Journal: International Journal for Parasitology: Drugs and Drug Resistance
Article Title: TPT sulfonate, a single, oral dose schistosomicidal prodrug: In vivo efficacy, disposition and metabolic profiling
Figure Lengend Snippet: Relative ion abundance of TPT sulfonate metabolites from mouse plasma and a mouse hepatocyte digest. MS/MS peak areas are compared for metabolites observed in ( A ) mouse plasma 4 h after 100 mg/kg PO dosing and in ( B ) a mouse hepatocyte incubation after 20 min, using 400 μM TPT sulfonate and 3 × 10 6 cells/m. Solid colors represent metabolites depicted in Fig. 9 ; cross-hatching indicates metabolites differing from those in the figure by two mass units (possibly representing a gain or loss of a double bond) and vertical stripes indicate metabolites of unknown structure. Data were obtained via m/z 56 neutral loss scanning from m/z 100 to 320. The m/z 208 compound is not a metabolite per se , but a decomposition product that forms in the inlet to the API 4000 MS from the m/z 417 thiol dimer.
Article Snippet: 2.7 Positive ion-mode electrospray tandem MS of TPT sulfonate and its metabolites in mouse plasma and mouse hepatocyte digests The principal fragments of m/z 290 TPT sulfonate observed using an API 4000 LC-MS/MS system (SCIEX, Framingham, MA) (see description under Results) are m/z 234, 135, 120 and 91.
Techniques: Mass Spectrometry, Incubation