Journal: Archives of Medical Science : AMS

Article Title: Expression of candidate genes associated with obesity in peripheral white blood cells of Mexican children

doi: 10.5114/aoms.2016.58126

Figure Lengend Snippet: Studied candidate genes associated with obesity

Article Snippet: APOA4 , Apolipoprotein A-IV , NM_000482.3 , Hs00166636_m1 , Obesity , .

Techniques:

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: MicroRNA-3194-3p inhibits metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by decreasing Wnt/β-catenin signaling through targeting BCL9.

doi: 10.1080/21691401.2019.1670190

Figure Lengend Snippet: Figure 3. miR-3194-3p inhibits the EMT phenotype of HCC cells. (A) Western blot analysis of epithelial marker E-cadherin, and mesenchymal marker N-cadherin and Vimentin after overexpressing miR-3194-3p in MHCC-97H cells. (B) Western blot analysis of E-cadherin, N-cadherin and Vimentin after down-regulating miR-3194-3p in Hep3B cells. GAPDH was used as an internal control. (C) IF staining of E-cadherin and Vimentin after over-expression of miR-3194-3p in MHCC-97H cells. (D) IF staining of E-cadherin and Vimentin after inhibition of miR-3194-3p in Hep3B cells. (E) Immunohistochemistry of E-cadherin and Vimentin were shown and com- pared between tissues of high miR-3194-3p level and those of low miR-3194-3p level. p < .05.

Article Snippet: E-cadherin (Mouse Monoclonal, 13–5700), N-cadherin (Mouse Monoclonal, MA1- 91128), Vimentin (Mouse Monoclonal, MA5-11883), beta-catenin (Rabbit Monoclonal, 8480S) and BCL9 (Rabbit Polyclonal, PA5-51747) antibodies were purchased from CST Signaling Company.

Techniques: Western Blot, Marker, Control, Staining, Over Expression, Inhibition, Immunohistochemistry

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: MicroRNA-3194-3p inhibits metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by decreasing Wnt/β-catenin signaling through targeting BCL9.

doi: 10.1080/21691401.2019.1670190

Figure Lengend Snippet: Figure 7. miR-3194-3p inhibits the lung metastasis of HCC cells in nude mice. (A) Representative HE staining of lung metastasis of MHCC-97H-Control cells and MHCC-97H-miR-3194-3p cells. (B) Immunohistochemistry of BCL9, EMT markers suggest that MHCC-97H-miR-3194-3p shows a weaker staining of BCL9, mesenchy- mal marker Vimentin, but a stronger epithelial E-cadherin, than MHCC-97H-miR-control cells. (C) Representative HE staining of lung metastasis of Hep3B-NC cells and Hep3B-anti-miR-3194-3p cells. (D) Immunohistochemistry of BCL9, EMT markers suggest that Hep3B-anti-miR-3194-3p shows a stronger staining of BCL9, mes- enchymal marker Vimentin, but a weaker epithelial E-cadherin, than Hep3B-anti-miR-NC cells. n ¼ 3 independent experiments.

Article Snippet: E-cadherin (Mouse Monoclonal, 13–5700), N-cadherin (Mouse Monoclonal, MA1- 91128), Vimentin (Mouse Monoclonal, MA5-11883), beta-catenin (Rabbit Monoclonal, 8480S) and BCL9 (Rabbit Polyclonal, PA5-51747) antibodies were purchased from CST Signaling Company.

Techniques: Staining, Control, Immunohistochemistry, Marker

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: MicroRNA-3194-3p inhibits metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by decreasing Wnt/β-catenin signaling through targeting BCL9.

doi: 10.1080/21691401.2019.1670190

Figure Lengend Snippet: Figure 9. miR-3194-3p is down-regulated by hypoxia and mediates the promoting effects of hypoxia on metastasis and EMT of HCC cell. (A) b-catenin protein levels in HCC cells transfected with miR-3194-3p inhibitor or miR-3194-3p over-expression vectors. (B) The expression level of HIF-1a in different time points in hypoxia condition. (C) The expression level of miR-3194-3p in Hep3B cells cultured in normoxia and hypoxia. (D) Transwell assays for Hep3B cells in normoxia and Hep3B cells and Hep3B cells transfected with miR-3194-3p in hypoxia. p < .01. (E) The expression of BCL9, E-cadherin, N-cadherin and Vimentin of Hep3B cells in nor- moxia and Hep3B cells and Hep3B cells transfected with miR-3194-3p in hypoxia. p < .05. n¼six independent experiments.

Article Snippet: E-cadherin (Mouse Monoclonal, 13–5700), N-cadherin (Mouse Monoclonal, MA1- 91128), Vimentin (Mouse Monoclonal, MA5-11883), beta-catenin (Rabbit Monoclonal, 8480S) and BCL9 (Rabbit Polyclonal, PA5-51747) antibodies were purchased from CST Signaling Company.

Techniques: Transfection, Over Expression, Expressing, Cell Culture

Journal: Scientific Reports

Article Title: The Adult Murine Intestine is Dependent on Constitutive Laminin-γ1 Synthesis

doi: 10.1038/s41598-019-55844-x

Figure Lengend Snippet: RT-PCR Analysis of Epithelial and Mesenchymal Transcripts.

Article Snippet: TaqMan primer sets were all from Thermofisher Scientific, Waltham MA: Lama1 (Mm01226102_m1), Lama2 (Mm00550083_m1), Lama3 (Mm01254735_m1), Lama4 (Mm01193660_m1), Lama5 (Mm01222029_m1), Lamb1 (Mm00801853_m1), Lamb2 (Mm00493080_m1), Lamb3 (Mm00493108_m1), Lamc1 (Mm00711820_m1), Lamc2 (Mm00500494_m1), Lamc3 (Mm01324510_m1), Lactase (Mm01276696_m1), Muc2 (Mm01276696_,1), Lysozyme (Mm00657323_m1), ChgA (Mm00514341_m1), Lgr5 (Mm00438890_m1), Olfm4 (Mm01320260_m1), mTert (Mm01352136_s1), HopX (Mm00558630_m1), NGN3 (Mm00437606_s1), Gata4 (Mm00437606_s1), Gata6 (Mm00802632_m1), Ihh (Mm00439613_m1), Ptchd1 (Mm00436026_m1), Abcg5 (Mm00433937_m1), Abcg8 (Mm00433937_m1), Ada (Mm00545720_m1), Akr1b8 (Mm00484314_m1), Apoa4 (Mm00431814_m1), ApoB (Mm00431814_m1), ApoC2 (Mm00433937_m1), ApoC3 (Mm00433937_m1), Asah2 (Mm00479659_m1), Cck (Mm00446170_m1), CD36 (Mm01135198_m1), Cftr (Mm00445197_m1), Dnase1 (Mm00445197_m1), Enpp7 (Mm01252812_m1), Fabp2 (Mm00433188_m1), Hsd17b6 (Mm00457343_m1), Pdx1 (Mm00435565_m1), Slc2a1 (Mm00441473_m1), Slc2a2 (Mm00441473_m1), Slc2a5 (Mm00433937_m1), Slc2a8 (Mm00433937_m1), Slc5a1 (Mm00433937_m1), Slc5a11 (Mm00433937_m1), Sst (Mm01436671_m1), and Ugt2a3 (Mm01436671_m1).

Techniques: Mutagenesis

Journal: Frontiers in Molecular Biosciences

Article Title: Serum proteomic profiling of precancerous gastric lesions and early gastric cancer reveals signatures associated with systemic inflammatory response and metaplastic differentiation

doi: 10.3389/fmolb.2024.1252058

Figure Lengend Snippet: DEPs coincident with the tissue expression. (A) Numbers of DEPs coincident with the tissue expression. (B) DEPs which were mainly expressed by enterocytes in single-cell RNA data of gastric tissue samples. (C) DEPs with high expression specificity in enterocyte in Human Protein Atlas data. (D) APOA4 levels in the gastric tissue samples. The barplot is the statistical result of the optical density analysis of IHC images. These tissue samples include NAG ( n = 10), IM ( n = 16), and GC ( n = 16). * p < 0.05; ** p < 0.01. (E) DEPs which were mainly expressed by epithelial cells in single-cell RNA data. (F) Fold changes of DEPs across cell types in pre-GC vs gastritis/normal comparison group. (G) DEPs coincident with the RNA datasets, GSE55696 and GSE130823. (H–J) ROC curves of the random forest models using the signature, APOA4, SERPINA4, and GCA.

Article Snippet: APOA4 antibody (17996-1-AP, Proteintech, USA) and COMP antibody (28369-1-AP, Proteintech, USA) were diluted at ratio 1:200.

Techniques: Expressing, Comparison

Journal: Clinical and translational gastroenterology

Article Title: Serological Investigation of Persistent Villous Atrophy in Celiac Disease.

doi: 10.14309/ctg.0000000000000639

Figure Lengend Snippet: Figure 5. Determination of a panel of lipids, including total cholesterol (a), triglycerides (b), low-density lipoprotein cholesterol (LDL-C, c), high-density lipoprotein cholesterol (HDL-C, d), and apo A4 (e) in the nonceliac control, non-VA CeD, and VA CeD groups. (f) Heatmap and cluster analysis for apolipoproteins. MFI, median fluorescence intensity; VA, villous atrophy.

Article Snippet: Recombinant human apolipoprotein A-IV/apo A4 protein (R&D Systems, 9606-AP-050) was used to generate a standard curve.

Techniques: Control, Fluorescence