Journal: Frontiers in Molecular Biosciences

Article Title: Serum proteomic profiling of precancerous gastric lesions and early gastric cancer reveals signatures associated with systemic inflammatory response and metaplastic differentiation

doi: 10.3389/fmolb.2024.1252058

Figure Lengend Snippet: DEPs coincident with the tissue expression. (A) Numbers of DEPs coincident with the tissue expression. (B) DEPs which were mainly expressed by enterocytes in single-cell RNA data of gastric tissue samples. (C) DEPs with high expression specificity in enterocyte in Human Protein Atlas data. (D) APOA4 levels in the gastric tissue samples. The barplot is the statistical result of the optical density analysis of IHC images. These tissue samples include NAG ( n = 10), IM ( n = 16), and GC ( n = 16). * p < 0.05; ** p < 0.01. (E) DEPs which were mainly expressed by epithelial cells in single-cell RNA data. (F) Fold changes of DEPs across cell types in pre-GC vs gastritis/normal comparison group. (G) DEPs coincident with the RNA datasets, GSE55696 and GSE130823. (H–J) ROC curves of the random forest models using the signature, APOA4, SERPINA4, and GCA.

Article Snippet: APOA4 antibody (17996-1-AP, Proteintech, USA) and COMP antibody (28369-1-AP, Proteintech, USA) were diluted at ratio 1:200.

Techniques: Expressing, Comparison

Journal: Proteomes

Article Title: The New and the Old: Platform Cross-Validation of Immunoaffinity MASS Spectrometry versus ELISA for PromarkerD, a Predictive Test for Diabetic Kidney Disease

doi: 10.3390/proteomes8040031

Figure Lengend Snippet: IAMS and ELISA working ranges and dilutions.

Article Snippet: A monoclonal antibody targeting the biomarker APOA4 was developed by the Monash Antibody Technologies Facility (MATF, Monash University, Melbourne, Australia) and the second antibody targeting APOA4 was developed by CDI laboratories (Mayaguez, PR, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: Proteomes

Article Title: The New and the Old: Platform Cross-Validation of Immunoaffinity MASS Spectrometry versus ELISA for PromarkerD, a Predictive Test for Diabetic Kidney Disease

doi: 10.3390/proteomes8040031

Figure Lengend Snippet: IAMS and ELISA intra-assay and inter-assay variability. Precision based on the average % CV.

Article Snippet: A monoclonal antibody targeting the biomarker APOA4 was developed by the Monash Antibody Technologies Facility (MATF, Monash University, Melbourne, Australia) and the second antibody targeting APOA4 was developed by CDI laboratories (Mayaguez, PR, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Intra Assay, Inter Assay

Journal: Proteomes

Article Title: The New and the Old: Platform Cross-Validation of Immunoaffinity MASS Spectrometry versus ELISA for PromarkerD, a Predictive Test for Diabetic Kidney Disease

doi: 10.3390/proteomes8040031

Figure Lengend Snippet: Difference plot ( A ) before Bland Altman correction comparing APOA4 concentrations derived from IAMS and ELISA platforms. Scatter plot ( B ) after adjusting ELISA concentration values with a Bland Altman analysis (adj = adjustment of +47.93 µg/mL to the ELISA value).

Article Snippet: A monoclonal antibody targeting the biomarker APOA4 was developed by the Monash Antibody Technologies Facility (MATF, Monash University, Melbourne, Australia) and the second antibody targeting APOA4 was developed by CDI laboratories (Mayaguez, PR, USA).

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Scientific Reports

Article Title: Aberrant Epicardial Adipose Tissue Extracellular Matrix Remodeling in Patients with Severe Ischemic Cardiomyopathy: Insight from Comparative Quantitative Proteomics

doi: 10.1038/srep43787

Figure Lengend Snippet: ( a ) Flowchart of the quantitative proteomics experiments; ( b ) A total of 2052 proteins was successfully quantified in the two experiment replicates, among which 1649 proteins were overlapped; ( c) Pearson Correlation analysis shows a Pearson Correlation Coefficient (PCC) of 0.76 for the 1649 overlapping proteins in the two replicates; ( d) Gaussian distribution of the quantitative data (Log 2 (Ratio)) shared by the two experimental replicates, the mean value was 0.0035 and SD value was 0.538; ( e) The protein levels of APOA4, CKM, CA2, TNNI3, and MYL3 in EAT of normal donors and ICM patients (n = 6–7 samples); ( f) The decreased proteins (FASN, CRP, and GLUL) confirmed by western blots (n = 6–7 samples). The β-actin was served as loading control.

Article Snippet: Antibodies against APOA4 (D221656), CKM (D260075), CA2 (D120344), TNNI3 (D120341), MYL3 (D122718), CRP (D120482), FASN (D262701), GLUL (D122427), A2M (D162821), and PLG (D262067) were obtained from BBI Life Sciences.

Techniques: Quantitative Proteomics, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112)

doi: 10.3390/ijms22094578

Figure Lengend Snippet: Genes highly induced by rh-HGF in the murine liver. Signal values at each timepoint show the average obtained from 3 mice which were sacrificed at designated timepoint before or after rh-HGF administration (1.5 mg/kg).

Article Snippet: Murine APOA4 was detected by anti-murine APOA4 antibody (GeneTex, Irvine, CA, USA) followed by peroxidase-conjugated anti-goat secondary antibodies (Invitrogen, Carlsbad, CA, USA).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112)

doi: 10.3390/ijms22094578

Figure Lengend Snippet: Induction of human APOA4 by rh-HGF, and its c-Met dependency. ( A ) Relative expression of APOA4 mRNA compared to the expression of 18S rRNA in human hepatocytes, ( B ) Relative concentration of APOA4 protein in supernatants ** p < 0.01, *** p < 0.001, **** p < 0.0001: comparison between vehicle control (incubation medium) or rh-HGF alone-treated group (Dunnett’s test). # p < 0.05: comparison between rh-HGF alone-treated group and PF-04217903-rh-HGF co-culture group (unpaired t test). The data are presented as mean ± SEM ( n = 3). The median value of the triplicate analyses was used for calculation. ( C ) Analysis of APOA4 protein levels in the supernatants of the culture using Western blot. The picture shows a representative batch of hepatocytes in triplicate.

Article Snippet: Murine APOA4 was detected by anti-murine APOA4 antibody (GeneTex, Irvine, CA, USA) followed by peroxidase-conjugated anti-goat secondary antibodies (Invitrogen, Carlsbad, CA, USA).

Techniques: Expressing, Concentration Assay, Comparison, Control, Incubation, Co-Culture Assay, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112)

doi: 10.3390/ijms22094578

Figure Lengend Snippet: Expression of Apoa4 mRNA in the liver and small intestine after rh-HGF administration in mice. ( A ) Liver, ( B ) Small intestine. Relative expression of Apoa4 mRNA against the expression of Hprt mRNA was calculated using the median value of the triplicate analyses * p < 0.05, *** p < 0.001, **** p < 0.0001: comparison between the vehicle control (PBS)- and rh-HGF-treated groups (Dunnett’s test). The data are presented as mean ± SEM ( n = 3).

Article Snippet: Murine APOA4 was detected by anti-murine APOA4 antibody (GeneTex, Irvine, CA, USA) followed by peroxidase-conjugated anti-goat secondary antibodies (Invitrogen, Carlsbad, CA, USA).

Techniques: Expressing, Comparison, Control

Journal: International Journal of Molecular Sciences

Article Title: Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112)

doi: 10.3390/ijms22094578

Figure Lengend Snippet: c-Met dependent induction of murine APOA4. Twenty-four hours after rh-HGF administration with or without PF-04217903 treatment, the mice were sacrificed, and their livers and bloods were collected for the analyses. ( A ) Relative expression of Apoa4 mRNA in the liver compared with the expression of Hprt mRNA in mice, ( B ) Relative value of APOA4 protein in serum * p < 0.05, ** p < 0.01: comparison between vehicle control (PBS) and rh-HGF alone-treated groups (Dunnett’s test), # p < 0.05, ## p < 0.01, ### p < 0.001: comparison between rh-HGF alone-treated group and PF-04217903- rh-HGF co-administered group (unpaired t test). The data are presented as mean ± SEM (n = 3). The median value of the triplicate analyses was used for calculation. ( C ) Analysis of APOA4 protein levels in serum using Western blot. The picture shows a representative image of lanes showing individual animals.

Article Snippet: Murine APOA4 was detected by anti-murine APOA4 antibody (GeneTex, Irvine, CA, USA) followed by peroxidase-conjugated anti-goat secondary antibodies (Invitrogen, Carlsbad, CA, USA).

Techniques: Expressing, Comparison, Control, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112)

doi: 10.3390/ijms22094578

Figure Lengend Snippet: rh-HGF-mediated induction of APOA4 protein in anti-Fas-induced murine ALF model. rh-HGF was injected intravenously one hour prior to the anti-Fas injection. Five hours after anti-Fas administration, the mice were sacrificed and their blood collected for the analyses. ( A ) Relative value of APOA4 protein in serum. PBS was administered in the vehicle control group (rh-HGF, 0 mg/kg). ** p < 0.01, **** p < 0.0001: comparison between the vehicle control- and rh-HGF-treated groups (Dunnett’s test). The data are presented as mean ± SEM ( n = 5–8). ( B ) A representative image of serum APOA4 levels by Western blot. Each lane shows individual animals.

Article Snippet: Murine APOA4 was detected by anti-murine APOA4 antibody (GeneTex, Irvine, CA, USA) followed by peroxidase-conjugated anti-goat secondary antibodies (Invitrogen, Carlsbad, CA, USA).

Techniques: Injection, Control, Comparison, Western Blot