Journal: Frontiers in Oncology

Article Title: CD47 Enhances Cell Viability and Migration Ability but Inhibits Apoptosis in Endometrial Carcinoma Cells via the PI3K/Akt/mTOR Signaling Pathway

doi: 10.3389/fonc.2020.01525

Figure Lengend Snippet: CD47 up-regulation inhibited apoptosis in HEC-1A and Ishikawa cells. (A,B) Up-regulation of CD47 inhibited cell apoptosis in Ishikawa cell line. (C,D) CD47 overexpression inhibited cell apoptosis in HEC-1A cell line. * p < 0.05.

Article Snippet: The cell cycle of transfected HEC-1A and Ishikawa cells were analyzed using the Cell Cycle and Apoptosis Analysis Kit (Beyotime Biotechnology, Shanghai, China), according to the manufacturer's protocol.

Techniques: Over Expression

Journal: Materials Today Bio

Article Title: Construction of dual-targeted liposomes loaded with celastrol and their application in treating intrahepatic cholangiocarcinoma

doi: 10.1016/j.mtbio.2025.101581

Figure Lengend Snippet: HCTL induces mitochondrial apoptosis in HuCCT-1 cells. (A) Analysis of active oxygen content (a, b, c, d, and e are the fluorescence photos of active oxygen in the control group, free Cela group, CL group, CTL group and HCTL respectively); (B) and (C) is the quantitative analysis chart of active oxygen flow cytometry; (D) Fluorescence image of mitochondrial membrane potential; (E) Flow cytometry quantitative pictures of mitochondrial membrane potential; (F) Intracellular ATP content; (G) and (H) Mitochondrial apoptosis-related proteins were evaluated by Western blot analysis for relative expression levels of proteins. Data are expressed as mean ± SD (n = 3), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 compared to control.

Article Snippet: The assay kit for cell cycle and apoptosis was acquired from GBCBIO Technologies.

Techniques: Fluorescence, Control, Flow Cytometry, Membrane, Western Blot, Expressing

Journal: Materials Today Bio

Article Title: Construction of dual-targeted liposomes loaded with celastrol and their application in treating intrahepatic cholangiocarcinoma

doi: 10.1016/j.mtbio.2025.101581

Figure Lengend Snippet: (A) Cell viability of HuCCT-1 cells after incubation with different formulations. Data are expressed as mean ± SD (n = 6); (B) Nuclear morphology of HuCCT-1 cells after 4 h of treatment with four preparations (a, b, c, d, and e are Hoechst staining of the control group, free Cela, CL, CTL, and HCTL respectively). (C) and (D) Apoptosis rate of HuCCT-1 cells after incubation with different preparations for 24 h. Data are expressed as mean ± SD (n = 3). ∗∗ p < 0.01, ∗∗∗ p < 0.001, ### p < 0.001 compared to control group.

Article Snippet: The assay kit for cell cycle and apoptosis was acquired from GBCBIO Technologies.

Techniques: Incubation, Staining, Control

Journal: Oncotarget

Article Title: Overexpression of RPS27a contributes to enhanced chemoresistance of CML cells to imatinib by the transactivated STAT3

doi: 10.18632/oncotarget.7888

Figure Lengend Snippet: ( A ) Relative STAT3 and RPS27a mRNA expression was assessed in K562 cells transfected with pcDNA3.1-STAT3 plasmids by qRT-PCR. ( B ) Lysates of K562 cells transfected with indicated plasmids were analyzed for STAT3, p-STAT3 and RPS27a protein by Western blot. ( C ) IC50s of K562-con and K562-STAT3 cells to imatinib at 48 h and 72 h was detected by MTT assays. ( D ) K562-con and K562-STAT3 cells were treated with or without 0.5 μM imatinib and 1.0 μM imatinib for 48 h and subjected to cell apoptosis analysis by flow cytometry analysis of Annexin-V labeling. Columns and bars represent mean from 3 parallel experiments and SD, respectively. * P < 0.05; ** P < 0.01.

Article Snippet: Phosphatidylserine externalization was analyzed with Annexin V-Alexa Fluor 647-A/PI Apoptosis Analysis Kit by a FACS Calibur flow cytometer (BD, USA) for cell apoptosis.

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot, Flow Cytometry, Labeling

Journal: Oncotarget

Article Title: Overexpression of RPS27a contributes to enhanced chemoresistance of CML cells to imatinib by the transactivated STAT3

doi: 10.18632/oncotarget.7888

Figure Lengend Snippet: ( A ) Lysates of K562-STAT3 cells incubated with or without WP1066 were analyzed for STAT3, p-STAT3 and RPS27a protein by Western blot. ( B ) K562-STAT3 cells were treated with or without 0.5 μM imatinib and 1.0 μM imatinib in the presence of 5 μM WP1066 for 48 h and subjected to cell apoptosis analysis by flow cytometry analysis of Annexin-V labeling. ( C ) Relative STAT3 and RPS27a mRNA expression was assessed in K562-STAT3 cells transfected with RPS27a shRNA plasmids by qRT-PCR. ( D ) Lysates of K562-STAT3-RPS27a-scr and K562-STAT3-RPS27a-sh cells were analyzed for STAT3, p-STAT3 and RPS27a protein by Western blot. ( E ) IC50s of K562-STAT3-RPS27a-scr and K562-STAT3-RPS27a-sh cells to imatinib at 48 h and 72 h was detected by MTT assays. ( F ) K562-STAT3-RPS27a-scr and K562-STAT3-RPS27a-sh cells were treated with or without 0.5 μM imatinib and 1.0 μM imatinib for 48 h and subjected to cell apoptosis analysis by flow cytometry analysis of Annexin-V labeling. Columns and bars represent mean from 3 parallel experiments and SD, respectively. * P < 0.05; ** P < 0.01.

Article Snippet: Phosphatidylserine externalization was analyzed with Annexin V-Alexa Fluor 647-A/PI Apoptosis Analysis Kit by a FACS Calibur flow cytometer (BD, USA) for cell apoptosis.

Techniques: Incubation, Western Blot, Flow Cytometry, Labeling, Expressing, Transfection, shRNA, Quantitative RT-PCR