stars, based on 1 article reviews
anti aqp2 - by Bioz Stars, 2026-08
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anti aquaporin 2 atto 550  (Alomone Labs)
90
Alomone Labs anti aquaporin 2 atto 550
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Anti Aquaporin 2 Atto 550, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/pmc04861333-92-10-12?v=Alomone+Labs
Average 90 stars, based on 1 article reviews
anti aquaporin 2 atto 550 - by Bioz Stars, 2026-08
90/100 stars
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anti rabbit polyclonal aquaporin 2 antibody  (Alomone Labs)
93
Alomone Labs anti rabbit polyclonal aquaporin 2 antibody
Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, <t>AQP2)</t> markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.
Anti Rabbit Polyclonal Aquaporin 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqp2/pm12969435-50-32-37?v=Alomone+Labs
Average 93 stars, based on 1 article reviews
anti rabbit polyclonal aquaporin 2 antibody - by Bioz Stars, 2026-08
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Image Search Results


Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, AQP2) markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.

Journal: Kidney360

Article Title: A Novel Human Distal Tubuloid-on-a-Chip Model for Investigating Sodium and Water Transport Mechanisms

doi: 10.34067/KID.0000000992

Figure Lengend Snippet: Expression profile of distal nephron markers in differentiated tubuloids. (A) Left: Schematic illustration of DNS highlighting the TAL of the loop of Henle (TAL; SLC12A1/NKCC2) and CD (SCNN1a/ENaC, AQP2) markers. Right: qPCR analysis showing segment-specific gene expression across four donors after differentiation (fold change relative to basal medium, log scale, Mean±SD). Samples from five chips per donor were pooled to create each replicate, with three independent samples per condition for each of the four donors. (B) Immunofluorescence analysis of tubuloid cultures (20× magnification). Upper panels show full-length tubules; lower panels show magnified regions (dotted boxes). Staining shows expression of ZO-1 (tight junctions), ECAD (distal tubule), NKCC2 (TAL), and AQP2 (CD), all counterstained with DNA (representative images, n =2–3). (C) Comparative gene expression analysis between 3D (OrganoPlate) and 2D (tubuloids grown on static 24-well plates) culture conditions across four donors (fold change, n =3). Comparisons made within each donor due to variability in baseline expression. All qPCR data were normalized to the housekeeping gene GAPDH and expressed as fold change relative to the corresponding 2D static culture condition for each donor. 2D, two-dimensional; CD, collecting duct; DCT, Distal convoluted tubule; DNS, distal nephron segments; ECAD, E-cadherin; ENaC, epithelial sodium channel; PT, proximal tubule; TAL, thick ascending limb.

Article Snippet: , AQP2 , Santa Cruz Biotechnology , Rabbit , — , Sc-515770 , 1:300.

Techniques: Expressing, Gene Expression, Immunofluorescence, Staining